RESEARCH PAPER

Effects of Dietary Daylily Stem and Leaf Silage on Rumen Microflora Diversity of Tan Sheep

  • WANG Jin ,
  • ZHANG Lianquan ,
  • WANG Wenliang ,
  • ZHANG Jiupan ,
  • ZHAO Zhengwei ,
  • YANG Yuwei ,
  • MA Jifeng ,
  • ZHANG Junli ,
  • WANG Xiuqin ,
  • MA Qing
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  • 1. Institute of Animal Sciences, Ningxia Academy of Agriculture and Forestry Sciences, Yinchuan 750002, China;
    2. Ningxia Shuomu Yanchi Tan Sheep Breeding Co., Ltd., Wuzhong 751100, China

Received date: 2021-12-06

  Online published: 2022-07-14

Abstract

The purpose of this experiment was to investigate the effect of dietary adding different proportions of daylily stem and leaf silage on rumen microflora diversity of Tan sheep. Eighty healthy male Tan sheep lambs with similar body weight[(21.60±1.09) kg] were selected and randomly divided into 4 groups according to the body weight, each group contained 20 lambs. According to the proportion of daylily stem and leaf silage and corn silage in the diet, the control group was fed an experimental diet which contained 100% corn silage, the trial group 1 was fed an experimental diet which contained 20% daylily stem and leaf silage+80% corn silage, the trial group 2 was fed an experimental diet which contained 40% daylily stem and leaf silage+60% corn silage, and the trial group 3 was fed an experimental diet which contained 60% daylily stem and leaf silage+40% corn silage. The pre-experimental period lasted for 10 days, and the experimental period lasted for 90 days. The results showed as follows:1) there were no significant differences in concentration of nitrogen, acetic acid, propionic acid, butyric acid and valeric acid in rumen fluid among all groups (P>0.05), but the trial group 1 was the highest, which were increased by 28.65%, 36.34%, 26.70%, 41.62% and 3.00% compared with the control group. 2) The operational taxonomic units (OTU) number of trial groups was higher than that of the control group, and the order of OTU number was trial group 1 > trial group 3 > trial group 2 > control group. 3) The Ace, Chao1, Shannon and Simpson indexes of trail group 1 were the highest, and the Shannon index was significantly higher than that of the control group (P<0.05). 4) At the phylum level, the Firmicutes, Bacteroidetes, Patescibacteria, Actinobacteria, and Proteobacteria were the dominant flora in rumen of Tan sheep. Compared with the control group, the Bacteroidetes relative abundance of trial groups was increased (P>0.05), and the trial group 1 was the highest; and the Firmicutes, Patescibacteria, Actinomycetes, Proteobacteria relative abundances were decreased (P>0.05). 5) At the genus level, the Ruminococcus, Prevotella and Candidatus_Saccharimonas were the dominant flora in rumen of Tan sheep. Compared with the control group, the Ruminococcus, Olsenella and Lachnospiraceae_NK3A20_group relative abundances of trial groups were significantly decreased (P<0.05), and the Muribaculaceae relative abundance of trial group 1 was significantly increased (P<0.05). To sum up, when dietary adding 20% daylily stem and leaf silage+80% corn silage, it has little effect on the structure and function of rumen microbial flora of Tan sheep, at the same time, the species and diversity of rumen microbial flora of Tan sheep can be improved, and the structure and composition of rumen colony can be changed. In production practice, daylily stem and leaf silage can be used as a silage supplement, but the addition proportion should not be higher than 20%.

Cite this article

WANG Jin , ZHANG Lianquan , WANG Wenliang , ZHANG Jiupan , ZHAO Zhengwei , YANG Yuwei , MA Jifeng , ZHANG Junli , WANG Xiuqin , MA Qing . Effects of Dietary Daylily Stem and Leaf Silage on Rumen Microflora Diversity of Tan Sheep[J]. Chinese Journal of Animal Nutrition, 2022 , 34(7) : 4550 -4561 . DOI: 10.3969/j.issn.1006-267x.2022.07.044

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