RESEARCH PAPER

Effects of Dietary Cholesterol Level on Growth Performance, Digestive Enzyme Activities, Antioxidant Capability and Lipid Metabolism Related Gene Expression of Procambarus clarkii

  • TIAN Hongyan ,
  • YANG Chengcong ,
  • ZHANG Chunnuan ,
  • WANG Aimin ,
  • LIU Fei ,
  • YANG Wenping ,
  • YU Yebing ,
  • REN Shengjie ,
  • ZHANG Wuxiao
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  • 1. College of Marine and Bioengineering, Yancheng Institute of Technology, Yancheng 224000, China;
    2. College of Animal Science and Technology, Henan University of Science and Technology, Luoyang 471023, China

Received date: 2021-12-26

  Online published: 2022-07-14

Abstract

In order to study the effects of dietary cholesterol level on the growth performance, body composition, digestive enzyme activities, antioxidant capacity and lipid metabolism related gene expression of Procambarus clarkii (P. clarkii), five diets with different levels of cholesterol (0, 0.25%, 0.50%, 0.75% and 1.00%) were designed and used in the present study. The measured cholesterol levels in the diets were 0.06%, 0.29%, 0.54%, 0.81% and 1.05%, respectively. A total of 225 P. clarkii with similar sizes[initial weight=(4.4±0.1) g] were randomly divided into 5 groups with 3 replicates in each group, and each replicate contained 15 shrimp tanks. After 6 weeks' feeding trial, samples were collected and tested. The results showed as follows:1) with the increasing of the dietary cholesterol level from 0 to 0.50%, the weight gain rate and lipase activity in the hepatopancreas, stomach and intestines of P. clarkii increased significantly (P<0.05), but decreased significantly as the dietary cholesterol level increasing from 0.50% to 1.00% (P<0.05). Dietary cholesterol level had no significant effects on the whole-body moisture, crude protein, ash and calcium contents among all groups (P>0.05). However, the crude fat content was significantly lower in the P. clarkii fed dietary containing less than 0.50% cholesterol (P<0.05). 2) In contrast to the malondialdehyde (MDA) content, superoxide dismutase (SOD) activity in blood lymphocyte of P. clarkii increased significantly with the dietary cholesterol level increasing from 0 to 0.50% (P<0.05), but then decreased significantly with the increasing dietary cholesterol level (P<0.05). 3) Compared with group C0, the mRNA relative expression level of carnitine palmitoyltransferase-Ⅰ (CPT-Ⅰ) in hepatopancreas decreased significantly with the dietary cholesterol level increasing (P<0.05). The mRNA relative expression levels of fatty acid-binding protein (FABP) and fatty acid transport protein (FATP) in hepatopancreas increased significantly (P<0.05) in the P. clarkii fed diet with cholesterol. In conclusion, P. clarkii fed diet with 0.50% cholesterol level can obtain the enhanced growth performance and lipase activity. Dietary cholesterol might affect the lipid metabolism through suppressing the β-oxidation and promoting the transport of fatty acids. In addition, low or excessive cholesterol both can reduce the antioxidant capacity in the blood lymphocyte of P. clarkii. Taken above, the dietary cholesterol suitable supplemental level for P. clarkii is 0.50% in this experiment, while the cholesterol measured level in the diet is 0.54%.

Cite this article

TIAN Hongyan , YANG Chengcong , ZHANG Chunnuan , WANG Aimin , LIU Fei , YANG Wenping , YU Yebing , REN Shengjie , ZHANG Wuxiao . Effects of Dietary Cholesterol Level on Growth Performance, Digestive Enzyme Activities, Antioxidant Capability and Lipid Metabolism Related Gene Expression of Procambarus clarkii[J]. Chinese Journal of Animal Nutrition, 2022 , 34(7) : 4630 -4641 . DOI: 10.3969/j.issn.1006-267x.2022.07.051

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