Gastric Evacuation Characteristic and Digestive Enzyme Activity Change of Largemouth Bass (Micropterus Salmoides) Fed Diets Supplemented with Small Peptides

CONG Xiangming; LI Xiang; HUA Xueming; MI Shuichao; HANG Ying; YI Wanting

doi:10.3969/j.issn.1006-267x.2022.07.052

2022 , Vol. 34 >Issue 7: 4642 - 4656

DOI: https://doi.org/10.3969/j.issn.1006-267x.2022.07.052

RESEARCH PAPER

Gastric Evacuation Characteristic and Digestive Enzyme Activity Change of Largemouth Bass (Micropterus Salmoides) Fed Diets Supplemented with Small Peptides

CONG Xiangming ,
LI Xiang ,
HUA Xueming ,
MI Shuichao ,
HANG Ying ,
YI Wanting

Expand

1. Centre for Research on Environmental Ecology and Fish Nutrition of the Ministry of Agriculture, Shanghai Ocean University, Shanghai 201306, China;
2. Key Laboratory of Freshwater Aquatic Genetic Resources, Ministry of Agriculture and Rural Affairs, Shanghai Ocean University, Shanghai 201306, China;
3. National Demonstration Center for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai 201306, China;
4. Changzhou Yayuan Biochemical Co., Ltd., Changzhou 213111, China

Received date: 2021-11-04

Online published: 2022-07-14

Fold

Abstract

In order to investigate the effects of the small peptides on gastric evacuation characteristic and digestive enzyme activity change in the gastrointestinal tract of largemouth bass (Micropterus salmoides), three experimental diets were prepared, D0⁺ diet was meet the nutritional requirements of largemouth bass (positive control group), D0^- diet reduced 3% dietary protein level based on the D0⁺ diet (negative control group) and the D2 diet was added 2% small peptides based on D0^- diet. The three diets were fed to healthy largemouth bass with the initial body weight of (21.27±0.17) g for 60 days with four replicates in each group and 45 individuals in each replicate. At the end of the feeding experiment, 80 fish with similar body weight of (85.58±1.81) g were selected from each group, and then were randomly distributed into 8 cages per group, 10 individuals per cage. The fish in all cages continued to be fed respectively with above three diets to apparent satiation once a day. At 0, 3, 6, 9, 12, 18, 24 and 30 h after feeding, the fish were sampled from one of the 8 cages in each group. The percentage of gastric content remaining, fitting degree of the three mathematical models to the gastric emptying curve of each group and changes of digestive enzyme activities of largemouth bass were compared after feeding the above three diets in different time periods. The results showed that the dry weight of gastric content were better fitting to the mathematical models, the gastric evacuation characteristic and the optimal model of the largemouth bass were not significantly affected by varying protein levels and supplementation of small peptides in the diet, and the gastric emptying changes were curve digestive pattern (slow-fast-slow) during the gastric evacuation process with not entirely same trend. The linear, exponential and square root models could fit the gastric emptying curve of largemouth bass well. The exponential model was the best model to simulate the gastric emptying pattern of the D0⁺ group, and the square root model was the best model to simulate the gastric emptying pattern of the D0^- and D2 groups. The theoretical time of 50% and 80% gastric evacuation was 8.14 and 19.04 h in D0⁺ group, 11.28 and 21.4 h in D0^- group, 10.21 and 19.67 h in D2 group, according to the best model calculation in each group. The activities of digestive enzymes (amylase, protease and lipase) in the gastrointestinal tract in the three groups were firstly increased and then decreased during gastric evacuation, and the activities of amylase, lipase and protease in the gastrointestinal tract in D0⁺ group reached the highest value at 9 h after ingestion, which were significantly higher than the other time in the same group (P<0.05). The activity of pepsin in D0^- and D2 groups reached the highest value at 9 h after ingestion. While the activities of amylase, lipase and protease in the gastrointestinal tract reached the highest value at 12 h after ingestion, which were significantly higher than the other time in the same group (P<0.05). In conclusion, changes in dietary protein levels and addition of small peptides can cause changes in gastrointestinal digestive enzyme to maintain a relatively constant gastric empting time, so there is no need to adjust feeding frequency. According to the production practice, it is recommended that the feeding interval of largemouth bass is 8 to 21 h, twice a day.

Key words： Micropterus salmoides; small peptide; gastric evacuation rate; digestive enzyme; feeding frequency

Cite this article

CONG Xiangming , LI Xiang , HUA Xueming , MI Shuichao , HANG Ying , YI Wanting . Gastric Evacuation Characteristic and Digestive Enzyme Activity Change of Largemouth Bass (Micropterus Salmoides) Fed Diets Supplemented with Small Peptides[J]. Chinese Journal of Animal Nutrition, 2022 , 34(7) : 4642 -4656 . DOI: 10.3969/j.issn.1006-267x.2022.07.052

References

[1] 张波,孙耀,唐启升.鱼类的胃排空率及其影响因素[J].生态学报,2001,21(4):665-670. ZHANG B,SUN Y,TANG Q S.Gastric evacuation rate of fish and its influence factors[J].Acta Ecologica Sinica,2001,21(4):665-670.(in Chinese)
[2] DUNBRACK R,GIGUōRE L A,ST-PIERRE J F.A comparison of gut evacuation models for larval mackerel (Scomber scombrus) using serial photography[J].Journal of Fish Biology,2009,74(4):906-920.

[3] MYCHEK-LONDER J G,BUNNELL D B.Gastric evacuation rate, index of fullness, and daily ration of Lake Michigan slimy (Cottus cognatus) and deepwater sculpin (Myoxocephalus thompsonii)[J].Journal of Great Lakes Research,2013,39(2):327-335.

[4] JOBLING M.Mathematical models of gastric emptying and the estimation of daily rates of food consumption for fish[J].Journal of Fish Biology,1981,19(3):245-257.

[5] 孙耀,刘勇,张波,等.渤、黄海4种小型鱼类摄食排空率的研究[J].海洋与湖沼,2002,33(6):679-684. SUN Y,LIU Y,ZHANG B,et al.Gastric evacuation rates of 4 small-size fish species in Bohai and Yellow seas[J].Oceanologia et Limnologia Sinica,2002,33(6):679-684.(in Chinese)
[6] SEYHAN K,GROVE D J.Food consumption of whiting,Merlangius merlangus,in the Eastern Irish Sea[J].Fisheries Research,1998,38(3):233-245.

[7] KHAN U,SEYHAN K.Gastric evacuation rates in farmed brook trout subjected to a range of feeding conditions fed commercial pellets[J].Aquaculture,2019,513:734390.
[8] LEE S M,HWANG U G,CHO S H.Effects of feeding frequency and dietary moisture content on growth,body composition and gastric evacuation of juvenile Korean rockfish (Sebastes schlegeli)[J].Aquaculture,2000,187(3/4):399-409.
[9] 管敏,张德志,饶军,等.饱食投喂频率对子二代中华鲟稚鱼生长及胃肠排空的影响[J].淡水渔业,2019,49(4):96-103. GUAN M,ZHANG D Z,RAO J,et al.Effects of satiation feeding frequency on growth and gastrointestinal evacuation characteristic of juvenile F2 Chinese sturgeon (Acipenser sinensis)[J].Freshwater Fisheries,2019,49(4):96-103.(in Chinese)
[10] 王伟,张凯强,温海深,等.投喂频率对花鲈幼鱼胃排空、生长性能和体组分的影响[J].中国海洋大学学报(自然科学版),2018,48(6):55-62. WANG W,ZHANG K Q,WEN H S,et al.Effects of feeding frequency on gastric evacuation, growth performance and body composition of juvenile Lateolabrax maculatus[J].Periodical of Ocean University of China,2018,48(6):55-62.(in Chinese)
[11] 李可贵,曹振东,付世建.鲇鱼幼鱼的胃排空率及其模型分析[J].重庆师范大学学报(自然科学版),2009,26(3):8-11. LI K G,CAO Z D,FU S J.Analysis of gastric evacuation rate and its mathematical model in Chinese catfish (Silurus asotus Linnaeus)[J].Journal of Chongqing Normal University(Natural Science Edition),2009,26(3):8-11.(in Chinese)
[12] 朱伟星,华雪铭,钟国防,等.斑点叉尾对全植物蛋白饲料的胃排空及消化道内容物特性研究[J].水产学报,2015,39(4):529-538. ZHU W X,HUA X M,ZHONG G F,et al.Patterns of gastric evacuation and digesta characteristics in the gastrointestinal tract of channel catfish (Ictalurus punctatus) when fed plant protein based diet[J].Journal of Fisheries of China,2015,39(4):529-538.(in Chinese)
[13] 黄铭,周演根,陈薛伟杰,等.两种规格虹鳟胃排空模型和投喂频率的研究[J].中国海洋大学学报(自然科学版),2020,50(12):33-39. HUANG M,ZHOU Y G,CHEN X W J,et al.Studies on gastric evacuation rate and feeding frequency of rainbow trout (Oncorhynchus mykiss) in two sizes[J].Periodical of Ocean University of China,2020,50(12):33-39.(in Chinese)
[14] 李志斐,龚望宝,王金林,等.冰鲜杂鱼和人工配合饲料对大口黑鲈肌肉品质及健康状况影响的评价[J].动物营养学报,2017,29(11):4180-4188. LI Z F,GONG W B,WANG J L,et al.Evaluation of effects of frozen fresh fish and artificial compound feed on muscle quality and health status of Micropterus salmoides[J].Chinese Journal of Animal Nutrition,2017,29(11):4180-4188.(in Chinese)
[15] 夏焱春,曹铮,蔺凌云,等.大口黑鲈主要病害研究进展[J].中国动物检疫,2018,35(9):72-76. XIA Y C,CAO Z,LIN L Y,et al.Research progress on main diseases of largemouth bass (Micropterus salmoides)[J].China Animal Health Inspection,2018,35(9):72-76.(in Chinese)
[16] 贺光祖.谷氨酰胺二肽对猪肠上皮细胞更新和蛋白质代谢的调控研究[D].硕士学位论文.长沙:湖南农业大学,2015. HE G Z.Effects of glutamine dipeptide on regeneration and protein metabolism of pig intestinal epithelial cells[D].Master's Thesis.Changsha:Hunan Agricultural University,2015.(in Chinese)
[17] LI X,WEI X,GUO X,et al.Enhanced growth performance,muscle quality and liver health of largemouth bass (Micropterus salmoides) were related to dietary small peptides supplementation[J].Aquaculture Nutrition,2020,26(6):2169-2177.

[18] 孙耀,马志敏,刘勇,等.东海、黄海不同生长时期鳀鱼的胃排空率[J].海洋学报2006,28(3):103-108. SUN Y,MA Z M,LIU Y,et al.The gastric evacuation rate of anchovy Engraulis japonicus in different growth stages in Huanghai Sea and East China Sea[J].Acta Oceanologica Sinica,2006,28(3):103-108.(in Chinese)
[19] 覃志彪,赵海祥,赵华林,等.投饲频率对瓦氏黄颡鱼(Pelteobagrus vachelli)幼鱼胃排空、生长效益及体组成影响[J].海洋与湖沼,2011,42(4):506-511. QIN Z B,ZHAO H X,ZHAO H L,et al.Effects of feeding frequency on gastric evacuation,growth performance and body composition of juvenile darkbarbel catfish (Pelteobagrus vachelli Richardson)[J].Oceanologia et Limnologia Sinica,2011,42(04):506-511.(in Chinese)
[20] 郭浩宇,张秀梅,张宗航,等.许氏平鲉仔、稚鱼的摄食特性及幼鱼胃排空率[J].水产学报,2017,41(2):285-296. GUO H Y,ZHANG X M,ZHANG Z H,et al.Study on feeding habits of Sebastes schlegelii larvae and gastric evacuation rate of juvenile[J].Journal of Fisheries of China,2017,41(2):285-296.(in Chinese)
[21] 张波,孙耀,唐启升.黑鲷的胃排空率[J].应用生态学报,2000,11(2):287-289. ZHANG B,SUN Y,TANG Q S.Gastric evacuation rate of black sea bream (Sparus macrocephalus)[J].Chinese Journal of Applied Ecology,2000,11(2):287-289.(in Chinese)
[22] 曲焕韬,廖建新,代伟,等.厚颌鲂幼鱼的胃排空特征及其数学模型研究[J].水产科学,2021,40(4):583-588. QU H T,LIAO J X,DAI W,et al.Analysis of gastric evacuation characteristics and associated mathematical models in juvenile Megalobrama pellegrini[J].Fisheries Science,2021,40(4):583-588.(in Chinese)
[23] 高云红,景琦琦,黄滨,等.云龙石斑鱼胃排空特征和摄食消化特性研究[J].渔业科学进展,2021,42(1):92-99. GAO Y H,JING Q Q,HUANG B,et al.Characteristics of gastric evacuation and feeding digestion in "Yunlong" groupers (Epinephelus moara ♀×E. lanceolatus)[J].Progress in Fishery Sciences,2021,42(1):92-99.(in Chinese)
[24] TEKINAY A A,GVNER Y.Effects of high dietary protein and high energy density on gastric evacuation and return of appetite in the rainbow trout,Oncorhynchus mykiss[J].Turkish Journal of Biology,2001,25(2):229-240.
[25] PÄÄKKÖNEN J P J,MARJOMÄKI T J.Gastric evacuation rate of burbot fed single-fish meals at different temperatures[J].Journal of Fish Biology,1997,50(3):555-563.

[26] HOPKINS T E,LARSON R J.Gastric evacuation of three food types in the black and yellow rockfish Sebastes chrysomelas (Jordan and Gilbert)[J].Journal of Fish Biology,1990,36(5):673-681.

[27] 曾令清,李凤杰,曹振东,等.南方鲇幼鱼的胃排空特征及其数学模型[J].水产学报,2011,35(2):231-237. ZENG L Q,LI F J,CAO Z D,et al.The analysis of gastric evacuation characteristics and associated mathematical models in juvenile southern catfish (Silurus meridionalis)[J].Journal of Fisheries of China,2011,35(2):231-237.(in Chinese)
[28] 邓乾龙, 王文, 董双林, 等. 大西洋鲑幼鱼适宜投喂频率和胃排空研究[J].中国海洋大学学报(自然科学版), 2022,52(03):52-60. DENG Q L,WANG W,DONG S L, et al.Studies on feeding frequency and gastric evacuation of juvenile altantic salmon(Salmo salar)[J].Periodical of Ocean University of China,2022,52(03):52-60.(in Chinese)
[29] 余方平,许文军,薛利建,等.美国红鱼的胃排空率[J].海洋渔业,2007,29(1):49-52. YU F P,XU W J,XUE L J,et al.The gastric evacuation rate of Sciaenops ocellatus[J].Marine Fisheries,2007,29(1):49-52.(in Chinese)
[30] SWENSON W A,SMITH L L,Jr.Gastric digestion,food consumption,feeding periodicity,and food conversion efficiency in walleye (Stizostedion vitreum vitreum)[J].Journal of the Fisheries Research Board of Canada,1973,30(9):1327-1336.

[31] 张涛,李伟杰,庄平,等.俄罗斯鲟幼鱼的胃排空特征及其数学模型[J].海洋渔业,2015,37(2):151-155. ZHANG T,LI W J,ZHUANG P.Analysis of gastric evacuation characteristics and associated mathematical models in juvenile Acipenser gueldenstaedtii[J].Marine Fisheries,2015,37(2):151-155.(in Chinese)
[32] RUGGERONE G T.Gastric evacuation rates and daily ration of piscivorous coho salmon,Oncorhynchus kisutch Walbaum[J].Journal of Fish Biology,1989,34(3):451-463.

[33] GROVE D J,LOIZIDES L G,NOTT J.Satiation amount,frequency of feeding and gastric emptying rate in Salmo gairdneri[J].Journal of Fish Biology,1978,12(5):507-516.

[34] RICHE M,HALEY D I,OETKER M,et al.Effect of feeding frequency on gastric evacuation and the return of appetite in tilapia Oreochromis niloticus (L.)[J].Aquaculture,2004,234(1/2/3/4):657-673.
[35] 张鹏飞,常青,陈四清,等.绿鳍马面鲀幼鱼昼夜摄食节律及胃排空模型研究[J].渔业科学进展,2020,41(1):104-111. ZHANG P F,CHANG Q,CHEN S Q,et al.Diet feeding rhythm and analysis of gastric evacuation associated mathematical models in juvenile Thamnaconus septentrionalis[J].Progress in Fishery Sciences,2020,41(1):104-111.(in Chinese)
[36] 朱云海,王跃斌,胡则辉,等.日本黄姑鱼幼鱼的胃排空率[J].海洋渔业,2013,35(4):448-452. ZHU Y H,WANG Y B,HU Z H,et al.Gastric evacuation of juvenile Nibea japonica[J].Marine Fisheries,2013,35(4):448-452.(in Chinese)
[37] 谢苏明,王裕玉,聂志娟,等.投喂频率对池塘工程化循环水养殖大口黑鲈生长、生理及肝脏GH、IGF-Ⅰ基因表达丰度的影响[J].中国水产科学,2020,27(4):363-374. XIE S M,WANG Y Y,NIE Z J,et al.Effects of feeding frequency on the growth,physiology and expression abundance of IGF-Ⅰ and GH mRNA in largemouth bass (Micropterus salmoides) reared in in-pond raceway culture systems[J].Journal of Fishery Sciences of China,2020,27(4):363-374.(in Chinese)
[38] 黄瑾,熊邦喜,陈洁,等.鱼类消化酶活性及其影响因素的研究进展[J].湖南农业科学,2011(5):129-131,141. HUANG J,XIONG B X,CHEN J,et al.Research advances in digestive enzyme of fish and its influencing factors[J].Hunan Agricultural Sciences,2011(5):129-131,141.(in Chinese)
[39] 尾崎久雄.鱼类消化生理[M].李爱杰,沈宗武,译.上海:上海科学技术出版社,1985. OZAKI H.Digestive physiology of fish[M].LI A J,SHEN Z W,translated.Shanghai:Shanghai Scientific & Technical Publishers,1985.(in Chinese)
[40] 杨家威,孙龙生,蔡春光,等.全雄黄颡鱼摄食前后消化酶活性变化规律的研究[J].饲料工业,2014,35(8):20-24. YANG J W,SUN L S,CAI C G,et al.Studies on changes of digestive enzyme activities after feeding in all-mall Pelteobagrus fulvidraco[J].Feed Industry,2014,35(8):20-24.(in Chinese)
[41] 翁祖桐.2个养殖水温下罗非鱼食后肠道消化酶活性的动态变化[J].渔业研究,2018,40(5):374-379. WENG Z T.The variations of digestive enzyme activities after feeding in intestinal canal of tilapia at two water temperatures[J].Journal of Fisheries Research,2018,40(5):374-379.(in Chinese)
[42] 周健,熊霞,李建中,等.乳源生物活性肽对动物肠道功能的影响及作用机制[J].动物营养学报,2016,28(7):1981-1987. ZHOU J,XIONG X,LI J Z,et al.Mechanisms and effects of milk-derived bioactive peptides on intestinal functions of animals[J].Chinese Journal of Animal Nutrition,2016,28(7):1981-1987.(in Chinese)
[43] KAIYA H,KOJIMA M,HOSODA H,et al.Peptide purification, complementary deoxyribonucleic acid (DNA) and genomic DNA cloning, and functional characterization of ghrelin in rainbow trout[J].Endocrinology,2003,144(12):5215-5226.

Options

Outlines

模态框（Modal）标题

Abstract

Cite this article

References