RESEARCH PAPER

Effects of Bioengineering Protein API on Growth Performance and Intestinal Health of Meat Rabbits

  • ZHANG Li ,
  • TANG Jiayong ,
  • JIA Gang ,
  • TIAN Gang ,
  • LIU Guangmang ,
  • CHEN Xiaoling ,
  • CAI Jingyi ,
  • ZHAO Hua
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  • Key Laboratory of Animal Disease-Resistant Nutrition of Sichuan Province, Key Laboratory of Animal Disease-Resistant Nutrition and Feed of Ministry of Agriculture and Rural Affairs, Key Laboratory of Animal Disease-Resistant Nutrition of Ministry of Education, Animal Nutrition Institute, Sichuan Agricultural University, Chengdu 611130, China

Received date: 2022-01-13

  Online published: 2022-07-14

Abstract

This experiment was conducted to evaluate the effects of supplementing bioengineering protein API (an engineered protein contained catfish antimicrobial peptide parasin I fragment) on growth performance and intestinal health of meat rabbits. A total of 144 healthy 30-day-old weaned meat rabbits with similar body weight[(644.93±31.92) g] were randomly assigned into 4 groups with 18 replicates per group and 2 rabbits per replicate. Rabbits in the control group were fed a basal diet, and others in experimental groups were fed basal diets supplemented with 0.1, 0.5 and 1.0 mg/kg API. The experiment lasted for 8 weeks. The results showed as follows:1) dietary API supplementation had no significant effects on the final weight, average daily feed intake (ADFI), average daily gain (ADG) and feed to weight ratio (F/G) of meat rabbits (P>0.05). Compared with the control group, dietary supplemented with 1.0 mg/kg API reduced the diarrhea rate, mortality rate and health risk index. 2) Compared with the control group, dietary supplemented with 1.0 mg/kg API significantly decreased the cecal Escherichia coli number (P<0.05), and significantly increased the cecal propionic acid ratio (P<0.05). 3) Compared with the control group, dietary supplemented with 1.0 mg/kg API significantly increased the jejunal Claudin-1 mRNA relative expression level (P<0.05), and dietary supplemented with 0.5 and 1.0 mg/kg API significantly increased the jejunal interleukin-2 (IL-2) mRNA relative expression level (P<0.05). 4) Compared with the control group, dietary supplemented with 1.0 mg/kg API significantly increased the jejunal amylase activity (P<0.05), and dietary supplemented with 0.1, 0.5 and 1.0 mg/kg API significantly increased the jejunal lipase and trypsin activities (P<0.05). In summary, dietary API supplementation reduces the diarrhea rate, mortality rate and cecal Escherichia coli number of meat rabbits, increases the mRNA relative expression levels of Claudin-1 and IL-2 and activity of digestive enzyme in jejunum, which shows API can improve the digestive capacity and intestinal barrier of the rabbits, then enhances the intestinal health. In general, the suitable dietary API supplemental level is 1.0 mg/kg in this experiment.

Cite this article

ZHANG Li , TANG Jiayong , JIA Gang , TIAN Gang , LIU Guangmang , CHEN Xiaoling , CAI Jingyi , ZHAO Hua . Effects of Bioengineering Protein API on Growth Performance and Intestinal Health of Meat Rabbits[J]. Chinese Journal of Animal Nutrition, 2022 , 34(7) : 4696 -4703 . DOI: 10.3969/j.issn.1006-267x.2022.07.056

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