Study on Expression Regularity of Important Proteins in Extracellular Matrix of Different Intestinal Segments during Weaning of 21-Day-Old Piglets

TANG Qingsong; WANG Li; XU E; LIU Chunyan; XIAO Mingfei; XIAO Hao; WU Qiwen; JIANG Zongyong; YI Hongbo

doi:10.3969/j.issn.1006-267x.2022.07.057

2022 , Vol. 34 >Issue 7: 4704 - 4715

DOI: https://doi.org/10.3969/j.issn.1006-267x.2022.07.057

RESEARCH PAPER

Study on Expression Regularity of Important Proteins in Extracellular Matrix of Different Intestinal Segments during Weaning of 21-Day-Old Piglets

TANG Qingsong ,
WANG Li ,
XU E ,
LIU Chunyan ,
XIAO Mingfei ,
XIAO Hao ,
WU Qiwen ,
JIANG Zongyong ,
YI Hongbo

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1. Guangdong Key Laboratory of Animal Breeding and Nutrition, Maoming Branch of Guangdong Laboratory for Lingnan Modern Agriculture Science and Technology, Key Laboratory of Animal Nutrition and Feed Science in South China, Ministry of Agriculture and Rural Affairs, State Key Laboratory of Livestock and Poultry Breeding, Institute of Animal Science, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China;
2. Institute of Animal Nutrition and Feed Science, College of Animal Science, Guizhou University, Guiyang 550025, China

Received date: 2022-01-08

Online published: 2022-07-14

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Abstract

The purpose of this experiment was to study the expression regularity of important proteins in the extracellular matrix (ECM) of different intestinal segments during weaning of 21-day-old piglets. Twenty-four piglets weaned at 21 days of age were randomly assigned into six pens. One pig was randomly selected from each pen at 0, 7, 14 and 28 days after weaned and executed after anesthesia, and then the samples of duodenum, jejunum and ileum were collected to determine the expression levels of ECM related genes and proteins. The results showed as follows:1) compared with 0 d, the mRNA relative expression levels of type Ⅰ collagen α2 chain (COL1A2), type Ⅲ collagen α1 chain (COL3A1), type Ⅳ collagen α2 chain (COL4A2), type Ⅴ collagen α1 chain (COL5A1), type Ⅵ collagen α1 chain (COL6A1), fibronectin 1 (FN1), tenascin C (TNC) and integrin α1 (ITGA1) in duodenum at 7, 14 and 28 d were significantly decreased (P<0.05). Moreover, the mRNA relative expression levels of COL1A2 and FN1 in duodenum were significantly decreased at 14 d compared with 28 d (P<0.05), and the COL3A1 mRNA relative expression level in duodenum was significantly lower at 14 d than that at 7 and 28 d (P<0.05), and the integrin β2 (ITGB2) mRNA relative expression level in duodenum at 7 d was lower than that at 14 and 28 d (P<0.05). 2) Compared with 0 d, the mRNA relative expression levels of COL1A2, COL3A1, COL4A2, and ITGA1 in jejunum at 7, 14 and 28 d were significantly decreased (P<0.05). Additionally, the mRNA relative expression levels of COL5A1 and COL6A1 in jejunum were significantly lower at 7 d than those at 0 and 28 d (P<0.05), and the mRNA relative expression levels of TNC and ITGB2 in jejunum at 7 d were significantly lower than that at 28 d (P<0.05). 3) The FN1 mRNA relative expression level in ileum at 7 d was significantly lower than that at 0, 14 and 28 d (P<0.05). 4) Compared with 0 d, the protein relative expression levels of COL1A2 and type Ⅵ collagen in jejunum at 7, 14 and 28 d were significantly decreased (P<0.05), and the protein relative expression levels of COL4A2, laminin and FN1 in jejunum at 14 and 28 d were significantly decreased (P<0.05). In conclusion, the expression and secretion of collagen (COL1A2, COL3A1, COL4A2, COL5A1, COL6A1 and type Ⅵ collagen), FN1, laminin, TNC, ITGA1 and ITGB2 are decreased in duodenum and jejunum of piglets after weaning; the mRNA relative expression levels of COL1A2, COL3A1 and FN1 in duodenum are the lowest at 14 d after weaning, and the mRNA relative expression levels of COL5A1 and COL6A1 in jejunum are the lowest at 7 d after weaning; the FN1 mRNA relative expression in ileum at 7 d after weaning is significantly decreased, but weaning has no significant effect on the expression of other ECM-related genes.

Key words： weaned piglets; extracellular matrix; collagen; expression regularity

Cite this article

TANG Qingsong , WANG Li , XU E , LIU Chunyan , XIAO Mingfei , XIAO Hao , WU Qiwen , JIANG Zongyong , YI Hongbo . Study on Expression Regularity of Important Proteins in Extracellular Matrix of Different Intestinal Segments during Weaning of 21-Day-Old Piglets[J]. Chinese Journal of Animal Nutrition, 2022 , 34(7) : 4704 -4715 . DOI: 10.3969/j.issn.1006-267x.2022.07.057

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