RESEARCH PAPER

Study on Digestion Extent of Miscellaneous Meal in Colon of Growing Pigs by Simulated Digestion

  • DU Zhongyuan ,
  • WANG Yuming ,
  • ZENG Shuli ,
  • GAO Lixiang ,
  • ZHAO Jiangtao ,
  • ZHANG Hu ,
  • ZHAO Feng
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  • 1. State Key Laboratory of Animal Nutrition, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China;
    2. Wen's Food Group Co., Ltd., Yunfu 527400, China

Received date: 2021-12-28

  Online published: 2022-07-14

Abstract

This experiment was conducted to investigate the difference between simulated and in vivo colonic digestion of miscellaneous meal for growing pigs, providing a reference for developing a simulated digestion method to predict the available energy of feed ingredients for growing pigs. A corn-soybean meal type diet was formulated as basal diet. Eighteen-point seventy-five percent of rapeseed meal, cottonseed meal, sunflower meal or peanut meal replaced was mixed with 81.25% of basal diet, respectively, to produce 4 experimental diets. Five barrows with the initial body weight of (39.4±2.9) kg surgically fitted with T-cannulas at the terminal cecum were allotted to 5 diets for digestion experiment in a 5×5 Latin square design with 5 periods. The terminal cecal digesta and feces were collected to determine the digestibility of dry matter and energy in anterior colon and total tract of experimental diets, then the digestibility of dry matter and energy of miscellaneous meals were calculated by difference method. A single-factor completely random design was adopted to study the simulated colonic digestion of growing pigs. Based on the simulated digestion system for monogastric animal, the simulated colonic fluid was prepared with the cecal freeze-dried powder (simulated digestion method 1) or simulated intestinal fluid powder (simulated digestion method 2), respectively. The colonic energy digestibility of terminal cecal digesta of experimental diets was evaluated by simulated digestion. Then, the colonic energy digestibility of miscellaneous meals was calculated according to the gastric-small intestine and gastric-small intestine-large (colon) simulated digestion. The results showed as follows:1) total tract energy digestibility of peanut meal was significantly higher than that of rapeseed meal, cottonseed meal and sunflower meal (P<0.05). No significant differences were observed in the energy digestibility and digestible energy (DE) in anterior colon and colon of 4 miscellaneous meals (P>0.05). The energy digestibility in anterior colon and colon accounted for 64.05% to 74.74% and 25.26% to 35.95% of total tract energy digestibility, respectively. 2) No significant differences were observed in the colonic energy digestibility and DE between simulated digestion method 1 and in vivo method for 4 miscellaneous meal diets (P>0.05). No significant differences were observed in the colonic energy digestibility and energetic value between simulated digestion method 2 and in vivo method for each of rapeseed meal, cottonseed meal, peanut meal corresponding diets (P>0.05), however, the significant difference was observed in sunflower meal and corresponding diet (P<0.05). It can be concluded that the difference in extent of digestion of simulated and in vivo colonic digestion of miscellaneous meal for growing pig is affected by source of simulated digestive enzymes.

Cite this article

DU Zhongyuan , WANG Yuming , ZENG Shuli , GAO Lixiang , ZHAO Jiangtao , ZHANG Hu , ZHAO Feng . Study on Digestion Extent of Miscellaneous Meal in Colon of Growing Pigs by Simulated Digestion[J]. Chinese Journal of Animal Nutrition, 2022 , 34(7) : 4716 -4725 . DOI: 10.3969/j.issn.1006-267x.2022.07.058

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