RESEARCH PAPER

Schizochytrium sp. Strain of Rich Docosahexaenoic Acid Mutagenized by Atmospheric and Room Temperature Plasma

  • HE Junhao ,
  • FU Zilin ,
  • DING Jun ,
  • MA Lu ,
  • BU Dengpan
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  • 1. State Key Laboratory of Animal Nutrition, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, China;
    2. Chinese Academy of Agricultural Sciences and World Agroforestry Center Joint Lab on Agroforestry and Sustainable Animal Husbandry, Beijing 100193, China

Received date: 2021-12-15

  Online published: 2022-07-14

Abstract

This study aimed to obtain a high docosahexaenoic acid (DHA)-production Schizochytrium sp. strain through mutation breeding, to provide the basis for developing dietary antibiotic alternatives to alleviate diarrhea occurred in calves, especially the calves before weaning. Schizochytrium sp. ATCC20888 as an original strain, which was mutagenized by atmospheric and room-temperature plasma. Mutagenized strains were selected by malonic acid, 2,2'-bipyridine and malonic acid-2,2'-bipyridine combination rapid screening protocols. The selected mutant strains were fermented in shake flasks, and then were evaluated for total oil and DHA production through vanillin phosphate detection and high-performance gas chromatography, to obtain high DHA production strains. The results showed that the average DHA production of Schizochytrium sp. strains screened by malonic acid, 2,2'-bipyridine and malonic acid-2,2'-bipyridine combination were all improved, which was improved by 32.20%, 25.01% and 29.71% compared with the original strain. The DHA content (54.56%) and production (10.88 g/L) of mutant strain I-F-9 screened by malonic acid-2,2'-bipyridine combination were improved by 74.62% and 61.17% compared with the original strain (P<0.05). In conclusion, the DHA content and production of Schizochytrium sp. strain I-F-9 mutagenized by atmospheric and room temperature plasma are significantly higher than the original strain, and the DHA production is relatively higher compared with other similar studies.

Cite this article

HE Junhao , FU Zilin , DING Jun , MA Lu , BU Dengpan . Schizochytrium sp. Strain of Rich Docosahexaenoic Acid Mutagenized by Atmospheric and Room Temperature Plasma[J]. Chinese Journal of Animal Nutrition, 2022 , 34(7) : 4750 -4760 . DOI: 10.3969/j.issn.1006-267x.2022.07.061

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