RESEARCH PAPER

Effects of Supplementing Fermented Yoghurt on Growth Performance, Nutrient Digestion and Fecal Flora of Weaned Piglets

  • CHEN Shenglei ,
  • LIN Ailian ,
  • YAN Xiaoxi ,
  • ZHU Chongmiao ,
  • SU Yong
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  • 1. Jiangsu Key Laboratory of Digestive Nutrition and Animal Health, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China;
    2. Nanjing Zhirun Biological Technology Group Co., Ltd., Nanjing 210095, China

Received date: 2021-12-26

  Online published: 2022-08-11

Abstract

This experiment was conducted to investigate the effects of supplementing fermented yoghurt on the growth performance, nutrient digestion and fecal flora of weaned piglets. Thirty-six 30-day-old weaned piglets with initial body weight of (9.83±0.07) kg were randomly divided into 3 groups with 6 replicates per group and 2 pigs per replicate. Piglets in the control group were fed a basal diet, those in the low-dose fermented yoghurt group were fed the basal diet supplemented with 40 mL yoghurt three times (07:00, 12:00 and 17:00) per day per pig, and those in the high-dose fermented yoghurt group were fed the basal diet supplemented with 80 mL yoghurt three times per day per pig. The experiment lasted for 14 days. The results showed as follows: 1) compared with the control group, the body weight of weaned piglets on day 14 and the average daily gain in days 8 to 14 in the high-dose fermented milk group were significantly increased (P<0.05), and the average daily gain in days 1 to 7 tended to be increased (P=0.058); the average daily feed intake of weaned piglets in days 8 to 14 in the low-dose fermented yogurt group and high-dose fermented yogurt group was significantly increased (P<0.05). 2) Compared with the control group, the serum albumin content of weaned piglets in the high-dose fermented yogurt group was significantly increased on day 7 (P<0.05), and the serum urea nitrogen content was significantly decreased on day 14 (P<0.05). 3) Compared with control group, the dry matter apparent digestibility of weaned piglets in the high-dose fermented yogurt group was significantly increased (P<0.05). 4) Compared with the control group, on day 3, the number of Salmonella and Escherichia coli in feces of weaned piglets in the low-dose fermented yogurt group was significantly decreased (P<0.05), while the number of lactic acid bacteria in feces of weaned piglets in the high-dose fermented yogurt group showed an increasing trend (P=0.08). 5) At the genus level, compared with the control group, the relative abundances of Collinsella and Mitsuokella in fecal flora of weaned piglets in the high-dose fermented yogurt group were significantly increased (P<0.05), while the relative abundances of Gemella, uncultured Carnobacteriaceae, Veillonella, Negativibacillus, Parasutterella and Rikenellaceae RC9 gut were significantly decreased (P<0.05). Meanwhile, the relative abundances of Enterorhabdus, Paludicola and Streptococcus in the high-dose fermented yogurt group and low-dose fermented yogurt group were significantly higher than those in the control group (P<0.05), and the Muribaculum relative abundance was significantly lower than that in the control group (P<0.05). The operational taxonomic unit (OTU) number in the high-dose fermented yogurt group was the largest, in which the [Eubacterium] oxidoreducens group and [Ruminococcus] gauvreauii group were the dominant genera. In conclusion, supplementing fermented yoghurt can improve the growth performance and dry matter apparent digestibility of weaned piglets, increase the relative abundances of beneficial bacteria such as Collinsella and Mitsuokella, and reduce the relative abundances of potentially pathogenic bacteria such as Veillonella and Parasutterella and the numbers of Salmonella and Escherichia coli in the feces on day 3 after the weaning.

Cite this article

CHEN Shenglei , LIN Ailian , YAN Xiaoxi , ZHU Chongmiao , SU Yong . Effects of Supplementing Fermented Yoghurt on Growth Performance, Nutrient Digestion and Fecal Flora of Weaned Piglets[J]. Chinese Journal of Animal Nutrition, 2022 , 34(8) : 4904 -4918 . DOI: 10.3969/j.issn.1006-267x.2022.08.017

References

[1] GRESSE R,CHAUCHEYRAS-DURAND F,FLEURY M A,et al.Gut microbiota dysbiosis in postweaning piglets:understanding the keys to health[J].Trends in Microbiology,2017,25(10):851-873.
[2] XIONG X,TAN B,SONG M,et al.Nutritional intervention for the intestinal development and health of weaned pigs[J].Frontiers in Veterinary Science,2019,6:46.
[3] ROGGERO P,LIOTTO N,POZZI C,et al.Analysis of immune,microbiota and metabolome maturation in infants in a clinical trial of Lactobacillus paracasei CBA L74-fermented formula[J].Nature Communications,2020,11(1):2703.
[4] SAKANDAR H A,ZHANG H P.Trends in probiotic (s)-fermented milks and their in vivo functionality:a review[J].Trends in Food Science&Technology,2021,110:55-65.
[5] CHEN Y J,MIN B J,CHO J H,et al.Effects of dietary bacillus-based probiotic on growth performance,nutrients digestibility,blood characteristics and fecal noxious gas content in finishing pigs[J].Asian-Australasian Journal of Animal Sciences,2006,19(4):587-592.
[6] 高擎燏,李平华,黄瑞华,等.猪源乳酸菌的抗逆性及益生性研究[J].畜牧与兽医,2015,47(10):41-46.GAO Q Y,LI P H,HUANG R H,et al.Study on stress resistance and beneficial nature of porcine lactic acid bacteria[J].Animal Husbandry&Veterinary Medicine,2015,47(10):41-46.(in Chinese)
[7] HUANG C H,QIAO S Y,LI D F,et al.Effects of lactobacilli on the performance,diarrhea incidence,VFA concentration and gastrointestinal microbial flora of weaning pigs[J].Asian-Australasian Journal of Animal Sciences,2004,17(3):401-409.
[8] HERES L,ENGEL B,URLINGS H A P,et al.Effect of acidified feed on susceptibility of broiler chickens to intestinal infection by Campylobacter and Salmonella[J].Veterinary Microbiology,2004,99(3/4):259-267.
[9] 栾海宏.复合型功能性寡糖对仔猪生长性能、肠道微生物菌群和免疫功能的影响[J].饲料研究,2021,44(5):29-32.LUAN H H.Effect of compound functional oligosaccharides on growth performance,intestinal microflora and immune function of piglets[J].Feed Research,2021,44(5):29-32.(in Chinese)
[10] MISSOTTEN J A M,MICHIELS J,GORIS J,et al.Screening of two probiotic products for use in fermented liquid feed[J].Livestock Science,2007,108(1/3):232-235.
[11] RATELIFFE B,COLE C B,FULLER R,et al.The effect of yoghurt and milk fermented with a porcine intestinal strain of Lactobacillus reuteri on the performance and gastrointestinal flora of pigs weaned at two days of age[J].Food Microbiology,1986,3(3):203-211.
[12] DAI Z L,ZHANG J,WU G Y,et al.Utilization of amino acids by bacteria from the pig small intestine[J].Amino Acids,2010,39(5):1201-1215.
[13] POLLMANN D.13-probiotics in pig diets[M]//HARESIGN W,COLE D J A.Recent advances in animal nutrition.Amsterdam:Elsevier Ltd.,1986:193-205.
[14] 徐秀景,谢长文,刘敏,等.乳酸菌发酵饲料对猪生长性能、肉品质和血液抗氧化指标的影响[J].中国饲料,2018(10):67-71.XU X J,XIE C W,LIU M,et al.Effects of Lactobacillus fermented feed on growth performance,meat quality and blood antioxidant capacity of pigs[J].China Feed,2018(10):67-71.(in Chinese)
[15] 张常明.乳酸菌对断奶仔猪生长性能和免疫功能影响的研究[D].硕士学位论文.广州:华南农业大学,2005.ZHANG C M.Effects of lactic acid bacteria on growth performance and immune function of weaned piglets[D].Master's Thesis.Guangzhou:South China Agricultural University,2005.(in Chinese)
[16] 陈鲜鑫,王金全,刘震坤,等.玉米乳杆菌发酵液体饲料对断奶仔猪生产性能、养分消化率(全收粪法)和血清生化免疫指标的影响[J].饲料研究,2017(5):5-9,39.CHEN X X,WANG J Q,LIU Z K,et al.Effects of Lactobacillus fermented liquid feed on performance,nutrient digestibility (total fecal collection method) and serum biochemical immune indices of weaned piglets[J].Feed Research,2017(5):5-9,39.(in Chinese)
[17] COLLINGTON G K,PARKER D S,ARMSTRONG D G.The influence of inclusion of either an antibiotic or a probiotic in the diet on the development of digestive enzyme activity in the pig[J].British Journal of Nutrition,1990,64(1):59-70.
[18] XUAN Z N,KIM J D,HEO K N,et al.Study on the development of a probiotics complex for weaned pigs[J].Asian-Australasian Journal of Animal Sciences,2001,14(10):1425-1428.
[19] KORNEGAY E T,RISLEY C R.Nutrient digestibilities of a corn-soybean meal diet as influenced by Bacillus products fed to finishing swine[J].Journal of Animal Science,1996,74(4):799-805.
[20] 王志祥,乔家运,王自恒,等.乳酸杆菌对断奶仔猪生长性能、养分表观消化率和消化酶活性的影响[J].西北农林科技大学学报(自然科学版),2006(4):23-27.WANG Z X,QIAO J Y,WANG Z H,et al.Effect of Lactobacillus on growth performance,nutrient digestibility and digestive enzyme activities of weaned piglets[J].Journal of Northwest A&F University (Natural Science Edition),2006(4):23-27.(in Chinese)
[21] 崔艳红,韩庆功,崔艺佳,等.益生菌复合发酵料对断奶仔猪消化环境、血清生化指标和代谢激素水平的影响[J].西北农业学报,2018,27(1):16-23.CUI Y H,HAN Q G,CUI Y J,et al.Effects of probiotics composite fermentation feed on gastrointestinal circumstance,serum biochemical and metabolic hormones indexes of weaning piglets[J].Acta Agriculturae Boreali-occidentalis Sinica,2018,27(1):16-23.(in Chinese)
[22] 杜泓明,汪晓东,王晓磊,等.不同益生菌培养物对断奶仔猪生长性能、免疫功能及血液生化指标的影响[J].中国兽医学报,2017,37(7):1379-1384,1400.DU H M,WANG X D,WANG X L,et al.Effect of different probiotic cultures on growth performance,immunological function and blood biochemical indexes of weaned piglets[J].Chinese Journal of Veterinary Science,2017,37(7):1379-1384,1400.(in Chinese)
[23] 刘辉,季海峰,王四新,等.复合乳酸菌发酵饲料对生长猪生长性能、粪便菌群、血清免疫和抗氧化指标的影响[J].动物营养学报,2022,34(2):783-794.LIU H,JI H F,WANG S X,et al.Effects of compound lactic acid bacteria fermented feed on growth performance,fecal microflora,serum immune and antioxidant indexes of growing pigs[J].Chinese Journal of Animal Nutrition,2022,34(2):783-794.(in Chinese)
[24] WANG S L,YAO B Q,GAO H,et al.Combined supplementation of Lactobacillus fermentum and Pediococcus acidilactici promoted growth performance,alleviated inflammation,and modulated intestinal microbiota in weaned pigs[J].BMC Veterinary Research,2019,15(1):239.
[25] 孙玉亭.芽孢乳杆菌S1生产工艺优化及其合生素对仔猪生长性能与粪样菌群的影响[D].硕士学位论文.南京:南京农业大学,2019.SUN Y T.Production process optimization of Sporolactobacillus S1 and its effect on growth performance and fecal flora of piglets[D].Master's Thesis.Nanjing:Nanjing Agricultural University,2019.(in Chinese)
[26] CASTILLO M,MARTÍN-ORÚE S M,MANZANILLA E G,et al.Quantification of total bacteria,enterobacteria and lactobacilli populations in pig digesta by real-time PCR[J].Veterinary Microbiology,2006,114(1/2):165-170.
[27] GIANG H H,VIET T Q,OGLE B,et al.Growth performance,digestibility,gut environment and health status in weaned piglets fed a diet supplemented with potentially probiotic complexes of lactic acid bacteria[J].Livestock Science,2010,129(1/2/3):95-103.
[28] PIEPER R,JANCZYK P,URUBSCHUROV V,et al.Effect of a single oral administration of Lactobacillus plantarum DSMZ 8862/8866 before and at the time point of weaning on intestinal microbial communities in piglets[J].International Journal of Food Microbiology,2009,130(3):227-232.
[29] 蔡艳,叶盛,韩晓云,等.乳酸菌对仔猪生长性能及肠道菌群的影响[J].江西畜牧兽医杂志,2018(5):15-16.CAI Y,YE S,HAN X Y,et al.Effects of lactic acid bacteria on growth performance and intestinal microflora of piglets[J].Jiangxi Journal of Animal Husbandry&Veterinary Medicine,2018(5):15-16.(in Chinese)
[30] 杜泓明.不同益生菌培养物对断奶仔猪生长性能、免疫功能及盲肠微生物区系的影响[D].硕士学位论文.长春:吉林农业大学,2017.DU H M.Effect of different probiotic cultures on growth performance,immune function and cecum microflora diversity of weaned piglets[D].Master's Thesis.Changchun:Jilin Agricultural University,2017.(in Chinese)
[31] 刘统.德氏乳杆菌对哺乳仔猪胃肠道微生物多样性影响研究[D].硕士学位论文.长沙:湖南农业大学,2012.LIU T.Effects of L.debrueckii on gastrointestinal microflora diversity of sucking piglets[D].Master's Thesis.Changsha:Hunan Agricultural University,2012.(in Chinese)
[32] DOS SANTOS T F,MELO T A,SANTOS D S,et al.Efficacy of oral administration of lactic acid bacteria isolated from cocoa in a fermented milk preparation:reduction of colitis in an experimental rat model[J].Genetics and Molecular Research,2016,15(3):gmr.15038097.
[33] JHA R,BERROCOSO J D.Review:dietary fiber utilization and its effects on physiological functions and gut health of swine[J].Animal,2015,9(9):1441-1452.
[34] 张丽.新型酵母培养物的制备及其对断奶仔猪生长性能、表观消化率和粪便微生物的影响[D].硕士学位论文.北京:中国农业科学院,2016.ZHANG L.The preparation of a novel yeast culture and its effect on growth performance,apparent nutrient digestibility and fecal microbiota of weaned piglets[D].Master's Thesis.Beijing:Chinese Academy of Agricultural Sciences,2016.(in Chinese)
[35] PARK S J,KIM J,LEE J S,et al.Characterization of the fecal microbiome in different swine groups by high-throughput sequencing[J].Anaerobe,2014,28:157-162.
[36] KIM H B,BOREWICZ K,WHITE B A,et al.Longitudinal investigation of the age-related bacterial diversity in the feces of commercial pigs[J].Veterinary Microbiology,2011,153(1/2):124-133.
[37] YU H,ZHOU T,GONG J H,et al.Isolation of deoxynivalenol-transforming bacteria from the chicken intestines using the approach of PCR-DGGE guided microbial selection[J].BMC Microbiology,2010,10:182.
[38] LAN G Q,ABDULLAH N,JALALUDIN S,et al.Purification and characterization of a phytase Mitsuokella jalaludinii,a bovine rumen bacterium[J].African Journal of Biotechnology,2011,10(59):12766-12776.
[39] BHATTI M A,FRANK M O.Veillonella parvula meningitis:case report and review of Veillonella infections[J].Clinical Infectious Diseases,2000,31(3):839-840.
[40] WANG Y,TAO H X,HUANG H M,et al.The dietary supplement Rhodiola crenulata extract alleviates dextran sulfate sodium-induced colitis in mice through anti-inflammation,mediating gut barrier integrity and reshaping the gut microbiome[J].Food&Function,2021,12(7):3142-3158.
[41] LIU Y Y,LI T,ALIM A,et al.Regulatory effects of stachyose on colonic and hepatic inflammation,gut microbiota dysbiosis,and peripheral CD4+ T cell distribution abnormality in high-fat diet-fed mice[J].Journal of Agricultural and Food Chemistry,2019,67(42):11665-11674.
[42] KIM H,PARK T,KWON I,et al.Specific inhibition of Streptococcus bovis by endolysin LyJH307 supplementation shifts the rumen microbiota and metabolic pathways related to carbohydrate metabolism[J].Journal of Animal Science and Biotechnology,2021,12(1):93.
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