RESEARCH PAPER

Effects of Mulberry Leaf Extract on Performance, Intestinal Immune Function, Intestinal Tissue Morphology and Cecal Microflora Structure of Laying Hens

  • ZHANG Bo ,
  • HUANG Chenxuan ,
  • CHEN Yifan ,
  • BAI Jingyu ,
  • WANG Dehe ,
  • CHEN Xiangyu ,
  • CHANG Dongmei ,
  • ZHANG Weiwei ,
  • MA Guodong ,
  • BAI Yuyu ,
  • LIU Yinyin ,
  • CHEN Hui ,
  • JI Ying
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  • 1. College of Animal Science and Technology, Agricultural University of Hebei, Baoding 071001, China;
    2. Regional Agricultural and Animal Husbandry Technology Extension Station, Tong Town, Jia County, Shaanxi Province, Jia County 719208, China;
    3. Zhengding County Mulberry Industry Application Research Institute, Zhengding 050800, China;
    4. Regional Agricultural and Animal Husbandry Technology Extension Station, Wangjiabian Town, Jia County, Shaanxi Province, Jia County 719208, China;
    5. College of Information Science and Technology, Agricultural University of Hebei, Baoding 071001, China

Received date: 2021-12-21

  Online published: 2022-08-11

Abstract

The purpose of this experiment was conducted to study the effects of mulberry leaf extract on the performance, intestinal immune function, intestinal tissue morphology and cecal microflora structure of laying hens. Two hundred and eighty-eight 38-week-old Roman gray laying hens were randomly divided into 4 groups with 6 replicates per group and 12 hens per replicate. The pre-feeding period lasted for 14 days, and the formal period lasted for 56 days. During the pre-feeding period, all hens were fed a basal diet. During the formal period, hens in the control group were fed the basal diet, while those in the experimental groups were fed the basal diet supplemented with 0.4%, 0.8% and 1.2% mulberry leaf extract, respectively. The results showed as follows: 1) compared with the control group, diet supplemented with 0.4%, 0.8% or 1.2% mulberry leaf extract had no significant effect on the performance of laying hens (P>0.05). 2) Compared with the control group, diet supplemented with 0.4%, 0.8% or 1.2% mulberry leaf extract had a significant effect on the interleukin (IL-4) content in ileal mucosa (P<0.05). 3) Compared with the control group, diet supplemented with 0.4%, 0.8% or 1.2% mulberry leaf extract significantly increased the villus height of duodenum, jejunum and ileum and the ratio of villi height to crypt depth of ileum (P<0.05); diet supplemented with 1.2% mulberry leaf extract significantly reduced the crypt depth of jejunum (P<0.05); diet supplemented with 0.4% or 1.2% mulberry leaf extracts significantly reduced the crypt depth of ileum (P<0.05). 4) Compared with the control group, diet supplemented with 0.4%, 0.8% or 1.2% mulberry leaf extract significantly reduced the relative abundance of the Proteobacteria in cecal microflora (P<0.05); diet supplemented with 0.4% or 1.2% mulberry leaf extract significantly increased the relative abundance of Faecalibacterium in cecal microflora (P<0.05); diet supplemented with 1.2% mulberry leaf extract significantly increased the relative abundance of Phascolarctobacterium in cecal microflora (P<0.05). In conclusion, adding mulberry leaf extracts into the diet can improve the intestinal immune function, improve the intestinal tissue morphology and the structure of cecal microflora. Diet supplemented with 0.8% mulberry leaf extracts is the most appropriate diet for laying hens under this experimental condition.

Cite this article

ZHANG Bo , HUANG Chenxuan , CHEN Yifan , BAI Jingyu , WANG Dehe , CHEN Xiangyu , CHANG Dongmei , ZHANG Weiwei , MA Guodong , BAI Yuyu , LIU Yinyin , CHEN Hui , JI Ying . Effects of Mulberry Leaf Extract on Performance, Intestinal Immune Function, Intestinal Tissue Morphology and Cecal Microflora Structure of Laying Hens[J]. Chinese Journal of Animal Nutrition, 2022 , 34(8) : 4977 -4987 . DOI: 10.3969/j.issn.1006-267x.2022.08.023

References

[1] CAI M,MU L,WANG Z L,et al.Assessment of mulberry leaf as a potential feed supplement for animal feeding in P.R.China[J].Asian-Australasian Journal of Animal Sciences,2019,32(8):1145-1152.
[2] HE X R,FANG J C,RUAN Y L,et al.Structures,bioactivities and future prospective of polysaccharides from Morus alba(white mulberry):a review[J].Food Chemistry,2018,245:899-910.
[3] 陶程.广西现行桑树品种枝条总生物碱、多糖、总黄酮含量的研究[D].硕士学位论文.南宁:广西大学,2018.TAO C.Study on total alkaloid,polysaccharide and total flavonoids content in branches of mulberry species in Guangxi[D].Master's Thesis.Nanning:Guangxi University,2018.(in Chinese)
[4] 杨灿,唐小武,欧慧敏,等.桑叶的营养价值及其在畜禽生产中的应用研究进展[J].中国畜牧兽医,2019,46(5):1388-1396.YANG C,TANG X W,OU H M,et al.Research progress on nutritional value of mulberry leaf and its application in animal production[J].China Animal Husbandry&Veterinary Medicine,2019,46(5):1388-1396.(in Chinese)
[5] LI J S,JI T,SU S L,et al.Mulberry leaves ameliorate diabetes via regulating metabolic profiling and AGEs/RAGE and p38 MAPK/NF-κB pathway[J].Journal of Ethnopharmacology,2022,283:114713.
[6] 章学东,李有贵,张雷,等.桑叶粉对蛋鸡生产性能、蛋品质和血清生化指标的影响研究[J].中国家禽,2012,34(16):25-28.ZHANG X D,LI Y G,ZHANG L,et al.Effect of dietary mulberry leaves on productive performance,egg quality and serum biochemical indices of laying hens[J].China Poultry,2012,34(16):25-28.(in Chinese)
[7] 邱时秀,雷春龙,李娟,等.桑叶粉对蛋鸡生产性能和蛋品质的影响[J].黑龙江畜牧兽医,2017(22):184-186.QIU S X,LEI C L,LI J,et al.The effect of mulberry leaf powder on the production performance and egg quality of laying hens[J].Heilongjiang Animal Science and Veterinary Medicine,2017(22):184-186.(in Chinese)
[8] 邱时秀,李娟,陈亚迎,等.桑枝叶干粉对彭县黄鸡生长性能、屠宰性能及抗氧化、免疫功能的影响[J].黑龙江畜牧兽医,2019(10):111-114.QIU S X,LI J,CHEN Y Y,et al.Effects of dry powder of mulberry branches and leaves on growth performance,slaughter performance,antioxidant and immune functions of Pengxian yellow chickens[J].Heilongjiang Animal Science and Veterinary Medicine,2019(10):111-114.(in Chinese)
[9] 邝哲师,黄静,廖森泰,等.桑叶粉和发酵桑叶粉对胡须鸡屠宰性能、肉品质及盲肠菌群的影响[J].中国畜牧兽医,2016,43(8):1989-1997.KUANG Z S,HUANG J,LIAO S T,et al.Effects of mulberry leaf meal and fermented mulberry leaf meal on slaughter performance,meat quality and cecum microflora in Huxu chicken[J].China Animal Husbandry&Veterinary Medicine,2016,43(8):1989-1997.(in Chinese)
[10] ZHAO X J,LI L,LUO Q L,et al.Effects of mulberry (Morus alba L.) leaf polysaccharides on growth performance,diarrhea,blood parameters,and gut microbiota of early-weanling pigs[J].Livestock Science,2015,177:88-94.
[11] 呼红梅,郝丽红,王怀中,等.发酵桑叶对生长育肥猪生长性能、胴体品质和肌肉营养成分的影响[J].动物营养学报,2021,33(11):6104-6113.HU H M,HAO L H,WANG H Z,et al.Effects of fermented mulberry leaves on growth performance,carcass quality and muscle nutrients of growing-finishing pigs[J].Chinese Journal of Animal Nutrition,2021,33(11):6104-6113.(in Chinese)
[12] 雷春龙,李娟,吴永胜,等.桑枝叶提取物对蛋鸡生产性能、血清免疫指标与抗氧化能力的影响[J].中国畜牧杂志,2019,55(8):118-122.LEI C L,LI J,WU Y S,et al.Effects of mulberry branch and leaf extract on growth performance,serum immune indexes and antioxidant capacity of laying hens[J].Chinese Journal of Animal Science,2019,55(8):118-122.(in Chinese)
[13] 张波,陈祥宇,王德贺,等.桑叶的营养价值及在动物生产中的应用[J].饲料研究,2021,44(7):143-145.ZHANG B,CHEN X Y,WANG D H,et al.Nutritional value of mulberry leaves and its application in animal production[J].Feed Research,2021,44(7):143-145.(in Chinese)
[14] 张霞.PD-1/PD-Ls在IgG4-RD中作用的研究及过敏/嗜酸性粒细胞升高在IgG4-RD中的意义[D].博士学位论文.北京:北京协和医学院,2019.ZHANG X.The role of PD-1/PD-Ls in IgG4-RD and the significance of hypersensitivity/eosinophilia in IgG4-RD[D].Ph.D.Thesis.Beijing:Peking Union Medical College,2019.(in Chinese)
[15] 杨春林.自然杀伤细胞及其亚型在实验性自身免疫性重症肌无力中的免疫调节作用[D].博士学位论文.济南:山东大学,2020.YANG C L.Immune-regulation of natural killer cells and their subtypes on experimental autoimmune myasthenia gravis[D].Ph.D.Thesis.Jinan:Shandong University,2020.(in Chinese)
[16] CHEN X L,SHENG Z C,QIU S L,et al.Purification,characterization and in vitro and in vivo immune enhancement of polysaccharides from mulberry leaves[J].PLoS One,2019,14(1):e0208611.
[17] 冯麒凤,李战福,黄先智,等.日粮中添加桑叶提取物和1-脱氧野尻霉素对大鲵生长、消化、免疫能力和肠道菌群的影响[J].水生生物学报,2021,45(3):582-592.FENG Q F,LI Z F,HUANG X Z,et al.Effects of dietary mulberry leaf extract and 1-deoxynojirimycin on growth,digestion and immunity capacity,and intestinal microorganism of Chinese giant salamander (Andrias davidianus)[J].Acta Hydrobiologica Sinica,2021,45(3):582-592.(in Chinese)
[18] 许祯莹,雷春龙,李娟,等.日粮中添加桑叶粉对蛋鸡血液抗氧化和免疫指标的影响[J].畜牧与兽医,2017,49(12):34-38.XU Z Y,LEI C L,LI J,et al.Effects of mulberry leaf meal on serum antioxidant indexes and immune indexes of laying hens[J].Animal Husbandry&Veterinary Medicine,2017,49(12):34-38.(in Chinese)
[19] AI J,BAO B,BATTINO M,et al.Recent advances on bioactive polysaccharides from mulberry[J].Food&Function,2021,12(12):5219-5235.
[20] 黄世荣,洪德志,李晓童,等.桑枝多糖对免疫抑制小鼠的免疫调节作用[J].蚕业科学,2017,43(3):467-471.HUANG S R,HONG D Z,LI X T,et al.Immunoregulatory functions of mulberry branch polysaccharides on immunosuppressed mice[J].Acta Sericologica Sinica,2017,43(3):467-471.(in Chinese)
[21] 洪德志,张作法,蒋学,等.桑枝多糖对正常小鼠免疫功能的影响[J].蚕业科学,2011,37(3):481-485.HONG D Z,ZHANG Z F,JIANG X,et al.Effects of polysaccharide from Ramulus mori on immunologic functions of normal mouse[J].Acta Sericologica Sinica,2011,37(3):481-485.(in Chinese)
[22] ABDELATTY A M,MANDOUH M I,MOHAMED S A,et al.Azolla leaf meal at 5% of the diet improves growth performance,intestinal morphology and p70S6K1 activation,and affects cecal microbiota in broiler chicken[J].Animal,2021,15(10):100362.
[23] SHI H T,ZHAO S Z,WANG K L,et al.Effects of dietary Astragalus membranaceus supplementation on growth performance,and intestinal morphology,microbiota and metabolism in common carp (Cyprinus carpio)[J].Aquaculture Reports,2022,22:100955.
[24] TEJEDA O J,KIM W K.Effects of fiber type,particle size,and inclusion level on the growth performance,digestive organ growth,intestinal morphology,intestinal viscosity,and gene expression of broilers[J].Poultry Science,2021,100(10):101397.
[25] 徐静,张子儒,王德贺,等.饮水中添加大蒜精油对蛋鸡生长性能、肠道组织形态及盲肠菌群的影响[J].动物营养学报,2021,33(1):308-316.XU J,ZHANG Z R,WANG D H,et al.Effects of adding garlic essential oil into drinking water on growth performance,intestinal tissue morphology and cecum microbial flora of layer hens[J].Chinese Journal of Animal Nutrition,2021,33(1):308-316.(in Chinese)
[26] 王鑫鑫,李茜茜,吕毅,等.组合蛋白酶对肉仔鸡采食偏好性、血清生化指标和肠道组织形态的影响[J].动物营养学报,2022,34(1):264-273.WANG X X,LI X X,LYU Y,et al.Effects of combinatorial protease on feed intake preference,serum biochemical indices and intestinal tissue morphology of broilers[J].Chinese Journal of Animal Nutrition,2022,34(1):264-273.(in Chinese)
[27] ZHAO Y Z,FU J H,LI P,et al.Effects of dietary glucose oxidase on growth performance and intestinal health of AA broilers challenged by Clostridium perfringens[J].Poultry Science,2022,101(1):101553.
[28] XIE Y H,WANG L X,SUN H,et al.Immunomodulatory,antioxidant and intestinal morphology-regulating activities of alfalfa polysaccharides in mice[J].International Journal of Biological Macromolecules,2019,133:1107-1114.
[29] 张金龙,张宁,杨雪,等.发酵桑叶粉对肉鸡生长性能、血清生化指标及肠道组织形态结构的影响[J].饲料工业,2017,38(20):38-42.ZHANG J L,ZHANG N,YANG X,et al.Effects of fermented product of mulberry leaf on growth performance,serum biochemical indexes and intestinal morphology of broiler chickens[J].Feed Industry,2017,38(20):38-42.(in Chinese)
[30] WANG F,LI J R,JIANG Y M.Polysaccharides from mulberry leaf in relation to their antioxidant activity and antibacterial ability[J].Journal of Food Process Engineering,2010,33(1):39-50.
[31] 谢红兵.植物多糖对断奶仔猪肠道生理特征和免疫机能的影响及机理研究[D].博士学位论文.长沙:湖南农业大学,2018.XIE H B.Effects and mechanism of botanical polysaccharides on intestinal physiological characteristics and immue function of weaned piglets[D].Ph.D.Thesis.Changsha:Hunan Agricultural University,2018.(in Chinese)
[32] SHREINER A B,KAO J Y,YOUNG V B.The gut microbiome in health and in disease[J].Current Opinion in Gastroenterology,2015,31(1):69-75.
[33] YIN C M,NORATTO G D,FAN X Z,et al.The impact of mushroom polysaccharides on gut microbiota and its beneficial effects to host:a review[J].Carbohydrate Polymers,2020,250:116942.
[34] 李玲,孙小红,连旭,等.凉茶渣替代象草对育肥牛空肠组织形态、屏障功能以及菌群结构的影响[J].动物营养学报,2022,34(2):1087-1097.LI L,SUN X H,LIAN X,et al.Effects of herbal tea residue on ileum morphology,barrier function and flora structure of fattening cattle[J].Chinese Journal of Animal Nutrition,2022,34(2):1087-1097.(in Chinese)
[35] TIAN H C,LIANG Y,LIU G B,et al.Moringa oleifera polysaccharides regulates caecal microbiota and small intestinal metabolic profile in C57BL/6 mice[J].International Journal of Biological Macromolecules,2021,182:595-611.
[36] ZHAO R Q,HU Q H,MA G X,et al.Effects of Flammulina velutipes polysaccharide on immune response and intestinal microbiota in mice[J].Journal of Functional Foods,2019,56:255-264.
[37] 程冉冉.粪菌移植对鸡生长发育及肠道菌群的影响[D].硕士学位论文.武汉:华中农业大学,2020.CHENG R R.The effect of fecal microbiota transplantation on growth performance and gut microbiota in chickens[D].Master's Thesis.Wuhan:Huazhong Agricultural University,2020.(in Chinese)
[38] 王文文,王园,孟子琪,等.发酵麸皮多糖对杜寒杂交肉羊瘤胃菌群及其屏障功能相关指标的影响[J].动物营养学报,2022,34(1):640-650.WANG W W,WANG Y,MENG Z Q,et al.Effects of fermented wheat bran polysaccharides on microbiota and rumen barrier junction related indices of Dorper×thin-tailed Han crossbred meat lambs[J].Chinese Journal of Animal Nutrition,2022,34(1):640-650.(in Chinese)
[39] 孙悦,戴求仲,蒋桂韬,等.饲粮支链氨基酸比例对28~63日龄攸县麻鸭肠道菌群的影响[J/OL].广西师范大学学报(自然科学版):1-10.(2021-03-29).https://kns.cnki.net/kcms/detail/detail.aspx?FileName=GXSF20210304001&DbName=CAPJ2021.DOI:10.16088/j.issn.1001-6600.2020110901. SUN Y,DAI Q Z,JIANG G T,et al.Effect of dietary branched-chain amino acid ratio on intestinal flora of 28-63 days old Youxian duck[J/OL].Journal of Guangxi Normal University (Natural Science Edition):1-10.(2021-03-29).https://kns.cnki.net/kcms/detail/detail.aspx?FileName=GXSF20210304001&DbName=CAPJ2021.DOI:10.16088/j.issn.1001-6600.2020110901. (in Chinese)
[40] LAURSEN M F,LAURSEN R P,LARNKJÆR A,et al.Faecalibacterium gut colonization is accelerated by presence of older siblings[J].mSphere,2017,2(6):e00448-17.
[41] 李瑞萍,刘长国,张硕,等.蝉花多糖对肉鸡生长性能、抗氧化能力和肠道菌群的影响[J].中国兽医学报,2021,41(6):1118-1126.LI R P,LIU C G,ZHANG S,et al.Effects of Cordyceps cicadae polysaccharide on growth performance,antioxidant capacity and intestinal microbial diversity of broiler chickens[J].Chinese Journal of Veterinary Science,2021,41(6):1118-1126.(in Chinese)
[42] HOU D W,HUANG Z J,ZENG S Z,et al.Intestinal bacterial signatures of white feces syndrome in shrimp[J].Applied Microbiology and Biotechnology,2018,102(8):3701-3709.
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