Effects of Dietary Crude Protein Level on Digestive Enzyme Activities, Metabolism of Energy and Protein of Broilers

YE Xiaomeng; SONG Mingqiang; SUN Xiaoxiao; WANG Yuming; XIE Jingjing; SA Renna; ZHANG Hu; ZHAO Feng

doi:10.3969/j.issn.1006-267x.2022.08.024

Chinese Journal of Animal Nutrition >

2022 , Vol. 34 >Issue 8: 4988 - 4999

DOI: https://doi.org/10.3969/j.issn.1006-267x.2022.08.024

RESEARCH PAPER

Effects of Dietary Crude Protein Level on Digestive Enzyme Activities, Metabolism of Energy and Protein of Broilers

YE Xiaomeng ,
SONG Mingqiang ,
SUN Xiaoxiao ,
WANG Yuming ,
XIE Jingjing ,
SA Renna ,
ZHANG Hu ,
ZHAO Feng

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The State Key Laboratory of Animal Nutrition, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China

Received date: 2022-01-20

Online published: 2022-08-11

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Abstract

This experiment was conducted to investigate the effects of dietary crude protein (CP) level on growth performance and digestion function of broilers, and to make sure whether it caused differences in the determination of apparent metabolizable energy (AME) of diets. Two trials were included in this experiment. Trial 1 was to investigate the effects of dietary CP level on growth performance and digestive enzyme activities of broilers. Two CP level diets consisted of high CP level (HP) diet (22.57% CP for days 8 to 21 and 20.65% CP for days 22 to 35) and low CP level (LP) diet (19.29% CP for days 8 to 21 and 17.49% CP for days 22 to 35) were used in a completely randomized block design according to the body weight at 7 days of age. Each treatment consisted of 6 replicates of 24 broilers from 8 to 21 days of age or 17 broilers from 22 to 35 days of age. Trial 2 was to investigate the effects of broilers fed diets contained different CP levels on digestible nutrients in excreta and metabolism of energy and protein of diets. The two sources of broilers fed HP diets or LP diets and two diets of HP diets (22.57% CP for days 21 to 28 and 20.65% CP for days 35 to 45) or LP diets (19.29% CP for days 21 to 28 and 17.49% CP for days 35 to 42) were used in a 2×2 two-factor completely randomized design. Each treatment consisted of 6 replicates of 4 broilers from 21 to 28 days of age or 2 broilers from 35 to 42 days of age. The results showed as follows: 1) the average daily gain (ADG) of 8 to 21 days of age was significantly higher of broilers fed LP diet than HP diet (P<0.05), but no significant difference was observed in ADG of 22 to 35 days of age of broilers fed two CP level diets (P>0.05). 2) The activities of amylase and chymotrypsin in jejunum fluid and the activity of amylase in ileal fluid on day 21 of broilers fed HP diet were significantly increased (P<0.05), and the activity of amylase in ileum fluid on day 35 of broilers fed HP diet was significantly increased compared with these of broliers fed LP diet (P<0.05). 3) From 21 to 28 days of age, enzymatic hydrolystae gross energy (EHGE) of excreta per kg dietary intake (EHGE/DMI) and EHGE of excrta per MJ intake (EHGE/GEI) of broilers fed HP diet had no significant differences compared with these of broliers fed LP diet (P<0.05). 4) Broilers fed diets contained different CP levels from 25 to 28 days of age or 39 to 42 days of age had no significant effects on AME, nitrogen-corrected apparent metabolizable energy (AMEn), AME/GE, AMEn/GE and CP metabolizability of diets (P>0.05). In conclusion, although dietary CP level affects ADG and the activities of some digestive enzymes in digestive fluid of broilers, it has no significant effect on the determination of dietary AME and CP metabolizability by biological method.

Key words： protein level; chicken; growth performance; digestive enzyme; metabolizable energy

Cite this article

YE Xiaomeng , SONG Mingqiang , SUN Xiaoxiao , WANG Yuming , XIE Jingjing , SA Renna , ZHANG Hu , ZHAO Feng . Effects of Dietary Crude Protein Level on Digestive Enzyme Activities, Metabolism of Energy and Protein of Broilers[J]. Chinese Journal of Animal Nutrition, 2022 , 34(8) : 4988 -4999 . DOI: 10.3969/j.issn.1006-267x.2022.08.024

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