The purpose of this study was to investigate the effects of conjugated linoleic acid (CLA) on performance, egg quality, serum and yolk biochemical indices, antioxidant capacity and enrichment in egg yolk of royal chickens, in order to develop CLA-enhanced eggs. A total of 360 healthy royal chickens of 63-week-old were randomly divided into 5 groups with 6 replicates per group and 12 chickens per replicate. Chickens in the control group were fed a basal diet+4% soybean oil, and those in CLA groups were fed diets supplemented with 1%, 2%, 3% and 4% CLA on the basis of the control group, respectively, and soybean oil was replaced with the corresponding amount of CLA, respectively. The pre-trial period lasted for 7 days, and the trial period lasted for 42 days. The results showed as follows: 1) compared with the control group, the average daily feed intake of royal chickens in 1% CLA group and 4% CLA group was extremely significantly decreased (P<0.01), the laying rate in 4% CLA group was significantly decreased (P<0.05), and the feed to egg ratio in 1% CLA group, 2% CLA group and 3% CLA group was significantly increased (P<0.05). 2) Compared with the control group, dietary CLA tended to increase the eggshell strength of royal chickens (P=0.060). 3) Compared with the control group, the serum triglyceride (TG) content of royal chickens in 2% CLA group, 3% CLA group and 4% CLA group was extremely significantly decreased (P<0.01), the serum low density lipoprotein cholesterol (LDL-C) content in CLA groups was extremely significantly decreased (P<0.01), and the serum high density lipoprotein cholesterol (HDL-C) content in 2% CLA group was extremely significantly increased (P<0.01); the egg yolk total cholesterol content of royal chickens in CLA groups was extremely significantly decreased (P<0.01). 4) Compared with the control group, the serum superoxide dismutase (SOD) activity of royal chickens in 1% CLA group was extremely significantly increased (P<0.01), the serum glutathione peroxidase (GSH-Px) activity in 4% CLA group was extremely significantly increased (P<0.01), the serum malondialdehyde (MDA) content in CLA groups was extremely significantly decreased (P<0.01), and the serum catalase (CAT) activity in 2% CLA group and 3% CLA group was extremely significantly increased (P<0.01); the total superoxide capacity (T-AOC) and the activities of SOD and CAT in egg yolk of royal chickens in 2% CLA group, 3% CLA group and 4% CLA group were extremely significantly increased (P<0.01), the GSH-Px activity in egg yolk in all CLA groups was extremely significantly increased (P<0.01), and the MDA content in egg yolk was extremely significantly decreased (P<0.01). 5) Compared with the control group, the CLA enrichment in 2% CLA group was extremely significantly increased (P<0.01). In conclusion, diets supplemented with 3% and 4% CLA have adverse effects on performance of royal chickens; dietary 2% CLA can increase the eggshell strength, decrease the cholesterol content in serum and yolk, improve antioxidant capacity, and the CLA enrichment in yolk is the highest, which is conducive to the production of CLA-enhanced eggs.
CHANG Qiangqiang
,
GUO Yan
,
SUN Tianyuan
,
LIU Xiaoxia
,
LI Yali
,
ZHANG Meng
,
YANG Yu
,
WANG Xuzhen
,
HAO Songhua
,
ZHAO Yali
,
YANG Zisen
,
ZHANG Junzhen
. Effects of Conjugated Linoleic Acid on Performance, Egg Quality, Serum and Egg Yolk Biochemical Indices and Enrichment in Egg Yolk of Royal Chickens[J]. Chinese Journal of Animal Nutrition, 2022
, 34(8)
: 5039
-5047
.
DOI: 10.3969/j.issn.1006-267x.2022.08.028
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