Chinese Journal of Animal Nutrition >

2022 , Vol. 34 >Issue 8: 5048 - 5062

DOI: https://doi.org/10.3969/j.issn.1006-267x.2022.08.029

RESEARCH PAPER

Effects of Turpiniae folium Extract on Growth Performance, Slaughter Performance, Serum Immune and Antioxidant Function and Intestinal Microecology in Wenchang Chicken under Hot-Humid Conditions

  • SONG Wenjing ,
  • SONG Qiongli ,
  • CHEN Xiaolian ,
  • LIU Linxiu ,
  • TAN Jia ,
  • ZOU Zhiheng ,
  • WEI Limin ,
  • XIONG Pingwen ,
  • ZHANG Yan ,
  • SUN Ruiping
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  • 1. Institute of Animal Husbandry and Veterinary Science, Jiangxi Academy of Agricultural Sciences, Nanchang 330200, China;
    2. Institute of Animal Science and Veterinary Medicine, Hainan Academy of Agricultural Sciences, Haikou 571100;
    3. Key Laboratory of Tropical Animal Breeding and Disease Research, Haikou 570203, China

Received date: 2022-01-10

  Online published: 2022-08-11

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Abstract

The purpose of this study was to evaluate the effects of Turpiniae folium extract (TFE) supplementation on the growth performance, slaughter performance, serum immune and antioxidant function and intestinal microecology of Wenchang chicken under hot-humid condition in summer. A total 240 female Wenchang chicken at 84 days of age with similar body weight were randomly divided into 4 groups, and each group included 6 replicates with 10 birds per replicate. Birds received either a basal diet (control group) or the basal diet+0.20%, 0.40% or 0.80% TFE. The experiment period lasted for 35 days. Throughout the experiment period, birds were under hot-humid condition (average daily temperature was 28.26 to 34.80 ℃, average daily relative humidity was 67.00% to 87.58%). The results showed as follows: 1) with the TFE supplementation level increasing, the average daily gain (ADG) linearly (P=0.004) and quadratically (P=0.001) changed. Compared with the control group, feed to gain ratio (F/G) tended to decrease (0.05≤P<0.10). 2) Compared with the control group, serum total protein (TP), immunoglobulin M (IgM) and insulin (INS) contents tended to increase (0.05≤P<0.10). With the TFE supplementation level increasing, serum interleukin-10 (IL-10) content linearly (P=0.002) and quadratically (P=0.006) changed, but serum interleukin-1β (IL-1β) and interleukin 6 (IL-6) contents linearly (P=0.004, P=0.005) and quadratically (P=0.009, P=0.005) changed. Compared with the control group, dietary supplementation with 0.04% and 0.08% TFE significantly decreased the serum glucose (GLU), tumor necrosis factor-α (TNF-α), heat stress protein 70 (HSP70) and malondialdehyde (MDA) contents (P＜0.05), but significantly increased the contents of serum immunoglobulin A (IgA), immunoglobulin G (IgG), interleukin 10 (IL-10), total antioxidant capacity (T-AOC), and activities of superoxide dismutase (SOD) and peroxidase (GSH-Px) (P<0.05). 3) With the TFE supplementation level increasing, spleen index quadratically changed (P=0.043). 4) Jejunum villus height (VH) and villus height/crypt depth (VH/CD) in 0.04% and 0.08% TFE groups were significantly higher than those in the control group (P<0.05). 5) With the TFE supplementation level increasing, the relative abundance of Proteobacteria linearly (P=0.016) and quadratically (P=0.011) changed. Compared with the control group, the relative abundance of Bifidobacterium tended to increased (0.05≤P<0.10). Compared with the control group, adding 0.04% and 0.08% TFE significantly decreased the relative abundance of Fusobacteria and Prevotellaceae_UCG-001 (P<0.05), and adding 0.08% TFE significantly increased the relative abundance of Firmicutes and Lactobacillus (P<0.05). In conclusion, TFE supplementation improves the growth performance, immune and antioxidant fuction of Wenchang chicken, alleviates inflammatory reaction, improves intestinal mucosal morphology and regulates intestinal microflora. According to the quadratic regression analyses fitted to significance parameters, the optimal dietary TFE supplementation level is 0.067%.

Key words： Turpinia folium extract; hot-humid condition; Wenchang chicken; growth performance; slaughter performance; immune and antioxidant function; intestinal microecology

Cite this article

SONG Wenjing , SONG Qiongli , CHEN Xiaolian , LIU Linxiu , TAN Jia , ZOU Zhiheng , WEI Limin , XIONG Pingwen , ZHANG Yan , SUN Ruiping . Effects of Turpiniae folium Extract on Growth Performance, Slaughter Performance, Serum Immune and Antioxidant Function and Intestinal Microecology in Wenchang Chicken under Hot-Humid Conditions[J]. Chinese Journal of Animal Nutrition, 2022 , 34(8) : 5048 -5062 . DOI: 10.3969/j.issn.1006-267x.2022.08.029

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