Effects of Dietary Supplementation with Clostridium butyricum on Growth Performance and Rectal Microflora of Fattening Goats

ZHANG Chengrui; YU Qingyuan; WANG Jihong; SUN Yukun; WAN Tao; YU Yidong; ZHANG Yonggen

doi:10.3969/j.issn.1006-267x.2022.08.038

2022 , Vol. 34 >Issue 8: 5146 - 5155

DOI: https://doi.org/10.3969/j.issn.1006-267x.2022.08.038

RESEARCH PAPER

Effects of Dietary Supplementation with Clostridium butyricum on Growth Performance and Rectal Microflora of Fattening Goats

ZHANG Chengrui ,
YU Qingyuan ,
WANG Jihong ,
SUN Yukun ,
WAN Tao ,
YU Yidong ,
ZHANG Yonggen

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1. College of Animal Science and Technology, Northeast Agricultural University, Harbin 150030, China;
2. College of Grassland, Resources and Environment, Inner Mongolia Agricultural University, Hohhot 010010, China;
3. Ordos Academy of Agriculture and Animal Husbandry, Ordos 017000, China

Received date: 2022-01-20

Online published: 2022-08-11

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Abstract

The purpose of this experiment was to investigate the effects of dietary Clostridium butyricum (C. butyricum) on growth performance and rectal microflora of fattening goats. A total of 16 healthy 6-month-old male Albas goats with similar body weight of (22.00±2.09) kg were selected and randomly divided into 2 groups with 8 replicates per group, and 1 goat per replicate: the control group was fed a basal diet (concentrate to forage ratio = 65：35); the C. butyricum group was fed the basal diet+2.0×10⁸ CFU/kg C. butyricum (≥1.0×10⁹ CFU/g). The pre-feeding period was 14 days, and the experimental period was 56 days. The results showed that compared with the control group, the supplementation of C. butyricum to the diet had no significant effects on the growth performance, nutrient apparent digestibility and nitrogen metabolism of fattening goats (P>0.05), but adding C. butyricum could significantly increase plasma glucose (GLU) and total cholesterol (TC), high-density lipoprotein (HDL) and low-density lipoprotein (LDL) contents (P<0.05). The activity of aspartate aminotransferase (AST) and the contents of total protein (TP) and globulin (GLB) in plasma showed an increasing trend (0.05<P<0.10). Compared with the control group, the relative abundance of Christensenaceae _R-7_group in the rectum of fattening goats fed with C. butyricum was significantly increased (P<0.05), while the relative abundance of Ruminobacteriaceae _UCG-014 was significantly decreased (P<0.05). The relative abundance of Clostridium in the rectum showed a decreasing trend (0.05＜P＜0.10), while the relative abundance of Campylobacter showed an increasing trend (0.05＜P＜0.10). In conclusion, high-concentrate diets supplementation with C. butyricum can increase the contents of GLU and HDL in goat plasma, and improve the rectal microflora structure by up-regulating the relative abundance of beneficial bacteria, but there is no significant effect on the growth performance of fattening goats.

Key words： Clostridium butyricum; fattening goats; growth performance; plasma biochemical indexes; rectal microflora

Cite this article

ZHANG Chengrui , YU Qingyuan , WANG Jihong , SUN Yukun , WAN Tao , YU Yidong , ZHANG Yonggen . Effects of Dietary Supplementation with Clostridium butyricum on Growth Performance and Rectal Microflora of Fattening Goats[J]. Chinese Journal of Animal Nutrition, 2022 , 34(8) : 5146 -5155 . DOI: 10.3969/j.issn.1006-267x.2022.08.038

References

[1] ZHANG J,CHEN X Y,LIU P,et al.Dietary Clostridium butyricum induces a phased shift in fecal microbiota structure and increases the acetic acid-producing bacteria in a weaned piglet model[J].Journal of Agricultural and Food Chemistry,2018,66(20):5157-5166.
[2] HAN Y S,TANG C H,LI Y,et al.Effects of dietary supplementation with Clostridium butyricum on growth performance,serum immunity,intestinal morphology,and microbiota as an antibiotic alternative in weaned piglets[J].Animals,2020,10(12):2287.
[3] CAI L Y,YU J K,HARTANTO R,et al.Dietary supplementation with Saccharomyces cerevisiae,Clostridium butyricum and their combination ameliorate rumen fermentation and growth performance of heat-stressed goats[J].Animals,2021,11(7):2116.
[4] 王腾浩.新型丁酸梭菌筛选及其对断奶仔猪生长性能和肠道功能影响的研究[D].博士学位论文.杭州:浙江大学,2015.WANG T H.Screening of a novel Clostridium butyricum and its administration on growth performance and gut function in weaning piglets[D].Ph.D.Thesis.Hangzhou:Zhejiang University,2015.(in Chinese)
[5] ZHANG C R,YU Q Y,WANG J H,et al.Effects of dietary supplementation with Clostridium butyricum on growth performance,apparent digestibility,blood metabolites,ruminal fermentation and bacterial communities of fattening goats[J].Frontiers in Nutrition,2022,9:888191.
[6] SEKI H,SHIOHARA M,MATSUMURA T,et al.Prevention of antibiotic-associated diarrhea in children by Clostridium butyricum MIYAIRI[J].Pediatrics International,2003,45(1):86-90.
[7] HAGIHARA M,KUROKI Y,ARIYOSHI T,et al.Clostridium butyricum modulates the microbiome to protect intestinal barrier function in mice with antibiotic-induced dysbiosis[J].iScience,2020,23(1):100772.
[8] XU L P,SUN X L,WAN X H,et al.Dietary supplementation with Clostridium butyricum improves growth performance of broilers by regulating intestinal microbiota and mucosal epithelial cells[J].Animal Nutrition,2021,7(4):1105-1114.
[9] 李文茜.丁酸梭菌对犊牛及育成牛生长性能、血液指标和瘤胃发酵的影响[D].硕士学位论文.哈尔滨:东北农业大学,2019.LI W X.Effects of Clostridium butyricum on growth performance, blood index and rumen fermentation in calves and bred cattle[D].Master's Thesis.Harbin:Northeast Agricultural University,2019.(in Chinese)
[10] 陈浅,董瑷榕,陈菊红,等.不同精粗比日粮中添加丁酸梭菌对断奶羔羊生长性能、血清指标及经济效益的影响[J].饲料工业,2020,41(23):6-14.CHEN J,DONG A R,CHEN J H,et al.Effects of adding Clostridium butyricum to diets with different concentrate to forage ratios on growth performance,serum indexes and economic benefits of weaned lambs[J].Feed Industry,2020,41(23):6-14.(in Chinese)
[11] AOAC.Official methods of analysis[M].14th ed.Arlington,VA:Association of Official Analytical Chemists,1984:152-169.
[12] VAN SOEST P J,ROBERTSON J B,LEWIS B A.Methods for dietary fiber,neutral detergent fiber,and nonstarch polysaccharides in relation to animal nutrition[J].Journal of Dairy Science,1991,74(10):3583-3597.
[13] VALADARES R F,BRODERICK G A,VALADARES FILHO S C,et al.Effect of replacing alfalfa silage with high moisture corn on ruminal protein synthesis estimated from excretion of total purine derivatives[J].Journal of Dairy Science,1999,82(12):2686-2696.
[14] LEONARDI C,STEVENSON M,ARMENTANO L E.Effect of two levels of crude protein and methionine supplementation on performance of dairy cows[J].Journal of Dairy Science,2003,86(12):4033-4042.
[15] WANG W W,WANG J,ZHANG H J,et al.Effects of Clostridium butyricum on production performance and intestinal absorption function of laying hens in the late phase of production[J].Animal Feed Science and Technology,2020,264:114476.
[16] LIANG J,RAZA S H A,KOU S S,et al.Effect of Clostridium butyricum on plasma immune function,antioxidant activity and metabolomics of weaned piglets[J].Livestock Science,2020,241:104267.
[17] 何家俊,吴汉葵,杨昕涧,等.丁酸梭菌对犊牛生长性能和血清生化、抗氧化、免疫指标及粪便微生物数量的影响[J].动物营养学报,2021,33(9):5076-5085.HE J J,WU H K,YANG X J,et al.Effects of Clostridium butyricum on growth performance,serum biochemical,antioxidant,immune indexes and fecal microorganism number of calves[J].Chinese Journal of Animal Nutrition,2021,33(9):5076-5085.(in Chinese)
[18] MU C T,YANG W J,WANG P J,et al.Effects of high-concentrate diet supplemented with grape seed proanthocyanidins on growth performance,liver function,meat quality,and antioxidant activity in finishing lambs[J].Animal Feed Science and Technology,2020,266:114518.
[19] SCHÖNHERR-HELLEC S,KLEIN G,DELANNOY J,et al.Comparative phenotypic analysis of "Clostridium neonatale" and Clostridium butyricum isolates from neonates[J].Anaerobe,2017,48:76-82.
[20] SAULS-HIESTERMAN J A,BANUELOS S,ATANASOV B,et al.Physiologic responses to feeding rumen-protected glucose to lactating dairy cows[J].Animal Reproduction Science,2020,216:106346.
[21] WANG Y P,CAI M,HUA D K,et al.Metabolomics reveals effects of rumen-protected glucose on metabolism of dairy cows in early lactation[J].Animal Feed Science and Technology,2020,269:114620.
[22] VAN DORLAND H A,RICHTER S,MOREL I,et al.Variation in hepatic regulation of metabolism during the dry period and in early lactation in dairy cows[J].Journal of Dairy Science,2009,92(5):1924-1940.
[23] ZAMUNER F,CAMERON A W N,CARPENTER E K,et al.Endocrine and metabolic responses to glucose,insulin,and adrenocorticotropin infusions in early-lactation dairy goats of high and low milk yield[J].Journal of Dairy Science,2020,103(12):12045-12058.
[24] MCLAUGHLIN C L,BYATT J C,HEDRICK H B,et al.Performance,clinical chemistry,and carcass responses of finishing lambs to recombinant bovine somatotropin and bovine placental lactogen[J].Journal of Animal Science,1993,71(12):3307-3318.
[25] DING H,LIU W J,ERDENE K,et al.Effects of dietary supplementation with Allium mongolicum Regel extracts on growth performance,serum metabolites,immune responses,antioxidant status,and meat quality of lambs[J].Animal Nutrition,2021,7(2):530-538.
[26] PLAIZIER J C,KHAFIPOUR E,LI S,et al.Subacute ruminal acidosis (SARA),endotoxins and health consequences[J].Animal Feed Science and Technology,2012,172(1/2):9-21.
[27] GUO Y Q,XU X F,ZOU Y,et al.Changes in feed intake,nutrient digestion,plasma metabolites,and oxidative stress parameters in dairy cows with subacute ruminal acidosis and its regulation with pelleted beet pulp[J].Journal of Animal Science and Biotechnology,2013,4(1):31.
[28] MOHAN B,KADIRVEL R,BHASKARAN M,et al.Effect of probiotic supplementation on serum/yolk cholesterol and on egg shell thickness in layers[J].British Poultry Science,1995,36(5):799-803.
[29] JIANG X,XU H J,CUI Z Q,et al.Effects of supplementation with Lactobacillus plantarum 299v on the performance,blood metabolites,rumen fermentation and bacterial communities of preweaning calves[J].Livestock Science,2020,239:104120.
[30] SANDERS M E.Considerations for use of probiotic bacteria to modulate human health[J].The Journal of Nutrition,2000,130(2S Suppl.):384S-390S.
[31] 王丽华,徐宏建,张全宇,等.不同剂量25羟基维生素D₃对断奶前犊牛生长性能、营养物质表观消化率、血浆代谢物及粪便菌群的影响[J].动物营养学报,2022,34(1):404-412.WANG L H,XU H J,ZHANG Q Y,et al.Effects of different doses of 25-hydroxyvitamin D₃ on growth performance,nutrient apparent digestibility,plasma metabolites and fecal flora of pre-weaning calves[J].Chinese Journal of Animal Nutrition,2022,34(1):404-412.(in Chinese)
[32] CHENG C,WEI H,PENG J.PSVII-40 maternal soluble fiber diet during pregnancy influences growth performance,intestinal microbiota and intestinal permeability in piglets[J].Journal of Animal Science,2018,96(Suppl.3):329-330.
[33] ZHOU Y,ZHANG M H,LIU Q X,et al.The alterations of tracheal microbiota and inflammation caused by different levels of ammonia exposure in broiler chickens[J].Poultry Science,2021,100(2):685-696.
[34] LI J W,FANG B,PANG G F,et al.Age-and diet-specific effects of chronic exposure to chlorpyrifos on hormones,inflammation and gut microbiota in rats[J].Pesticide Biochemistry and Physiology,2019,159:68-79.
[35] NAUTA M,BOLTON D,CROTTA M,et al.An updated assessment of the effect of control options to reduce Campylobacter concentrations in broiler caeca on human health risk in the European Union[J/OL].Microbial Risk Analysis,2021,100197[2021-12-05].https://doi.org/10.1016/j.mran.2021.100197.
[36] WVRFEL S D F R,PRATES D D F,KLEINUBING N R,et al.Comprehensive characterization reveals antimicrobial-resistant and potentially virulent Campylobacter isolates from poultry meat products in Southern Brazil[J].LWT,2021,149:111831.

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