Effects of Dietary Eucalyptus Oil and Anise Oil on Methane Production and Rumen Methanogens Community of Mutton Sheep

TANG Weixuan; ZHAO Chen; JIA Miao; LI Yan; LIU Shutian; LI Yanling

doi:10.3969/j.issn.1006-267x.2022.08.040

Chinese Journal of Animal Nutrition >

2022 , Vol. 34 >Issue 8: 5168 - 5179

DOI: https://doi.org/10.3969/j.issn.1006-267x.2022.08.040

RESEARCH PAPER

Effects of Dietary Eucalyptus Oil and Anise Oil on Methane Production and Rumen Methanogens Community of Mutton Sheep

TANG Weixuan ,
ZHAO Chen ,
JIA Miao ,
LI Yan ,
LIU Shutian ,
LI Yanling

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College of Animal Science and Technology, Beijing University of Agriculture, Beijing 102206, China

Received date: 2022-01-10

Online published: 2022-08-11

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Abstract

This study was conducted to evaluate the effects of dietary plant essential oils (EOs) on methane (CH₄) production and rumen methanogens community of mutton sheep. The experiment used a 3×3 Latin square design, six healthy F1 generation hybrid fistulas Duhan mutton sheep with the body weight of (64.45±8.56) kg were randomly allocated to 3 groups with 2 sheep per group, and the 3 groups were control group (without EOs), eucalyptus oil group [supplemented with 500 mg/(head·d) eucalyptus oil] and anise oil group [supplemented with 500 mg/(head·d) anise oil]. A total of 3 stages of experiment were conducted for 22 days each, including 14 days of pre-feeding and 8 days of formal experiment. During the experiment, the CH₄ production of mutton sheep was measured by respiratory calorimetry chamber. At the end of the experiment, rumen digesta was collected and the microbial number of mutton sheep rumen was determined by fluorescence quantitative PCR, Illumina MiSeq high-throughput sequencing technique was used to determine the changes of the rumen methanogens community. The results showed that, compared with the control group, diet supplemented with eucalyptus oil had no significant effects on the CH₄ production of mutton sheep (P>0.05), but the CH₄ production had a decreasing tendency when diet supplemented with anise oil (P=0.08). The supplementation of eucalyptus oil or anise oil had no significant effect on the number of total bacteria, total fungi, total protozoa and total methanogens in rumen of mutton sheep (P>0.05). Compared with the control group, diet supplemented with eucalyptus oil significantly decreased the relative abundances of Methanobrevibacter ruminantium and unclassified Methanobrevibacter (P<0.05), and significantly increased the relative abundance of Methanobrevibacter euryarchaeote (P<0.05); diet supplemented with anise oil significantly decreased the relative abundance of Methanobrevibacter ruminantium (P<0.05), and significantly increased the relative abundances of the unclassified Methanobrevibacter and Methanobrevibacter euryarchaeote (P<0.05). In conclusion, the supplementation of 500 mg/(head·d) eucalyptus oil or anise oil in diets of mutton sheep has no significant effect on the rumen microbial number, but affects the rumen methanogens community. The numerical decrease of anise oil on CH₄ production may be due to the decrease in the number of Methanobrevibacter ruminantium.

Key words： eucalyptus oil; anise oil; CH₄ production; methanogens community; mutton sheep

Cite this article

TANG Weixuan , ZHAO Chen , JIA Miao , LI Yan , LIU Shutian , LI Yanling . Effects of Dietary Eucalyptus Oil and Anise Oil on Methane Production and Rumen Methanogens Community of Mutton Sheep[J]. Chinese Journal of Animal Nutrition, 2022 , 34(8) : 5168 -5179 . DOI: 10.3969/j.issn.1006-267x.2022.08.040

References

[1] COBELLIS G,TRABALZA-MARINUCCI M,YU Z T.Critical evaluation of essential oils as rumen modifiers in ruminant nutrition:a review[J].Science of the Total Environment,2016,545/546:556-568.
[2] JOUANY J P,MORGAVI D P.Use of'natural'products as alternatives to antibiotic feed additives in ruminant production[J].Animal,2007,1(10):1443-1466.
[3] JAHANI-AZIZABADI H,DURMIC Z,VADHANABHUTI J.Effect of some Australian native shrubs essential oils on in vitro rumen microbial fermentation of a high-concentrate diet[J].Journal of Animal and Plant Sciences,2019,29(1):8-15.
[4] GARCIA F,VERCOE P E,MARTÍNEZ M J,et al.Essential oils from Lippia turbinata and Tagetes minuta persistently reduce in vitro ruminal methane production in a continuous-culture system[J].Animal Production Science,2018,59(4):709-720.
[5] GARCIA F,COLOMBATTO D,BRUNETTI M A,et al.The reduction of methane production in the in vitro ruminal fermentation of different substrates is linked with the chemical composition of the essential oil[J].Animals,2020,10(5):786.
[6] ZHOU R,WU J P,LANG X,et al.Effects of oregano essential oil on in vitro ruminal fermentation,methane production,and ruminal microbial community[J].Journal of Dairy Science,2020,103(3):2303-2314.
[7] LEI Z M,ZHANG K,LI C,et al.Ruminal metagenomic analyses of goat data reveals potential functional microbiota by supplementation with essential oil-cobalt complexes[J].BMC Microbiology,2019,19(1):30.
[8] LEE S S,KIM D H,PARADHIPTA D H V,et al.Effects of wormwood (Artemisia montana) essential oils on digestibility,fermentation indices,and microbial diversity in the rumen[J].Microorganisms,2020,8(10):1605.
[9] ALEKSIC SABO V,KNEZEVIC P.Antimicrobial activity of Eucalyptus camaldulensis Dehn.plant extracts and essential oils:a review[J].Industrial Crops and Products,2019,132:413-429.
[10] BAYALA B,BASSOLE I H N,GNOULA C,et al.Chemical composition,antioxidant,anti-inflammatory and anti-proliferative activities of essential oils of plants from Burkina Faso[J].Plos One,2014,9(3):e92122.
[11] AKDEMIR EVRENDILEK G.Empirical prediction and validation of antibacterial inhibitory effects of various plant essential oils on common pathogenic bacteria[J].International Journal of Food Microbiology,2015,202:35-41.
[12] KORINEK M,HANDOUSSA H,TSAI Y H,et al.Anti-inflammatory and antimicrobial volatile oils:fennel and cumin inhibit neutrophilic inflammation via regulating calcium and MAPKs[J].Frontiers in Pharmacology,2021,12:674095.
[13] KSOUDA G,SELLIMI S,MERLIER F,et al.Composition,antibacterial and antioxidant activities of Pimpinella saxifraga essential oil and application to cheese preservation as coating additive[J].Food Chemistry,2019,288:47-56.
[14] SALLAM S M A,BUENO I C S,BRIGIDE P,et al.Efficacy of eucalyptus oil on in vitro ruminal fermentation and methane production[M]//PAPACHRISTOU T G,PARISSI Z M,BEN SALEM H,et al.Nutritional and foraging ecology of sheep and goats.Zaragoza:CIHEAM/FAO/NAGREF,2009:267-272.
[15] PATRA A K,YU Z T.Effects of essential oils on methane production and fermentation by,and abundance and diversity of,rumen microbial populations[J].Applied and Environmental Microbiology,2012,78(12):4271-4280.
[16] CHAHAARDOLI A,SOROOR M N,FOROUGHI A.The effects of anise (Pimpinella anisum) essential oil and extract on in vitro rumen fermentation parameters and protozoa population of sheep[J].International Journal of Veterinary Science,2018,7(1):21-27.
[17] 赵丽萍.硝酸盐对肉牛瘤胃发酵、微生物多样性、血液生化及抗氧化性能的影响[D].博士学位论文.北京:中国农业大学,2015.ZHAO L P.Effects of nitrate on rumen fermentation,microbe diversity,blood biochemical and antioxidative ability[D].Ph.D.Thesis.Beijing:China Agricultural University,2015.(in Chinese)
[18] TYMENSEN L D,MCALLISTER T A.Community structure analysis of methanogens associated with rumen protozoa reveals bias in universal archaeal primers[J].Applied and Environmental Microbiology,2012,78(11):4051-4056.
[19] LUO Y H,WRIGHT A D,LI Y L,et al.Diversity of methanogens in the hindgut of captive white rhinoceroses,Ceratotherium simum[J].BMC Microbiology,2013,13:207.
[20] AMATO K R,YEOMAN C J,KENT A,et al.Habitat degradation impacts black howler monkey (Alouatta pigra) gastrointestinal microbiomes[J].The ISME Journal,2013,7(7):1344-1353.
[21] LAN W,YANG C L.Ruminal methane production:associated microorganisms and the potential of applying hydrogen-utilizing bacteria for mitigation[J].Science of the Total Environment,2019,654:1270-1283.
[22] HASSAN F U,ARSHAD M A,EBEID H M,et al.Phytogenic additives can modulate rumen microbiome to mediate fermentation kinetics and methanogenesis through exploiting diet-microbe interaction[J].Frontiers in Veterinary Science,2020,7:575801.
[23] 李斌昌,董利锋,成述儒,等.不同精粗比日粮对反刍动物甲烷排放的调控[J].家畜生态学报,2019,40(1):1-6.LI B C,DONG L F,CHENG S R,et al.Regulation of methane emissions from ruminants with different crude-to-ratio diets[J].Journal of Domestic Animal Ecology,2019,40(1):1-6.(in Chinese)
[24] CHAVES A V,HE M L,YANG W Z,et al.Effects of essential oils on proteolytic,deaminative and methanogenic activities of mixed ruminal bacteria[J].Canadian Journal of Animal Science,2008,88(1):117-122.
[25] YE D,KARNATI S K R,WAGNER B,et al.Essential oil and monensin affect ruminal fermentation and the protozoal population in continuous culture[J].Journal of Dairy Science,2018,101(6):5069-5081.
[26] EL-ZAIAT H M,ABDALLA A L.Potentials of patchouli (Pogostemon cablin) essential oil on ruminal methanogenesis,feed degradability,and enzyme activities in vitro[J].Environmental Science and Pollution Research,2019,26(29):30220-30228.
[27] CASTAÑEDA-CORREA A,CORRAL-LUNA A,HUME M E,et al.Effects of thymol and carvacrol,alone or in combination,on fermentation and microbial diversity during in vitro culture of bovine rumen microbes[J].Journal of Environmental Science and Health-Part B:Pesticides,Food Contaminants,and Agricultural Wastes,2019,54(3):170-175.
[28] KOUAZOUNDE J B,JIN L,ASSOGBA F M,et al.Effects of essential oils from medicinal plants acclimated to benin on in vitro ruminal fermentation of Andropogon gayanus grass[J].Journal of the Science of Food and Agriculture,2015,95(5):1031-1038.
[29] COBELLIS G,TRABALZA-MARINUCCI M,MARCOTULLIO M C,et al.Evaluation of different essential oils in modulating methane and ammonia production,rumen fermentation,and rumen bacteria in vitro[J].Animal Feed Science and Technology,2016,215:25-36.
[30] DURMIC Z,MOATE P J,ECKARD R,et al.In vitro screening of selected feed additives,plant essential oils and plant extracts for rumen methane mitigation[J].Journal of the Science of Food and Agriculture,2014,94(6):1191-1196.
[31] PATRA A,PARK T,KIM M,et al.Rumen methanogens and mitigation of methane emission by anti-methanogenic compounds and substances[J].Journal of Animal Science and Biotechnology,2017,8:13.
[32] 陆燕.植物精油对瘤胃甲烷产量生成和微生态的影响[D].硕士学位论文.南京:南京农业大学,2009.LU Y.Effects of plant essential oils on ruminal methanogenesis and microbial ecology[D].Master's Thesis.Nanjing:Nanjing Agricultural University,2009.(in Chinese)
[33] 石宁,贾淼,李艳玲.体外产气法研究植物精油对肉羊体外瘤胃发酵参数及甲烷产量的影响[J].动物营养学报,2019,31(1):274-284.SHI N,JIA M,LI Y L.Effects of plant essential oil on rumen fermentation parameters and methane production of mutton sheep in vitro[J].Chinese Journal of Animal Nutrition,2019,31(1):274-284.(in Chinese)
[34] PARASKEVAKIS N.Effects of dietary Greek oregano (Origanum vulgare ssp.hirtum) supplementation on rumen fermentation,enzyme profile and microbial communities in goats[J].Journal of Animal Physiology and Animal Nutrition,2018,102(3):701-705.
[35] VARGAS J E,ANDRÉS S,LÓPEZ-FERRERAS L,et al.Dietary supplemental plant oils reduce methanogenesis from anaerobic microbial fermentation in the rumen[J].Scientific Reports,2020,10(1):1613.
[36] ST-PIERRE B,WRIGHT A D G.Molecular analysis of methanogenic archaea in the forestomach of the alpaca (Vicugna pacos)[J].BMC Microbiology,2012,12:1.
[37] HENDERSON G,COX F,GANESH S,et al.Rumen microbial community composition varies with diet and host,but a core microbiome is found across a wide geographical range[J].Scientific Reports,2015,5:14567.
[38] KALA A,KAMRA D N,AGARWAL N,et al.Insights into metatranscriptome,and cazymes of buffalo rumen supplemented with blend of essential oils[J].Indian Journal of Microbiology,2020,60(4):485-493.
[39] OHENE-ADJEI S,CHAVES A V,MCALLISTER T A,et al.Evidence of increased diversity of methanogenic archaea with plant extract supplementation[J].Microbial Ecology,2008,56(2):234-242.

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