Chinese Journal of Animal Nutrition >

2022 , Vol. 34 >Issue 8: 5190 - 5205

DOI: https://doi.org/10.3969/j.issn.1006-267x.2022.08.042

RESEARCH PAPER

Effects of Adding Enterococcus faecalis, Bacillus subtilis and Their Compound Bacteria on Growth Performance, Nutrient Apparent Digestibility, Rumen Fermentation Indexes and Rumen Microflora of Sheep

  • BAI Tiantian ,
  • CUI Haoran ,
  • ZHAO Linbo ,
  • WANG Yan ,
  • GUO Xuefeng ,
  • ZHOU Xiaoling
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  • 1. College of Animal Science and Technology, Tarim University, Alar 843300, China;
    2. Key Laboratory of Tarim Animal Husbandry Science and Technology, Alar 843300, China

Received date: 2022-01-13

  Online published: 2022-08-11

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Abstract

The purpose of this study was to investigate effects of adding Enterococcus faecalis, Bacillus subtilis and their compound bacteria on growth performance, nutrient apparent digestibility, rumen fermentation indexes and rumen microflora of sheep. Twenty-four adult sheep weighing (32.7±4.7) kg with fistula were selected in the experiment. They were randomly divided into 4 groups: CK group (adding 10 mL fermentation broth), S group (adding 10 mL 1×1010 CFU/mL Enterococcus faecium fermentation broth), K group (adding 10 mL 1×1010 CFU/mL Bacillus subtilis fermentation broth) and F group (adding 5 mL 1×1010 CFU/mL Enterococcus faecium fermentation broth+5 mL 1×1010 CFU/mL Bacillus subtilis fermentation broth), each group included 6 replicates, and each replicate had 1 sheep. The pre-test period was 15 days and the test period was 60 days. The test period was divided into days 1 to 30 (period Ⅰ) and days 31 to 60 (period Ⅱ). Rumen fermentation test was carried out on the days 1 to 3 and days 31 to 33, nutrient apparent digestibility was measured on days 5 to 12 and days 35 to 42, and rumen microflora was measured on day 30 and day 60. The results showed as follows: 1) average daily gain (ADG) of S group and K group was higher than that of CK group in period Ⅰ (P>0.05), feed/gain (F/G) of F group was the highest, acid detergent fiber (ADF) apparent digestibility of S group was significantly higher than that of CK group in period Ⅱ (P＜0.05), and F/G of S group was the highest. 2) At period Ⅰ, there was no significant difference in pH, ammoniacal nitrogen (NH3-N) and volatile fatty acids (VFA) contents between each test groups and CK group (P>0.05). At period Ⅱ, at 6 h, the acetic acid content of K group was significantly lower than that of S group and F group (P<0.05), but had no significant difference compared with CK group (P＞0.05), the propionic acid content of K group was significantly lower than that of CK group and S group (P<0.05), the total volatile fatty acids (TVFA) contents of K group was significantly lower than that of other groups (P<0.05), and at 0 h, the ratio of ethylene to propylene of F group was significantly higher than that of other groups (P<0.05). 3) At period Ⅱ, the relative abundance of uncultured_bacterium_f_F082 of group S was significantly lower than that in group CK (P<0.05), and the relative abundance of Verrucomicrobia in CK group was significantly higher than that in K group and F groups (P<0.05). In summary, the addition of Enterococcus faecium for 60 d can improve the apparent digestibility of nutrients in sheep and regulate the relative abundance of ruminal microflora or genera in sheep. The effect is better than that of Bacillus subtilis, which can be used as a microecological feed additive for green breeding of ruminants.

Key words： Enterococcus faecium; Bacillus subtilis; digestibility; rumen fermentation; rumen microflora

Cite this article

BAI Tiantian , CUI Haoran , ZHAO Linbo , WANG Yan , GUO Xuefeng , ZHOU Xiaoling . Effects of Adding Enterococcus faecalis, Bacillus subtilis and Their Compound Bacteria on Growth Performance, Nutrient Apparent Digestibility, Rumen Fermentation Indexes and Rumen Microflora of Sheep[J]. Chinese Journal of Animal Nutrition, 2022 , 34(8) : 5190 -5205 . DOI: 10.3969/j.issn.1006-267x.2022.08.042

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