Chinese Journal of Animal Nutrition >

2022 , Vol. 34 >Issue 8: 5313 - 5327

DOI: https://doi.org/10.3969/j.issn.1006-267x.2022.08.052

RESEARCH PAPER

Effects of Dietary Sodium Quercetin-5'-Sulfonates on Growth Performance, Antioxidant Capacity and Intestinal Health of Hybrid Groupers (Epinephelus fuscoguttatus ♀×Epinephelus polyphekadion ♂)

  • LUO Jun ,
  • YANG Erjun ,
  • FU Weijie ,
  • HUANG Jiansheng ,
  • XIE Ruitao ,
  • CHEN Gang
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  • 1. Fishery College, Guangdong Ocean University, Zhanjiang 524088, China;
    2. Southern Marine Science and Engineering Guangdong Laboratory (Zhanjiang), Zhanjiang 524025, China;
    3. Guangdong Evergreen Feed Industry Co., Ltd., Zhanjiang 524022, China

Received date: 2021-11-30

  Online published: 2022-08-11

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Abstract

This experiment studied the effects of adding sodium quercetin-5'-sulfonate (QS) in the diet on growth performance, antioxidant capacity, intestinal nuclear factor-κB (NF-κB) signaling pathway-related gene expression and intestinal microflora of hybrid groupers (Epinephelus fuscoguttatus ♀×Epinephelus polyphekadion ♂). The QS was synthesized and characterized firstly in this experiment and then added it to a basal diet at the additions of 0 (FM group), 0.80 (QSL group), 1.60 (QSM group), 3.20 mmol/kg (QSH group), and to fed hybrid grouper juveniles [body weight was (10.10±0.02) g] for 56 days. Each test diet was fed to 3 glass buckets and each glass bucket was stocked with 25 fish. The results showed as follows: 1) adding QS had no significant effects on the weight gain rate (WGR), specific growth rate (SGR), survival rate (SR) and feed conversion ratio (FCR) of hybrid groupers compared with the FM group (P>0.05). 2) Compared with the FM group, the addition of 0.80 and 1.60 mmol/kg QS significantly increased the liver superoxide dismutase (SOD), glutathione peroxidase (GPx) and catalase (CAT) activities (P<0.05), and the addition of 3.20 mmol/kg QS significantly increased the liver CAT activity of hybrid groupers (P<0.05). 3) The QS-added groups of hybrid groupers had significantly lower intestinal GPx activity (P<0.05) and significantly increased malondialdehyde (MDA) content (P<0.05) compared with the FM group. 4) Compared with the FM group, the relative expression levels of intestinal tumor necrosis factor-α (TNF-α), TNF receptor-associated factor-2 (TRAF-2), TNF receptor superfamily member 1A (TNFRSF1A), interferon-γ (IFN-γ), mitogen-activated protein kinase kinase kinase 7 (MAP3K7), MAP3K7-binding protein 3 (TAB3) in the QSL group were significantly up-regulated (P<0.05); the relative expression levels of intestinal TNF-α and IFN-γ were significantly up-regulated (P<0.05), and the relative expression levels of nuclear factor-κBp65 subunit (NF-κBp65) and NF-κB inhibitor-α (IκB-α), TAB3 and MAP3K7 were significantly down-regulated in the QSM and QSH groups (P<0.05). 5) The α diversity of the intestinal microflora of the three QS-added groups was not significantly different from that of the FM group (P>0.05); the β diversity showed an obvious clustering trend among the four groups, with the QSL and QSM groups moving away from the FM group. 6) Compared with the FM group, the relative abundances of Bacteroidetes and Akkermansia in the intestinal microflora of the QSL group were significantly decreased (P<0.05), and the relative abundances of Actinobacteria and Bifidobacterium were significantly increased (P<0.05); the relative abundances of Firmicutes and Ruminococcaceae_UCG-005 in the intestinal microflora of the QSM group were significantly decreased (P<0.05). In summary, adding QS to the diet do not promote the growth of hybrid groupers, but can improve its hepatic antioxidant capacity; QS induces an intestinal inflammatory response in the hybrid groupers by increasing intestinal oxidative stress, changing the structure of intestinal microflora and activating the NF-κB signaling pathway in the intestine.

Key words： quercetin-5'-sodium sulfonate; hybrid groupers (Epinephelus fuscoguttatus ♀×Epinephelus polyphekadion); nuclear factor-κB; intestinal microflora; inflammation

Cite this article

LUO Jun , YANG Erjun , FU Weijie , HUANG Jiansheng , XIE Ruitao , CHEN Gang . Effects of Dietary Sodium Quercetin-5'-Sulfonates on Growth Performance, Antioxidant Capacity and Intestinal Health of Hybrid Groupers (Epinephelus fuscoguttatus ♀×Epinephelus polyphekadion ♂)[J]. Chinese Journal of Animal Nutrition, 2022 , 34(8) : 5313 -5327 . DOI: 10.3969/j.issn.1006-267x.2022.08.052

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