Effects of Different LED Spectral Environments on Muscle Nutritional Composition and Quality of Juvenile Sebastes schlegeli

SUN Fei; WU Yanling; CAI Haowei; LIU Songtao; LI Xin; FANG Yingying; LIU Ying; HAN Rui; MA He

doi:10.3969/j.issn.1006-267x.2022.08.053

2022 , Vol. 34 >Issue 8: 5328 - 5341

DOI: https://doi.org/10.3969/j.issn.1006-267x.2022.08.053

RESEARCH PAPER

Effects of Different LED Spectral Environments on Muscle Nutritional Composition and Quality of Juvenile Sebastes schlegeli

SUN Fei ,
WU Yanling ,
CAI Haowei ,
LIU Songtao ,
LI Xin ,
FANG Yingying ,
LIU Ying ,
HAN Rui ,
MA He

Expand

1. Key Laboratory of Environment Controlled Aquaculture Ministry of Education, Dalian Ocean University, Dalian 116023, China;
2. College of Marine Technology and Environment, Dalian Ocean University, Dalian 116023, China;
3. College of Fisheries and Life Science, Dalian Ocean University, Dalian 116023, China;
4. College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310058, China

Received date: 2022-02-11

Online published: 2022-08-11

Fold

Abstract

In order to explore the effects of different LED spectral environments on the muscle nutritional composition and quality of juvenile Sebastes schlegelii, a total of 750 juveniles [body weight: (38.80±0.43) g; body length: (10.20±0.17) cm] were equally divided in five groups, which were blue light group (BLG), green light group (GLG), yellow light group (YLG), red light group (RLG) and white light group (WLG), and those groups were cultured under different LED spectral environments (blue light, green light, yellow light, red light and white light), The photoperiod was set as 12L ∶ 12D (controlled by electronic timer), and the illuminance was (250±20) mW/m². There were 3 replicates in each group with 50 juveniles in each replicate. The experimental period was 60 days. At the end of the experiment, two fish were randomly selected from each replicate, and muscle samples were sampled to determine muscle fiber morphology, muscle routine nutritional component contents, muscle amino acid and fatty acid composition of juvenile Sebastes schlegeli, and the muscle nutritional quality was analyzed. The results showed as follows: 1) the diameter of muscle fiber in BLG, GLG and RLG was significantly higher than that in YLG and WLG (P<0.05). 2) The content of muscle crude fat in WLG was the highest, which was significantly higher than that in RLG and YLG (P<0.05), but there was no significant difference among WLG, GLG and BLG (P>0.05); in addition, there were no significant differences in the contents of muscle ash, moisture and crude protein among groups (P>0.05). 3) From high to low, the content of muscle collagen was WLG>BLG>GLG>YLG>RLG. 4) Seventeen amino acids (tryptophan was not measured) were detected in the muscle of juvenile Sebastes schlegeli cultured under different spectral environments, including 8 nonessential amino acids (NEAA), 2 half-essential amino acids (HEAA) and 7 essential amino acids (EAA). There were no significant differences in the contents of other 16 amino acids among groups (P＞0.05) except glycine. Among the 17 amino acids, the content of glutamate was the highest in all groups. The content of essential amino acids in the muscle of juvenile Sebastes schlegeli was the highest in YLG, followed by GLG. The amino acid score (AAS) and chemical score (CS) of YLG and GLG were higher. 5) The muscle fatty acid composition of Sebastes schlegeli was similar under different spectral environments, and 16 kinds of fatty acids were detected. The contents of all fatty acids were not significantly different among groups (P>0.05). In conclusion, different LED spectral environments have a certain impact on the muscle nutritional composition and quality of juvenile Sebastes schlegeli. Compared with red light and yellow light, blue light, green light and white light have a slight improvement on the muscle nutritional quality of juvenile Sebastes schlegeli.

Key words： Sebastes schlegeli; LED spectrum; muscle; amino acid; fatty acids; nutritional composition; nutritional quality

Cite this article

SUN Fei , WU Yanling , CAI Haowei , LIU Songtao , LI Xin , FANG Yingying , LIU Ying , HAN Rui , MA He . Effects of Different LED Spectral Environments on Muscle Nutritional Composition and Quality of Juvenile Sebastes schlegeli[J]. Chinese Journal of Animal Nutrition, 2022 , 34(8) : 5328 -5341 . DOI: 10.3969/j.issn.1006-267x.2022.08.053

References

[1] 逯尚尉,刘兆普,余燕.不同饵料对点带石斑鱼幼鱼生长、营养成分及组织消化酶活性的影响[J].上海海洋大学学报,2010,19(5):648-653.LU S W,LIU Z P,YU Y.Effect of different diets on growth,nutritive composition and digestive enzyme activities of juvenile Epinephelus malabaricus[J].Journal of Shanghai University,2010,19(5):648-653.(in Chinese)
[2] KARAKATSOULI N,PAPOUTSOGLOU S E,PIZZONIA G,et al.Effects of light spectrum on growth and physiological status of gilthead seabream Sparus aurata and rainbow trout Oncorhynchus mykiss reared under recirculating system conditions[J].Aquacultural Engineering,2007,36(3):302-309.
[3] SIERRA-FLORES R,DAVIE A,GRANT B,et al.Effects of light spectrum and tank background colour on Atlantic cod (Gadus morhua) and turbot (Scophthalmus maximus) larvae performances[J].Aquaculture,2016,450:6-13.
[4] 魏平平,李鑫,张俊鹏,等.LED光谱对红鳍东方鲀仔稚鱼形态性状及生长相关基因表达的影响[J].渔业科学进展,2020,41(1):162-168.WEI P P,LI X,ZHANG J P,et al.Effects of LED spectra on morphological characters and gene expression of growth in Takifugu rubripes larvae[J].Progress in Fishery Sciences,2020,41(1):162-168.(in Chinese)
[5] 刘松涛,李伊晗,李鑫,等.不同LED光谱对红鳍东方鲀幼鱼生长、摄食及消化酶活性的影响[J].中国水产科学,2021,28(8):1011-1019.LIU S T,LI Y H,LI X,et al.Effects of LED spectra on growth,feeding,and digestive enzyme activities of juvenile Takifugu rubripes[J].Journal of Fishery Sciences of China,2021,28(8):1011-1019.(in Chinese)
[6] MIGAUD H,COWAN M,TAYLOR J,et al.The effect of spectral composition and light intensity on melatonin,stress and retinal damage in post-smolt Atlantic salmon,Salmo salar[J].Aquaculture,2007,270(1/4):390-404.
[7] 李鑫,魏平平,刘松涛,等.不同光周期下欧洲舌齿鲈幼鱼生长、摄食和肌肉营养成分的比较[J].中国水产科学,2020,27(9):1062-1074.LI X,WEI P P,LIU S T,et al.Comparative study of the growth,feeding,amino acid composition,and nutritional quality of Dicentrarchus labrax juveniles under different light photoperiods[J].Journal of Fishery Sciences of China,2020,27(9):1062-1074.(in Chinese)
[8] 狄正凯,李慷,刘如聪,等.光照周期和光照强度对循环水系统中墨瑞鳕的生长、肌肉营养成分及养殖收益的影响[J].水生生物学报,2021,45(4):781-789.DI Z K,LI K,LIU R C,et al.Effects of photoperiod and light intensity on the growth,muscle nutrition and economic performance of Murray cod (Maccullochella peelii) in the recirculating aquaculture system[J].Acta Hydrobiologica Sinica,2021,45(4):781-789.(in Chinese)
[9] VOLPATO G L,BOVI T S,DE FREITAS R H A,et al.Red light stimulates feeding motivation in fish but does not improve growth[J].PLoS One,2013,8(3):e59134.
[10] LOPEZ-BETANCUR D,MORENO I,GUERRERO-MENDEZ C,et al.Effects of colored light on growth and nutritional composition of tilapia,and biofloc as a food source[J].Applied Sciences,2020,10(1):362.
[11] TAKAHASHI A,KASAGI S,MURAKAMI N,et al.Effects of different green light intensities on the growth performance and endocrine properties of barfin flounder Verasper moseri[J].General and Comparative Endocrinology,2018,257:203-210.
[12] 费凡,任纪龙,代明允,等.5种光色环境对欧洲舌齿鲈营养品质的影响[J].动物营养学报,2019,31(5):2431-2441.FEI F,REN J L,DAI M Y,et al.Effects of five kinds of light color environments on nutritional quality of Dicentrarchus labrax[J].Chinese Journal of Animal Nutrition,2019,31(5):2431-2441.(in Chinese)
[13] 高滨,王得宾,牛志兵,等.许氏平鲉鱼苗陆海接力运输技术[J].水产养殖,2021,42(5):48-49.GAO B,WANG D B,NIU Z B,et al.Land sea relay transportation technology of Sebastes schlegeli fry[J].Journal of Aquaculture,2021,42(5):48-49.(in Chinese)
[14] 高滨,王得宾,郑龙华,等.水温变化对近海网箱养殖中许氏平鲉摄食及生长的影响[J].水产养殖,2021,42(5):20-23.GAO B,WANG D B,ZHENG L H,et al.Effects of water temperature change on feeding and growth of Sebastes schleqelii in offshore cage culture[J].Journal of Aquaculture,2021,42(5):20-23.(in Chinese)
[15] 丰爱秀,王芳,孙成峰,等.许氏平鲉人工繁育技术及增殖放流方法[J].科学养鱼,2021(4):64-65.FENG A X,WANG F,SUN C F,et al.Artificial breeding technology and proliferation and release method of Sebastes schlegeli[J].Scientific Fish Farming,2021(4):64-65.(in Chinese)
[16] 房景辉,韦秀梅,王忠全,等.光照强度对许氏平鲉幼鱼的生长、体组分和能量收支的影响[J].中国海洋大学学报(自然科学版),2013,43(9):47-53.FANG J H,WEI X M,WANG Z Q,et al.Effects of light intensity on the growth,biochemical composition and energy budget of juvenile Schlegel's rockfish (Sebastes schlegeli)[J].Periodical of Ocean University of China,2013,43(9):47-53.(in Chinese)
[17] 王梦娅,金超凡,张奉燕,等.许氏平鲉肌肉生长发育的初步研究[J].中国海洋大学学报(自然科学版),2021,51(1):44-50.WANG M Y,JIN C F,ZHANG F Y,et al.Preliminary study on the muscle growth and development of black rockfish (Sebastes schlegelii)[J].Periodical of Ocean University of China,2021,51(1):44-50.(in Chinese)
[18] 吕里康,张思敏,李吉方,等.光周期对许氏平鲉性腺分化过程中形态学、性激素水平及相关基因表达的影响[J].水生生物学报,2020,44(2):319-329.LYU L K,ZHANG S M,LI J F,et al.Effects of photoperiod on morphology,sex hormone levels and gene expression of gonadal differentiation of black rockfish (Sebastes schlegelii)[J].Acta Hydrobiologica Sinica,2020,44(2):319-329.(in Chinese)
[19] 王婧文,王英俊,刘天红,等.6个不同群体许氏平鲉肌肉氨基酸分析及评价[J].中国渔业质量与标准,2020,10(2):65-72.WANG J W,WANG Y J,LIU T H,et al.Analysis and evaluation of muscles amino acids from Sebastes schlegeli in six different regions[J].China Fishery Quality And Standards,2020,10(2):65-72.(in Chinese)
[20] 许晓莹,李小勤,孙文通,等.杜仲对草鱼生长、肌肉品质和胶原蛋白基因表达的影响[J].水产学报,2018,42(5):787-796.XU X Y,LI X Q,SUN W T,et al.Effects of dietary Eucommia ulmoides on growth,flesh quality,and collagen gene expression of grass carp (Ctenopharyngodon idella)[J].Journal of Fisheries of China,2018,42(5):787-796.(in Chinese)
[21] 石军,褚武英,张建社.鱼类肌肉生长分化与基因表达调控[J].水生生物学报,2013,37(6):1145-1152.SHI J,CHU W Y,ZHANG J S,et al.Muscle growth,differentiation and gene expression regulation in fish[J].Acta Hydrobiologica Sinica,2013,37(6):1145-1152.(in Chinese)
[22] PERIAGO M J,AYALA M D,LÓPEZ-ALBORS O,et al.Muscle cellularity and flesh quality of wild and farmed sea bass,Dicentrarchus labrax L.[J].Aquaculture,2005,249(1/4):175-188.
[23] 吴亮,吴洪喜,马建忠,等.光色对豹纹鳃棘鲈幼鱼摄食、生长和存活的影响[J].海洋科学,2016,40(11):44-51.WU L,WU H X,MA J Z,et al.Effect of light color on feeding,growth,and survival of Plectropomus leopardus juveniles[J].Marine Sciences,2016,40(11):44-51.(in Chinese)
[24] 刘群,李吉方,温海深,等.饥饿和再投喂对许氏平鲉幼鱼体组分和糖原含量的影响[J].海洋湖沼通报,2013(1):11-15.LIU Q,LI J F,WEN H S,et al.Study on biochemical compositions after starvation and refeeding in Sebastes schlegeli juvenile[J].Transaction of Oceanology and Limnology,2013(1):11-15.(in Chinese)
[25] 王晓杰,张秀梅,黄国强.低温胁迫对许氏平鲉补偿生长的影响[J].中国水产科学,2006,13(4):566-572.WANG X J,ZHANG X M,HUANG G Q.Compensatory growth of rockfish (Sebastes schlegeli) following low temperature stress[J].Journal of Fishery Sciences of China,2006,13(4):566-572.(in Chinese)
[26] 王珊珊,周德庆,刘楠,等.许氏平鲉鱼皮胶原蛋白与胶原肽的制备及特性研究[J].食品安全质量检测学报,2015,6(10):3976-3983.WANG S S,ZHOU D Q,LIU N,et al.Preparation and characterization of collagen and collagen peptides from the skin of rockfish Sebastes schlegeli[J].Journal of Food Safety&Quality,2015,6(10):3976-3983.(in Chinese)
[27] WANG T,CHENG Y Z,LIU Z P,et al.Effects of light intensity on growth,immune response,plasma cortisol and fatty acid composition of juvenile Epinephelus coioides reared in artificial seawater[J].Aquaculture,2013,414/415:135-139.
[28] 张延华,马国红,宋理平,等.4种鱼肉的基本成分及胶原蛋白含量分析[J].农学学报,2014,4(9):79-81,107.ZHANG Y H,MA G H,SONG L P,et al.Analysis of proximate composition and collagen content of fish muscles[J].Journal of Agriculture,2014,4(9):79-81,107.(in Chinese)
[29] PURSLOW P P.Chapter 3-the structure and growth of muscle[M]//TOLDRAÁ F.Lawrie's meat science.8th ed.Duxford:Woodhead Publishing,2017:49-97.
[30] 汪洋,王稳航.肌内结缔组织的组成、分布及生长调控研究进展[J].中国食品学报,2021,21(7):349-359.WANG Y,WANG W H.Research progress on the composition,distribution and growth regulation of intramuscular connective tissue[J].Journal of Chinese Institute of Food Science and Technology,2021,21(7):349-359.(in Chinese)
[31] 潘孝毅,张琴,李俊,等.饲料中添加甘氨酸可提高大黄鱼(Larimichthys crocea)的抗氧化和抗应激能力[J].渔业科学进展,2017,38(2):91-98.PAN X Y,ZHANG Q,LI J,et al.Dietary glycine improved the capacity of anti-oxidation and Anti-Stress of large yellow croaker (Larimichthys crocea)[J].Progress in Fishery Sciences,2017,38(2):91-98.(in Chinese)
[32] 邴旭文,蔡宝玉,王利平.中华倒刺鲃肌肉营养成分与品质的评价[J].中国水产科学,2005,12(2):211-215.BING X W,CAI B Y,WANG L P,et al.Evaluation of nutritive quality and nutritional components in Spinibarbus sinensis muscle[J] [J].Journal of Fishery Sciences of China,2005,12(2):211-215.(in Chinese)
[33] 罗钦,柯文辉,李冬梅,等.3种特种水产品肌肉中脂肪酸组成比较及主成分综合评价[J].南方农业学报,2019,50(10):2286-2292.LUO Q,KE W H,LI D M,et al.Analysis on fatty acid compositions in muscle of three special aquatic products and comprehensive evaluation of principal components[J].Journal of Southern Agriculture,2019,50(10):2286-2292.(in Chinese)
[34] HENDERSON R J,SARGENT J R,HOPKINS C C E.Changes in the content and fatty acid composition of lipid in an isolated population of the capelin Mallotus villosus during sexual maturation and spawning[J].Marine Biology,1984,78(3):255-263.
[35] 田宇,王忠彦.DHA和EPA的生理功能及研究进展[J].四川食品与发酵,2003(3):44-47.TIAN Y,WANG Z Y.Physiologic functions and research advancement of DHA and EPA[J].Sichuan Food and Fermetation,2003(3):44-47.(in Chinese)
[36] 马爱军,刘新富,翟毓秀,等.野生及人工养殖半滑舌鳎肌肉营养成分分析研究[J].海洋水产研究,2006,27(2):49-54.MA A J,LIU X F,ZHAI Y X,et al.Biochemical composition in muscle of wild and cultivated tongue sole (Cynoglossus semilaevis Günther)[J].Marine Fisheries Research,2006,27(2):49-54.(in Chinese)
[37] MOURENTE G,ODRIOZOLA J M.Effect of broodstock diets on lipid classes and their fatty acid composition in eggs of gilthead sea bream (Sparus aurata L.)[J].Fish Physiology and Biochemistry,1990,8(2):93-101.
[38] 任纪龙,魏平平,费凡,等.LED光谱对舌齿鲈幼鱼摄食、生长和能量分配的影响[J].水产学报,2019,43(8):1821-1829.REN J L,WEI P P,FEI F,et al.Effects of LED spectrum on feeding,growth and energy distribution of juvenile Dicentrarchus labrax[J].Journal of Fisheries of China,2019,43(8):1821-1829.(in Chinese)

Options

Outlines

模态框（Modal）标题

Abstract

Cite this article

References