RESEARCH PAPER

Antagonistic Mechanism of Lycium barbarum Polysaccharides on Immunosuppression of Chicks Based on Metabonomics Technology

  • WANG Jiandong ,
  • TANG Yulin ,
  • WANG Ming ,
  • ZHANG Baosuo ,
  • YANG Fuqiang ,
  • GAO Haihui ,
  • YU Yang ,
  • GUO Yansheng ,
  • LIANG Xiaojun
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  • 1. Institute of Animal Science, Ningxia Academy of Agriculture and Forestry Sciences, Yinchuan 750002, China;
    2. Department of Animal Science and Technology, College of Agriculture, Ningxia University, Yinchuan 750021, China;
    3. Maternal and Child Health Care Hospital of Ningxia, Yinchuan 750001, China;
    4. Ningxia Shunbao Modern Agriculture Co., Ltd., Qingtongxia 751600, China

Received date: 2022-01-13

  Online published: 2022-08-11

Abstract

This experiment was conducted to study the effects of Lycium barbarum polysaccharide on spleen metabolism of immunosuppressive chicks, analyze spleen differential metabolites and screen biomarkers based on metabonomics technology, in order to provide reference basis for Lycium barbarum polysaccharide to antagonize immunosuppression in chicks. One hundred and twenty 7-day-old Hy-line brown chicks were randomly divided into 3 groups, including blank group (NC group), cyclophosphamide group (CY group) and wolfberry polysaccharide group (CYLbGp group), with 40 chicks in each group. Except for the NC group, chicks in the other 2 groups were injected with cyclophosphamide solution (with normal saline as solvent) into the chest muscle at a dose of 80 mg/(kg·d) for 3 consecutive days for modeling, and chicks in the NC group were injected with an equal volume of normal saline into the chest muscle. After modeling, Lycium barbarum polysaccharide with the dose of 5 mg/(kg·d) was added to the drinking water of the CYLbGp group for 30 consecutive days. The spleen was collected from six chicks in each group on day 1 after the end of administration of CYLbGp group. Ultra performance liquid chromatography tandem triple quadrupole time-of-flight mass spectrometry (UHPLC-Triple-TOF-MS) metabolomics technology was used to detect the spleen. Principal component analysis (PCA) method, partial least squares discriminant analysis (PLS-DA) method and orthogonal partial least squares discriminant (OPLS-DA) method were used to analyze the metabolic profiles, and Metaboanalyst 5.0 was used to analyze univariate ROC curves screening for biomarkers. The results showed that the metabolic profile of the spleen in the 3 groups obviously changed, and 14 differential metabolites were confirmed as biomarkers [variable projected importance (VIP)>1, P<0.05], of which 13 metabolites were significantly down-regulated after the intervention of Lycium barbarum polysaccharide (P<0.05), and one metabolite was significantly up-regulated (P<0.05). The above findings suggest that the mechanism of Lycium barbarum polysaccharide antagonizing immunosuppression in chicks may be related to the correction of lipid metabolism disorders, anti-oxidative stress and improvement of spleen function.

Cite this article

WANG Jiandong , TANG Yulin , WANG Ming , ZHANG Baosuo , YANG Fuqiang , GAO Haihui , YU Yang , GUO Yansheng , LIANG Xiaojun . Antagonistic Mechanism of Lycium barbarum Polysaccharides on Immunosuppression of Chicks Based on Metabonomics Technology[J]. Chinese Journal of Animal Nutrition, 2022 , 34(8) : 5354 -5363 . DOI: 10.3969/j.issn.1006-267x.2022.08.055

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