This experiment was conducted to investigate the effects of methionine (Met) on growth and lipid metabolism in HepG2 cells and duck primary hepatocytes. There were two in vitro hepatocyte experiments, including HepG2 cells and duck primary hepatocytes, to study the effects of different Met concentration in medium on the cell viability, lipid deposition and related gene and protein expression of HepG2 cells and duck primary hepatocytes. The results showed as follows: 1) the viability of HepG2 cells when the medium Met concentration 0 μmol/L was significantly lower than other Met concentration (P<0.05), the viability of duck primary hepatocytes when the medium Met concentration 0, 6.25, 12.5 and 25 μmol/L was significantly lower than 100 and 200 μmol/L (P<0.05), with the increased of Met concentration in medium, the plateaus were obtained at 25 and 100 μmol/L Met for HepG2 cells and duck primary hepatocytes, respectively. 2) Compared with 200 μmol/L Met group, the lipid droplet number was increased, the absorbance of oil red O per cell of 0 and 25 μmol/L Met groups was significantly increased (P<0.05), and no difference was observed between 0 and 25 μmol/L Met groups (P>0.05). 3) Compared with 200 μmol/L Met group, the gene expression levels of medium chain acyl-CoA dehydrogenase (ACADM), dihydrolipoyl dehydrogenase (DLD), reduced amide adenine dinucleotide-dehydrogenase ubiquinone Fe-S protein 1 (NDUFS1) in HepG2 cells of 0 and 25 μmol/L Met groups were significantly down regulated (P<0.05), and the NDUFS1 protein expression level of 0 μmol/L Met group was significantly down regulated (P<0.05). 4) Compared with 200 μmol/L Met group, the gene expression levels of malic dehydrogenase (MDH)1, MDH2, DLD, 3-hydroxy-3-methylglutaryl-coA synthase 2 (HMGCS2), electron transfer flavin protein alpha subunit (ETFA), electron transport flavin protein dehydrogenase (ETFED) and NDUFS1 in duck primary hepatocytes of 0, 25 and 100 μmol/L Met groups were significantly down regulated (P<0.05), and the NDUFS1 protein expression level of 0 and 25 μmol/L Met groups was significantly down regulated (P<0.05). It is concluded that Met deficiency can lead to growth inhibition and lipid deposition increasing in HepG2 cells and duck primary hepatocytes, which mainly due to the processes of fatty acid β-oxidation, tricarboxylic acid cycle, respiratory chain electron transport and ketoplasia are depressed, resulting in insufficient energy supply in hepatocytes.
WU Yongbao
,
TANG Jing
,
WEN Zhiguo
,
CAO Junting
,
ZHANG Bo
,
XING Guangnan
,
XIE Ming
,
HU Haifeng
,
CUI Defu
,
HOU Shuisheng
. Effects of Methionine on Growth and Lipid Metabolism in HepG2 Cells and Duck Primary Hepatocytes[J]. Chinese Journal of Animal Nutrition, 2022
, 34(8)
: 5364
-5373
.
DOI: 10.3969/j.issn.1006-267x.2022.08.056
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