RESEARCH PAPER

Alleviating Effects of Fucoidan on Oxidative Stress, Inflammation and Apoptosis in Kidney of Rats

  • KUAI Yunyi ,
  • JIANG Hehao ,
  • HONG Lei ,
  • XU Yuanqing
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  • Collage of Animal Science, Inner Mongolia Agricultural University, Hohhot 010018, China

Received date: 2022-01-26

  Online published: 2022-08-11

Abstract

This study was conducted to investigate the alleviating effects of fucoidan on kidney oxidative damage of rats. A total of 28 SD rats of 8-week-old were randomly divided into 3 groups as control group (n=10), oxidative stress group (n=9) and fucoidan combined with oxidative stress treatment group (n=9). Rats in the control group and the oxidative stress group were fed a basal diet, and those in the fucoidan combined with oxidative stress treatment group were fed the basal diet supplemented with 250 mg/kg fucoidan. The experiment lasted for 20 days. On the 12th day of the experiment, the oxidative stress group and the fucoidan combined with oxidative stress treatment group were injected intraperitoneally with 10 mg/kg BW Diquat to establish the oxidative stress model, and the control group was injected with the same amount of normal saline. After the rats were fed for another 8 days, the blood and kidney tissue samples were collected. The results showed that dietary fucoidan reduced the increase of serum contents of urea nitrogen (P=0.061) and creatinine (P<0.05) as well as the kidney malondialdehyde content (P=0.052) of rats induced by oxidative stress, and increased the activities of catalase (CAT) (P<0.05), superoxide dismutase (SOD) (P=0.068) and glutathione peroxidase (GPx) (P=0.085) in kidney by reversing the downregulation of CAT (P=0.064), superoxide dismutase 2 (SOD2) (P<0.05) and nuclear factor E2 related factor 2 (Nrf2) (P<0.01) gene expression induced by oxidative stress in kidney. In addition, dietary fucoidan down-regulated the increasing gene expression of interleukin-1 (IL-1) (P<0.01), tumor necrosis factor-α (TNF-α) (P=0.079), nuclear factor-κB (NF-κB) (P<0.05), B-cell lymphoma/leukemia-2-associated X protein (Bax) (P<0.01), Caspase-8 (P<0.01), factor associated suicide (Fas) (P<0.01) and factor associated suicide ligand (FasL) (P=0.064) in kidney of rats, and up-regulated the B-cell lymphoma/leukemia-2 (Bcl-2) gene expression and increased the ratio of Bcl-2 to Bax gene expression in kidney (P<0.01). In conclusion, fucoidan can alleviate the oxidative damage of kidney tissues of rats through its antioxidant, anti-inflammatory and anti-apoptotic activities.

Cite this article

KUAI Yunyi , JIANG Hehao , HONG Lei , XU Yuanqing . Alleviating Effects of Fucoidan on Oxidative Stress, Inflammation and Apoptosis in Kidney of Rats[J]. Chinese Journal of Animal Nutrition, 2022 , 34(8) : 5393 -5403 . DOI: 10.3969/j.issn.1006-267x.2022.08.059

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