RESEARCH PAPER

Preparation of Hyperoside-Cyclodextrin Inclusion Complex and Its Biological Antioxidant Activity

  • ZHANG Xinyu ,
  • SU Jianqing ,
  • CHU Xiuling
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  • College of Agronomy and Agricultural Engineering, Liaocheng University, Liaocheng 252000, China

Received date: 2022-03-16

  Online published: 2022-08-11

Abstract

Hyperoside was a kind of flavonoid with low water solubility, in order to improve its water solubility, stability and antioxidant activity, three suitable cyclodextrins (hydroxyethyl-β-cyclodextrin, β-cyclodextrin and methyl-β-cyclodextrin) were selected to coat it. The scanning electron microscope, X-ray diffraction, thin layer chromatography, thermogravimetric analysis and solubility test were used to characterize the three cyclodextrin inclusion complexes of hyperoside prepared by ultrasonic method. On this basis, the antioxidant activity of hyperoside before and after inclusion were compared by in vitro antioxidant and cellular oxidative stress models. The results showed that the inclusion ratio of hyperoside to three kinds of cyclodextrins was mole ratio 1 ∶ 1. The scanning electron microscope, X-ray diffraction and thin layer chromatography showed that hyperoside and cyclodextrin formed inclusion complexes. Among them, the solubility of hyperoside-hydroxyethyl-β-cyclodextrin inclusion complex increased 8.2 times, and it had the highest solubility. The in vitro antioxidant and cellular oxidative stress tests showed that the 50% inhibiting concentration (IC50) of hyperoside on 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-amino-di(2-ethyl-benzothiazoline sulphonic acid-6)ammonium salt (ABTS) free radical scavenging were 7.136 and 8.251 μg/mL, respectively, while the IC50 of hyperoside-hydroxyethyl-β-cyclodextrin inclusion complex were 6.500 and 6.362 μg/mL, respectively. In addition, compared with hyperoside group, the cell malondialdehyde (MDA) content of medium concentration (100 μg/mL) hyperoside-hydroxyethyl-β-cyclodextrin inclusion complex group was extremely significantly decreased (P<0.01), and the cell superoxide dismutase (SOD) activity was significantly increased (P<0.05). In conclusion, the hydroxyethyl-β-cyclodextrin as hyperoside coating material can provide the best solubility, and improve the in vitro antioxidant activity of hyperoside-hydroxyethyl-β-cyclodextrin inclusion complex.

Cite this article

ZHANG Xinyu , SU Jianqing , CHU Xiuling . Preparation of Hyperoside-Cyclodextrin Inclusion Complex and Its Biological Antioxidant Activity[J]. Chinese Journal of Animal Nutrition, 2022 , 34(8) : 5426 -5440 . DOI: 10.3969/j.issn.1006-267x.2022.08.062

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