RESEARCH PAPER

Effects of Adding Acid Xylanase in Wheat-Type Diet on Viscosity of Digestive Tract Chyme of Broilers

  • HUANG Zhiyi ,
  • LIANG Weifan ,
  • LI Yangyuan ,
  • FENG Dingyuan
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  • 1. Guangdong VTR Biotechnology Co., Ltd., Zhuhai 519000, China;
    2. College of Animal Science and Technology, South China Agricultural University, Guangzhou 510642, China

Received date: 2022-03-13

  Online published: 2022-10-17

Abstract

The purpose of this experiment was to compare the enzymatic properties and their viscosity-reducing effects of acidic and neutral xylanases in wheat-type diets. The tolerance of two xylanases (acidic and neutral xylanases) to gastrointestinal fluid was detected by feeding trail; the relative activities of two xylanases were determined under pH 3.5 condition; the viscosity reduced effect of two xylanases was tested using pure xylans and cereals. A total of 512 one-day-old 817 white-feathered broilers were randomly divided into 4 groups (8 replicates in each group and 16 broilers in each replicate), in which the control group were fed the wheat-type basal diet, and the experimental group was supplemented with 4 U/g neutral xylanase, 2 and 4 U/g acidic xylanase, respectively. The test period was 42 days. The results showed as follows:1) compared with neutral xylanase, acidic xylanase had better tolerance to broiler gastrointestinal fluid. 2) Under the condition of pH 3.5, the relative activity of acidic xylanase was 64.1% higher than that of neutral xylanase. 3) For high viscosity (rye) arabinoxylan, acidic xylanase had a 42.9% lower viscosity than neutral xylanase. 4) Adding acid xylanase in the wheat-type diet improved the growth performance of broilers, and 4 U/g acidic xylanase has better effect than 4 U/g neutral xylanase. 5) For broilers fed the wheat-type diet, the crude protein apparent digestibility of 4 U/g acidic xylanase group was increased by 3.5% (P<0.05), 7.4% (P<0.05) and 7.9% (P<0.05) compared with 2 U/g acidic xylanase group, 4 U/g neutral xylanase group and control group, respectively. 6) Adding 4 U/g acidic xylanase in the wheat-type diet significantly reduced chyme viscosity in gizzard, jejunum and ileum of broilers compared with 4 U/g neutral xylanase group and control group (P<0.05). 7) Adding 4 U/g acidic xylanase in the wheat-type diet significantly increased the activities of pepsin and trypsin of broilers compared with control group (P<0.05). It can be seen from the results that the acidic xylanase with higher enzyme activity under the condition of pH 3.5, is more resistant to the gastrointestinal fluid of animals, and can better reduce the viscosity; adding 4 U/g acidic xylanase in the wheat-type diet can improve the growth performance, crude protein apparent digestibility, chyme viscosity and digestive enzyme activities of broilers, and its effect is better than that of neutral xylanase at the same dose.

Cite this article

HUANG Zhiyi , LIANG Weifan , LI Yangyuan , FENG Dingyuan . Effects of Adding Acid Xylanase in Wheat-Type Diet on Viscosity of Digestive Tract Chyme of Broilers[J]. Chinese Journal of Animal Nutrition, 2022 , 34(9) : 5667 -5679 . DOI: 10.3969/j.issn.1006-267x.2022.09.022

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