RESEARCH PAPER

Effects of Different Feeding Methods of Lactic Acid Bacteria on Growth Performance, Crude Protein Apparent Metabolic Rate and Intestinal Health of Broilers

  • CHENG Peng ,
  • ZHANG Jingbo ,
  • ZHAO Longmei ,
  • DING Ke ,
  • CAO Pinghua ,
  • MA Yanbo ,
  • LI Wang
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  • College of Animal Science and Technology, Henan University of Science and Technology, Luoyang 471023, China

Received date: 2022-03-09

  Online published: 2022-10-17

Abstract

This experiment was conducted to study the effects of different feeding methods of lactic acid bacteria on growth performance, crude protein apparent metabolic rate and intestinal health of broilers, and to explore the optimal feeding method of lactic acid bacteria on broilers. A total of 192 one-day-old Arbor Acres (AA) broilers were selected and randomly divided into 4 groups with 6 replicates in each group and 8 chickens in each replicate. The control group (CON group) was fed a basal diet; the mixing group (MIX group) was fed the basal diet supplemented with lactic acid bacteria liquid; the orally administered group (OI group) was fed a basal diet, and orally administered lactic acid bacteria liquid; the drinking water group (DW group) was fed a basal diet, and supplemented lactic acid bacteria liquid in drinking water. The viable bacteria count of lactic acid bacteria liquid was 4×109 CFU/mL. The experiment lasted for 42 days. The results showed as follows:1) during 1 to 42 days of age, the average daily weight gain of DW group and MIX group was significantly higher than that of CON group (P<0.05), and the ratio of feed to gain of MIX group, OI group and DW group was significantly lower than that of CON group (P<0.05). 2) At 21 and 42 days of age, the crude protein apparent metabolic rate of MIX group, OI group and DW group was significantly higher than that of CON group (P<0.05). 3) At 21 days of age, the jejunal villus height, villus surface area and villus height to crypt depth ratio of MIX group, OI group and DW group were significantly higher than those of CON group (P<0.05), and the crypt depth was significantly lower than that of CON group (P<0.05); the ileal villus surface area and villus height to crypt depth ratio of MIX group and DW group were significantly higher than those of CON group (P<0.05). At 42 days of age, the jejunal and ileal villus height and villus height to crypt depth ratio of DW group were significantly higher than those of other groups (P<0.05).4) In each group, Firmicutes and Bacteroidetes were the main microbe in cecum. At 21 and 42 days of age, the cecal Bifidobacterium number of MIX group, OI group and DW group was significantly higher than that of CON group (P<0.05), and the Escherichia coli number was significantly lower than that of CON group (P<0.05). In conclusion, the three feeding methods of lactic acid bacteria can improve the growth performance and crude protein apparent metabolic rate of broilers, and improve the intestinal morphology and intestinal microbial structure, therefore, the effect of feeding with drinking water is better.

Cite this article

CHENG Peng , ZHANG Jingbo , ZHAO Longmei , DING Ke , CAO Pinghua , MA Yanbo , LI Wang . Effects of Different Feeding Methods of Lactic Acid Bacteria on Growth Performance, Crude Protein Apparent Metabolic Rate and Intestinal Health of Broilers[J]. Chinese Journal of Animal Nutrition, 2022 , 34(9) : 5680 -5690 . DOI: 10.3969/j.issn.1006-267x.2022.09.023

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