RESEARCH PAPER

Effects of Lactobacillus reuteri Peptidoglycan on Growth Performance, Immune Function and Antioxidant Capacity of Egg Chicks

  • CHEN Xiaoyu ,
  • ZHU Lianqin ,
  • CHEN Fu ,
  • ZHANG Beibei ,
  • ZHU Fenghua
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  • 1. Department of Animal Medicine, Qingdao Agricultural University, Qingdao 266109, China;
    2. Department of Animal Science, Qingdao Agricultural University, Qingdao 266109, China

Received date: 2022-03-13

  Online published: 2022-10-17

Abstract

The purpose of this experiment was to study the effects of dietary Lactobacillus reuteri peptidoglycan on growth performance, immune function and antioxidant capacity of egg chicks. Ninety-six healthy 1-day-old Hy-Line Brown egg chicks were randomly divided into 4 groups with 4 replicates in each group and 6 chicks in each replicate. Chicks in the control group were fed a basal diet, and others in experimental groups were fed basal diets supplemented 250, 500 and 1 000 mg/kg Lactobacillus reuteri peptidoglycan, respectively. The experiment lasted for 42 days. The results showed as follows:1) on days 21 and 42 of the experiment, the average daily gain of 1 000 mg/kg Lactobacillus reuteri peptidoglycan group was significantly higher than that of other groups (P<0.05), and the feed to gain ratio of 1 000 mg/kg Lactobacillus reuteri peptidoglycan group was significantly lower than that of the control group (P<0.05). 2) On day 42 of the experiment, the white blood cell count of 500 and 1 000 mg/kg Lactobacillus reuteri peptidoglycan groups was significantly higher than that of control group and 250 mg/kg Lactobacillus reuteri peptidoglycan group (P<0.05). 3) On days 21 and 42 of the experiment, the activities of glutathione peroxidase (GSH-Px) and superoxide dismutase (SOD) in plasma of 1 000 mg/kg Lactobacillus reuteri peptidoglycan group were significantly higher than those of the control group (P<0.05), and the plasma total antioxidant capacity (T-AOC) of 500 and 1 000 mg/kg Lactobacillus reuteri peptidoglycan groups was significantly higher than that of control group and 250 mg/kg Lactobacillus reuteri peptidoglycan group (P<0.05). 4) On day 42 of the experiment, the plasma interleukin-2 (IL-2) content of 500 and 1 000 mg/kg Lactobacillus reuteri peptidoglycan groups was significantly higher than that of the control group (P<0.05), and the contents of immunoglobulin A (IgA), immunoglobulin G (IgG), interleukin-4 (IL-4), interleukin-6 (IL-6) and interferon-γ (IFN-γ) in plasma of 1 000 mg/kg Lactobacillus reuteri peptidoglycan group were significantly higher than those of control group and 250, 500 mg/kg Lactobacillus reuteri peptidoglycan groups (P<0.05). 5) The levels of Newcastle disease virus antibody (NDV-Ab) and infectious bursal disease virus antibody (IBDV-Ab) in plasma of 1 000 mg/kg Lactobacillus reuteri peptidoglycan group were significantly higher than those of control group and 250, 500 mg/kg Lactobacillus reuteri peptidoglycan groups (P<0.05). 6) The spleen index of 1 000 mg/kg Lactobacillus reuteri peptidoglycan group was significantly higher than that of control group and 250, 500 mg/kg Lactobacillus reuteri peptidoglycan groups (P<0.05), and the thymus index of 250 and 1 000 mg/kg Lactobacillus reuteri peptidoglycan groups was significantly higher than that of control group and 500 mg/kg Lactobacillus reuteri peptidoglycan group (P<0.05). In conclusion, dietary Lactobacillus reuteri peptidoglycan can improve the growth performance of egg chicks, promote the development of immune organs, and improve the body immune function and antioxidant capacity. It is feasible to add 1 000 mg/kg Lactobacillus reuteri peptidoglycan in the diet of egg chicks.

Cite this article

CHEN Xiaoyu , ZHU Lianqin , CHEN Fu , ZHANG Beibei , ZHU Fenghua . Effects of Lactobacillus reuteri Peptidoglycan on Growth Performance, Immune Function and Antioxidant Capacity of Egg Chicks[J]. Chinese Journal of Animal Nutrition, 2022 , 34(9) : 5741 -5750 . DOI: 10.3969/j.issn.1006-267x.2022.09.029

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