RESEARCH PAPER

Effects of Seabuckthorn Flavonoids on Rumen Fermentation Parameters, Rumen Microorganism Structure and Metabolites in Mid-Lactation Holstein Dairy Cows

  • ZHAO Huiying ,
  • YU Shiqiang ,
  • HE Liying ,
  • XIONG Benhai ,
  • WANG Hui ,
  • JIANG Linshu
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  • 1. Beijing Key Laboratory of Dairy Cow Nutrition, College of Animal Science and Technology, Beijing University of Agriculture, Beijing 102206, China;
    2. Beijing Institute of Animal Science and Veterinary Medicine, Chinese Academy of Agricultural Sciences, Beijing 100193, China

Received date: 2022-03-09

  Online published: 2022-10-17

Abstract

This experiment was conducted to study the effects of seabuckthorn flavonoids on rumen fermentation parameters, rumen microorganism structure and metabolites in mid-lactation Holstein dairy cows. Thirty Chinese Holstein dairy cows with similar parity, body weight and daily milk yield and days in milk were randomly divided into 3 groups with 10 cows per group. Seabuckthorn flavonoids were top-dressed 0 (group BC), 60 (group BL) and 100 g/(d·head) (group BM) to the total mixed ration (TMR), respectively. The experiment lasted for 75 days, the pre-trial period lasted for 15 days and the formal trial period lasted for 60 days. The results showed as follows:1) compared with group BC, the concentration of acetate and the ratio of acetate to propionate in rumen fluid in group BL significantly increased (P<0.05), and the concentration of valerate in groups BL and BM significantly decreased (P<0.05). 2) At the phylum level, compared with group BC, adding seabuckthorn flavonoids had no significant effect on the relative abundance of rumen bacteria (P>0.05). At the genus level, compared with group BC, the relative abundances of Bacteroidales_RF16_group, Rikenellaceae_RC9_gut_group and Ruminiclostridium in group BL significantly increased (P<0.05). 3) Compared with group BC, the contents of 19 metabolites (phenylpyruvic acid, anthranilic acid, bergenin, nifenazone, pelargonidin, rutin and so on) in group BL significantly up-regulated (P<0.05), and the contents of 6 metabolites[(2S)-2-amino-5-oxopentanoic acid, 3-dehydroquinic acid, D-lyxose, o-phosphohomoserine, 5,6-dihydroxycyclohexa-1,3-diene-1-carboxylate and N-acetylpuromycin] significantly down-regulated (P<0.05). According to KEGG functional annotation and enrichment analysis, differential metabolites were mainly enriched in phenylalanine, tyrosine and tryptophan biosynthesis pathway and amino acid biosynthesis pathway. In conclusion, dietary supplementation of seabuckthorn flavonoids increase the concentration of acetate and the ratio of acetate to propionate, and decrease the concentration of valeratein rumen of mid-lactation Holstein dairy cows. It also increases the relative abundance of fibre-degrading bacteria. Flavonoids from sea buckthorn increases the contents of metabolites related to amino acid biosynthesis pathways in rumen.

Cite this article

ZHAO Huiying , YU Shiqiang , HE Liying , XIONG Benhai , WANG Hui , JIANG Linshu . Effects of Seabuckthorn Flavonoids on Rumen Fermentation Parameters, Rumen Microorganism Structure and Metabolites in Mid-Lactation Holstein Dairy Cows[J]. Chinese Journal of Animal Nutrition, 2022 , 34(9) : 5809 -5823 . DOI: 10.3969/j.issn.1006-267x.2022.09.037

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