Effects of Supplementary Feeding with Chromium Methionine in Summer on Heat Stress State, Antioxidant Capacity and Passive Immune Transfer Ability of Dry Cows

MA Yixuan; SHEN Yizhao; LI Yan; FENG Wei; ZHANG Haibo; GUO Gang; WANG Meimei; SUN Fengli; LI Qiufeng; LI Jianguo; CAO Yufeng; GAO Yanxia

doi:10.3969/j.issn.1006-267x.2022.09.040

2022 , Vol. 34 >Issue 9: 5852 - 5865

DOI: https://doi.org/10.3969/j.issn.1006-267x.2022.09.040

RESEARCH PAPER

Effects of Supplementary Feeding with Chromium Methionine in Summer on Heat Stress State, Antioxidant Capacity and Passive Immune Transfer Ability of Dry Cows

MA Yixuan ,
SHEN Yizhao ,
LI Yan ,
FENG Wei ,
ZHANG Haibo ,
GUO Gang ,
WANG Meimei ,
SUN Fengli ,
LI Qiufeng ,
LI Jianguo ,
CAO Yufeng ,
GAO Yanxia

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1. College of Animal Science and Technology, Hebei Agricultural University, Baoding 071001, China;
2. College of Veterinary Medicine, Hebei Agricultural University, Baoding 071001, China;
3. Beijing Shounong Animal Husbandry Development Co., Ltd., Beijing 100076, China;
4. Hebei Institute of Animal Science and Veterinary Medicine, Baoding 071000, China;
5. Embryo Engineering and Technological Center of Cattle and Sheep of Hebei, Baoding 071001, China;
6. Hebei Dairy Industry Technology Research Institute, Shijiazhuang 050000, China

Received date: 2022-03-13

Online published: 2022-10-17

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Abstract

This experiment was conducted to study the effects of chromium methionine on rectal temperature, antioxidant capacity and passive immune transfer ability of dry cows under heat stress, and to provide a theoretical basis for the rational application of chromium methionine in dry cows. Seventy-two healthy Holstein dry cows with similar parity, pre-natal period and body weight were selected in the experiment. The single-factor completely randomized experimental design was adopted and they were randomly divided into four groups (groups C, L, M and H), with 18 replicates in each group and one cow in each replicate. The dry cows in control group (group C) were fed a pasture-basal diet, and those in groups L, M and H were supplemented with chromium methionine (Cr-Met) at an effective chromium content of 3, 6 and 9 mg/(head·d) in the basal diet, respectively. The experimental period was from the 60th day before the expected calving to the day of calving. Rectal temperature was measured every 10 days during the experiment. Blood samples of dry cows were collected from tail vein on the 60th, 28th and 10th day of prenatal delivery and the day of calving, and the serum biochemical, antioxidant and immune indexes were determined. Colostrum samples were collected immediately after calving, and colostrum was infused within 2 hours after birth. Blood samples of cavies were collected from jugular vein 24 hours later, and blood immune indexes were determined. The results showed as follows:1) the rectal temperature of dry cows at 15:00 on each period was not significantly affected by dietary Cr-Met supplementation (P>0.05), but the rectal temperature at 09:00 on the 30th day before delivery decreased linearly with the increase of Cr-Met supplemental level (P=0.02). On the 10th day before delivery, the rectal temperature of dry cows at 15:00 in groups L and H was significantly lower than that in control group (P<0.05). During the whole period, the rectal temperature of dry cows at 09:00 in groups M and group H was significantly lower than that in control group (P<0.05). 2) On the 45th day before delivery, the dry matter intake (DMI) of dry cows in Cr-Met supplementation groups was significantly higher than that in control group (P<0.05). 3) During the whole period, the serum total antioxidant capacity (T-AOC) of dry cows in group M was significantly higher than that in control group (P<0.05), and the serum superoxide dismutase (SOD) activities in groups L and M was significantly higher than that in control group (P<0.05). On the day of delivery, the serum malondialdehyde (MDA) content of dry cows decreased cubically with the increase of Cr-Met supplemental level (P=0.04). 4) On the 10th day before delivery, the serum triglyceride (TG) content of dry cows in group M was significantly lower than that in other groups (P<0.05). On the 28th day before delivery, the content of serum non-esterified fatty acids (NEFA) in group M was significantly lower than that in control group (P<0.05). On the 10th day before delivery, the content of serum NEFA in groups L and M was significantly lower than that in control group (P<0.05), and the content of serum β-hydroxybutyrate (BHBA) shows a quadratic decrease trend with the increase of Cr-Met supplemental level (P=0.08). 5) The content of immunoglobulin G (IgG) in colostrum in group M was significantly higher than that in control group (P<0.05). The content of immunoglobulin A (IgA) in colostrum showed a linear increase trend with the increase of Cr-Met supplemental level (P=0.08). The content of immunoglobulin M (IgM) in group M was significantly higher than that in the control group and group H (P<0.05). On the 28th day before delivery, the serum IgA content of dry cows increased quadratically with the increase of Cr-Met supplemental level (P=0.04), and the IgM content of dry cows in groups L, M and H was significantly higher than that in control group (P<0.05). On the 10th day before delivery, the serum IgM content of dry cows tended to increase quadratically with the increase of Cr-Met supplemental level (P=0.09). On the day of delivery, the serum IgG content of dry cows tended to increased linearly with the increase of Cr-Met supplemental level (P=0.07), and the IgM content tended to increase quadratically (P=0.09). The serum IgG content in group M was significantly higher than that in control group and group H (P<0.05). In conclusion, dietary supplementation of Cr-Met in dry cows can effectively alleviate heat stress, improve antioxidant capacity and passive immune transfer, and the effect of Cr-Met with effective chromium content of 6 mg/(head·d) is the best.

Key words： dry cows; chromium methionine; heat stress; antioxidation; passive immune transfer

Cite this article

MA Yixuan , SHEN Yizhao , LI Yan , FENG Wei , ZHANG Haibo , GUO Gang , WANG Meimei , SUN Fengli , LI Qiufeng , LI Jianguo , CAO Yufeng , GAO Yanxia . Effects of Supplementary Feeding with Chromium Methionine in Summer on Heat Stress State, Antioxidant Capacity and Passive Immune Transfer Ability of Dry Cows[J]. Chinese Journal of Animal Nutrition, 2022 , 34(9) : 5852 -5865 . DOI: 10.3969/j.issn.1006-267x.2022.09.040

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