RESEARCH PAPER

Effects of Lipid Levels in High Dietary Fibre Diets on Growth, Liver and Intestine Health of Juvenile Largemouth Bass (Micropterus salmoides)

  • SHI Ye ,
  • CAI Chunfang ,
  • KANG Jia ,
  • HU Honglin ,
  • CAO Xiamin ,
  • WU Ping ,
  • YE Yuantu ,
  • DING Huiming
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  • 1. School of Biology and Basic Medical Science, School of Soochow University, Suzhou 215123, China;
    2. Key Laboratory of Aquatic Animal Nutrition, School of Soochow University, Suzhou 215123, China;
    3. Suzhou Yangchenghu National Modern Agriculture Demonstration Zone Development Co., Ltd., Suzhou 215138, China

Received date: 2022-03-30

  Online published: 2022-10-17

Abstract

The content of dietary fibre (DF) was high in farmed fish feed because of the wide use of plant feed materials, which was found to be contributed to enteritis and fatty liver. This study was to verify the effects of lipid levels in high DF diets on the growth, liver and intestinal health of largemouth bass (Micropterus salmoides). A total of 120 largemouth bass (initial body weight:10.6 g/fish) were randomly divided into 2 groups with 3 replicates per group and 20 largemouth bass per replicate. Both of two diets were supplemented with 15% guar gum, soybean oil was added in 3% (LL group, measured level was 12.81%) and 8% (HL group, measured level was 17.94%), respectively. Diets were fed two times a day. Feeding period was 56 days. The results showed as follows:1) weight gain rate, specific growth rate and condition factor were significantly higher in the HL group than the LL group (P<0.05), with no significant differences in body composition or liver lipid content between the two groups (P>0.05). 2) The HL group showed a significant decrease in serum glutaminase activity and liver collagen content compared with the LL group (P<0.05). 3) Compared with LL group, the mRNA relative expression levels of the lipid metabolism-related genes carnitine palmitoyltransferase 1 (CPT1), peroxisome proliferators-activated receptor α (PPARα), apolipoprotein A1 (APOA1) and the inflammatory gene transforming growth factor β (TGFβ) was significantly upregulated in the liver (P<0.05) and the mRNA relative expression level of the inflammatory gene nuclear factor-kappa β (NF-κβ) was significantly decreased (P<0.05). In the intestine, the mRNA relative expression levels of NF-κβ, tumor necrosis factor α (TNFα), interleukin-10 (IL-10) and TGFβ were significantly down-regulated in the HL group compared with the LL group (P<0.05). 4) Histological analysis showed that the liver fibrosis in HL group were weaker than that in LL group. No obvious damage to the intestinal morphology was noticed in both groups. In conclusion, these results suggest that higher lipid levels in high DF diets benefit to growth and liver and intestinal health of largemouth bass.

Cite this article

SHI Ye , CAI Chunfang , KANG Jia , HU Honglin , CAO Xiamin , WU Ping , YE Yuantu , DING Huiming . Effects of Lipid Levels in High Dietary Fibre Diets on Growth, Liver and Intestine Health of Juvenile Largemouth Bass (Micropterus salmoides)[J]. Chinese Journal of Animal Nutrition, 2022 , 34(9) : 6021 -6032 . DOI: 10.3969/j.issn.1006-267x.2022.09.055

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