RESEARCH PAPER

Effects of Zinc-Bearing Palygorskite on Immunity, Antioxidant Capability and Resistance to Transport Stress of Dogs

  • LUO Youwen ,
  • ZHENG Dan ,
  • CAO Bin ,
  • YUAN Huagen ,
  • CAO Yun ,
  • LI Xue
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  • 1. Jiangsu Animal Husbandry and Veterinary College, Taizhou 225300, China;
    2. Taizhou College of Nanjing Normal University, Taizhou 225300, China

Received date: 2022-03-31

  Online published: 2022-10-17

Abstract

The study was conducted to explore the effects of zinc-bearing palygorskite (Zn-Pal) on serum immunity, antioxidant capability and resistance to transport stress of dogs. Twenty 10-month-old Beagle dogs were randomly divided into 4 groups with 5 dogs in each group. They were fed the basal diet+ZnSO4 (zinc supplemental level was 80 mg/kg, control group), basal diet+1.71 g/kg Zn-Pal (zinc supplemental level was 40 mg/kg, Zn-Pal-40 group), basal diet+2.55 g/kg Zn-Pal (zinc supplemental level was 60 mg/kg, Zn-Pal-60 group) and basal diet+3.42 g/kg Zn-Pal (zinc supplemental level was 80 mg/kg, Zn-Pal-80 group), respectively. The pre-trial period was 7 days, and the formal trial period was 30 days. At the end of the feeding test, dogs were caged and transported at a speed of about 60 km/h for 2 h. Serum immune, antioxidant-related indexes and acute phase reaction biomarker contents of dogs before and after transportation were determined. The results showed that, before transport stress, the serum interleukin-6 (IL-6) content in Zn-Pal-60 and Zn-Pal-80 groups was significantly higher than that in control group (P<0.05), and the serum immunoglobulin G (IgG) content in Zn-Pal-80 group was significantly higher than that in control group (P<0.05); the serum total antioxidant capacity (T-AOC) and total superoxide dismutase (T-SOD) activity in Zn-Pal-80 group were significantly higher than that in control group (P<0.05), and the serum malondialdehyde (MDA) content in Zn-Pal-60 and Zn-Pal-80 groups was decreased compared with control group (P>0.05); the serum cortisol content in Zn-Pal-60 and Zn-Pal-80 groups was decreased compared with control group (P>0.05). After transport stress, the serum T-SOD activity in Zn-Pal-80 group increased significantly and the serum MDA content decreased significantly compared with control group (P<0.05); the serum T-SOD activity and MDA content was increased and decreased compared with control group, respectively (P>0.05); the serum cortisol content in Zn-Pal-60 and Zn-Pal-80 groups was significantly lower than that in control group (P<0.05), and the serum heat shock protein 70 (HSP70) content in Zn-Pal-80 group was significantly lower than that in control group (P<0.05). In conclusion, Zn-Pal can increase the immunity, antioxidant capability, and improve the resistance to transport stress of dogs.

Cite this article

LUO Youwen , ZHENG Dan , CAO Bin , YUAN Huagen , CAO Yun , LI Xue . Effects of Zinc-Bearing Palygorskite on Immunity, Antioxidant Capability and Resistance to Transport Stress of Dogs[J]. Chinese Journal of Animal Nutrition, 2022 , 34(9) : 6064 -6071 . DOI: 10.3969/j.issn.1006-267x.2022.09.059

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