RESEARCH PAPER

Effects of Fecal Microbiota Transplantation of Free Range and Captive Saba Pigs on Fat Deposition and Fecal Microflora in Mice

  • HU Tiannian ,
  • CHENG Yandong ,
  • YIN Huajuan ,
  • CHA Guanghui ,
  • WANG Pu ,
  • PAN Qiong ,
  • YE Pengfei ,
  • LIN Qiuye ,
  • CAO Zhenhui
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  • 1. Faculty of Animal Science and Technology, Yunnan Agricultural University, Kunming 650201, China;
    2. College of Food Science and Technology, Yunnan Agricultural University, Kunming 650201, China

Received date: 2022-03-22

  Online published: 2022-10-17

Abstract

This study aimed to investigate the effects of fecal microbiota transplantation (FMT) of free range and captive Saba pigs on fat deposition and fecal microflora in Kunming mice. Healthy Saba pigs under either free range or intensively farmed conditions were selected as FMT donors to prepare fecal microbiota solution. Then thirty healthy male Kunming mice were selected as FMT recipients and randomly divided into 5 groups with 6 mice in each group. The control (CON) group was intragastrically fed with normal saline, and the experimental groups were intragastrically fed with low-dose FMT bacteria solution of free range pigs (SY-L group), medium-dose FMT bacteria solution of free range pigs (SY-M group), high-dose FMT bacteria solution of free range pigs (SY-H group) and medium-dose FMT bacteria solution of captive pigs (JY-M group) for 14 consecutive days, respectively. The experiment lasted for 28 days. The results showed as follows:1) compared with the CON group, the average daily gain (ADG) of mice in the experimental groups was increased, and the feed to gain ratio (F/G) was decreased, in which the differences were significant in SY-M group (P<0.05). Compared with JY-M group, ADG in SY-M group was significantly increased (P<0.05), and F/G was significantly decreased (P<0.05). 2) Compared with the CON group, the epididymal fat index, groin fat index and total fat index of mice in the experimental groups were decreased, and significant differences were found in SY-M group (P<0.05). Compared with JY-M group, the epididymal fat index, groin fat index and total fat index in SY-M group were decreased by 12.32%, 10.61% and 11.76%, respectively (P>0.05). 3) Compared with the CON group, the relative abundance of Firmicutes and Firmicutes/Bacteroides in feces of mice in the experimental groups were decreased, while the relative abundances of Bacteroides (except SY-H group) and Lactobacillus were increased, among which the relative abundance of Lactobacillus in SY-M group was the highest and the difference was significant (P<0.05). There was no significant difference in the above indices between SY-M group and JY-M group (P>0.05). 4) The correlation analysis showed that the relative abundance of Lactobacillus in feces of mice in SY-M group was negatively correlated with the epididymal fat index, groin fat index and total fat index. In conclusion, FMT of free range and captive Saba pigs can increase intestinal flora diversity and relative abundance of Lactobacillus, reduce Firmicutes/Bacteroides, thereby promoting growth and reducing fat deposition in mice, among which FMT of free range Saba pigs has the best effect in medium dose.

Cite this article

HU Tiannian , CHENG Yandong , YIN Huajuan , CHA Guanghui , WANG Pu , PAN Qiong , YE Pengfei , LIN Qiuye , CAO Zhenhui . Effects of Fecal Microbiota Transplantation of Free Range and Captive Saba Pigs on Fat Deposition and Fecal Microflora in Mice[J]. Chinese Journal of Animal Nutrition, 2022 , 34(9) : 6097 -6109 . DOI: 10.3969/j.issn.1006-267x.2022.09.062

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