RESEARCH PAPER

Effects of Dietary Enterococcus faecalis on Growth Performance, Nitrogen Metabolism, Serum Biochemical Indices and Fecal Microbiota of Growing Ussuri Raccoon Dogs

  • BAI Yinlong ,
  • NIU Yibing ,
  • ZHANG Haihua ,
  • GONG Wenyang ,
  • QU Yicheng ,
  • LONG Juan ,
  • WANG Xiguo ,
  • GONG Yuanfang ,
  • ZHAO Huiyuan ,
  • LI Sufen ,
  • FENG Minshan
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  • 1. Hebei Normal University of Science and Technology, Qinhuangdao 066004, China;
    2. Qinhuangdao Sanjing Xinnong Feed Co., Ltd., Changli 066600, China;
    3. Hebei Jieshi Fur Industry Research Institute, Changli 066600, China

Received date: 2022-02-26

  Online published: 2022-10-17

Abstract

This experiment was conducted to investigate the effects of different dietary levels of Enterococcus faecalis on the growth performance, nitrogen metabolism, serum biochemical indices and fecal microbiota of growing Ussuri raccoon dogs, so as to determine the optimal dietary level of Enterococcus faecalis and its mechanism. A total of 40 male healthy Ussuri raccoon dogs of 70-day-old with similar body weight of (2.44±0.24) kg were randomly divided into 4 groups with 10 replicates per group, and fed the basal diet (control group) or the basal diet supplemented with 1×108 (group Ⅰ), 1×109 (group Ⅱ) and 1×1010 CFU/kg (group Ⅲ) Enterococcus faecalis, respectively. The trial lasted for 70 days after a 7-day adaption. The results showed as follows: 1) compared with the control group, the average daily gain in groups Ⅰ and Ⅱ was significantly increased (P < 0.05), and which in group Ⅰ was significantly higher than that in group Ⅱ (P < 0.05); the feed to gain ratio in groups Ⅰ and Ⅱ was significantly decreased (P < 0.05); there were no significant differences in the above two indices in group Ⅲ (P>0.05). 2) Compared with the control group, the nitrogen deposition in all Enterococcus faecalis supplemental groups and the protein biological value in groups Ⅰ and Ⅱ were significantly increased (P < 0.05). 3) Compared with the control group, the serum albumin content in groups Ⅰ and Ⅱ was significantly increased (P < 0.05), and the serum urea nitrogen content in the three Enterococcus faecalis supplemental groups was significantly increased (P < 0.05). 4) Compared with the control group, the ACE index and Chao1 index of fecal microbiota in group Ⅰ were significantly increased (P < 0.05), the relative abundances of Bacteroidetes and Megasphaera were significantly increased (P < 0.05), and the relative abundances of Dialister, Catenisphaera and Mitsuokella were significantly decreased (P < 0.05). The results suggested that dietary Enterococcus faecalis can improve the growth performance and nitrogen utilization of growing Ussuri raccoon dogs, promote beneficial bacteria and inhibit potential pathogens; the optimal dietary level is 1×108 CFU/kg.

Cite this article

BAI Yinlong , NIU Yibing , ZHANG Haihua , GONG Wenyang , QU Yicheng , LONG Juan , WANG Xiguo , GONG Yuanfang , ZHAO Huiyuan , LI Sufen , FENG Minshan . Effects of Dietary Enterococcus faecalis on Growth Performance, Nitrogen Metabolism, Serum Biochemical Indices and Fecal Microbiota of Growing Ussuri Raccoon Dogs[J]. Chinese Journal of Animal Nutrition, 2022 , 34(10) : 6687 -6694 . DOI: 10.3969/j.issn.1006-267x.2022.10.061

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