Alleviation Effect of Soybean Bioactive Peptide on Oxidative Stress and Inflammatory Injury of Bovine Mammary Epithelial Cells Induced by Hydrogen Peroxide in Vitro

CHEN Yi; ZHANG Yihan; ZHANG Jing; TU Yan; WANG Hui; JIANG Linshu

doi:10.3969/j.issn.1006-267x.2022.10.064

2022 , Vol. 34 >Issue 10: 6714 - 6725

DOI: https://doi.org/10.3969/j.issn.1006-267x.2022.10.064

RESEARCH PAPER

Alleviation Effect of Soybean Bioactive Peptide on Oxidative Stress and Inflammatory Injury of Bovine Mammary Epithelial Cells Induced by Hydrogen Peroxide in Vitro

CHEN Yi ,
ZHANG Yihan ,
ZHANG Jing ,
TU Yan ,
WANG Hui ,
JIANG Linshu

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1. College of Animal Science and Technology, Beijing University of Agricultural, Beijing 102206, China;
2. Feed Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China

Received date: 2022-04-13

Online published: 2022-10-17

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Abstract

This experiment was conducted to investigate the alleviation effect of soybean bioactive peptide (SBP) on oxidative stress and inflammatory injury of bovine mammary epithelial cells (MAC-T) induced by hydrogen peroxide (H₂O₂). In vitro cell culture method was used in this experiment. Based on the oxidative stress model of MAC-T cells established by different concentrations of H₂O₂, the cell viability, and the level of reactive oxygen (ROS), antioxidant indexes and the mRNA expression levels of genes related to inflammation in cells were measured after treatment with different concentrations (0.15, 0.30, 0.60, 1.20, 2.40 and 4.80 mg/mL) of SBP, a control group and a H₂O₂ model group were set at the same time. The results showed that compared with the control group, the intracellular ROS level was extremely significantly increased after 600 μmol/L H₂O₂ treatment for 12 h (P < 0.01), and the activities of total superoxide dismutase (T-SOD), glutathione peroxidase (GSH-Px), catalase (CAT) and total antioxidant capacity (T-AOC) were extremely significantly decreased (P < 0.01), while the content of MDA was extremely significantly increased (P < 0.01). After 0.15, 0.30 and 0.60 mg/mL SBP pretreatment on cells, compared with H₂O₂ model group, intracellular ROS level was extremely significantly decreased (P < 0.01), the T-SOD, GSH-Px and CAT activities were extremely significantly increased (P < 0.01), the T-AOC were significantly or extremely significantly increased (P < 0.05 or P < 0.01), the MDA content was extremely significantly reduced (P < 0.01), and the mRNA relative expression levels of intracellular inflammatory mediators [cyclooxygenase-2 (COX-2), inducible nitric oxide synthase (iNOS) and monocyte chemotactic protein-1 (MCP-1)] and pro-inflammatory factors [tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), interleukin-8 (IL-8) and interleukin-1β (IL-1β)] were extremely significantly decreased (P < 0.01). In conclusion, in vitro, SBP relieves oxidative stress and inflammatory damage of MAC-T cells induced by H₂O₂ may through reducing intracellular ROS level, improving the activities of antioxidant enzymes, and regulating the expression of inflammation-related genes.

Key words： soybean bioactive peptide; bovine mammary epithelial cells; oxidative stress; inflammatory injury

Cite this article

CHEN Yi , ZHANG Yihan , ZHANG Jing , TU Yan , WANG Hui , JIANG Linshu . Alleviation Effect of Soybean Bioactive Peptide on Oxidative Stress and Inflammatory Injury of Bovine Mammary Epithelial Cells Induced by Hydrogen Peroxide in Vitro[J]. Chinese Journal of Animal Nutrition, 2022 , 34(10) : 6714 -6725 . DOI: 10.3969/j.issn.1006-267x.2022.10.064

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