RESEARCH PAPER

Polysaccharide of Atractylodes macrocephala Koidz Alleviates Lipopolysaccharide Induced Bursa of Fabricius Injury in Goslings through Toll-Like Receptor 4/p38 Mitogen Activated Protein Kinase Signaling Pathway

  • ZHANG Bingqi ,
  • LI Bingxin ,
  • YANG Baohe ,
  • YUAN Mingfeng ,
  • LI Wanyan ,
  • CAO Nan ,
  • XU Danning ,
  • TIAN Yunbo ,
  • CHEN Wenbin
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  • 1. College of Animal Science and Technology, Zhongkai University of Agricultural Engineering, Guangzhou 510225, China;
    2. Guangdong Province Key Laboratory of Waterfowl Healthy Breeding, Guangzhou 510225, China;
    3. Yunnan Kuoduo Animal Husbandry Technology Co., Ltd., Yuxi 653100, China;
    4. College of Animal Science and Technology, Hunan Agricultural University, Changsha 410128, China

Received date: 2022-02-18

  Online published: 2022-10-17

Abstract

The purpose of this study was to investigate the effects of polysaccharide of Atractylodes macrocephala Koidz (PAMK) on lipopolysaccharide (LPS)-induced bursa of Fabricius injury in goslings. A total of 200 one-day-old goslings (half male and half female) were selected and randomly divided into control group, LPS group, PAMK group and PAMK+LPS group. The contents of PAMK in each group was 0, 0, 400 and 400 mg/kg, respectively. There were 5 replicates in each group and 10 replicates in each replicate. The goslings in the LPS group and PAMK+LPS group were injected with LPS at a concentration of 2 mg/kg by intraperitoneal injection at 24, 26 and 28 days of age, respectively, and the control group and PAMK group were injected with equal volume of normal saline. One hour after injection at the age of 28 days, 15 goslings in each group were randomly selected to collect bursa of Fabricius for morphological and histological observation, detection of immune cytokines and Toll-like receptor 4 (TLR4) signaling pathway-related gene expression and oxidative stress-related indicators. The results showed that PAMK could effectively relieve LPS-induced disorder of cell arrangement in bursal vesicles, increase in the number of vacuoles in vesicles and decrease in cortex area to medulla area ratio. In addition, PAMK could significantly inhibit LPS-induced up-regulation of tumor necrosis factor-α (TNF-α), interleukin-1 β (IL-1β), interleukin-6 (IL-6) and interleukin-10 (IL-10) mRNA relative expression levels (P < 0.05). The results of TLR4 pathway detection showed that PAMK could significantly alleviate LPS-induced up-regulation of Toll-like receptor 4 (TLR4) and p38 mitogen activated protein kinase (p38 MAPK) mRNA relative expression levels and protein expression levels (P < 0.05). Meanwhile, The PAMK could significantly increase the superoxide dismutase (SOD) activity in the bursa of Fabricius when compared with the LPS group (P < 0.05). These results suggest that PAMK alleviates LPS-induced bursa injury in goslings through the TLR4 signaling pathway.

Cite this article

ZHANG Bingqi , LI Bingxin , YANG Baohe , YUAN Mingfeng , LI Wanyan , CAO Nan , XU Danning , TIAN Yunbo , CHEN Wenbin . Polysaccharide of Atractylodes macrocephala Koidz Alleviates Lipopolysaccharide Induced Bursa of Fabricius Injury in Goslings through Toll-Like Receptor 4/p38 Mitogen Activated Protein Kinase Signaling Pathway[J]. Chinese Journal of Animal Nutrition, 2022 , 34(10) : 6737 -6747 . DOI: 10.3969/j.issn.1006-267x.2022.10.066

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