RESEARCH PAPER

Succession Analysis of Intestinal Microbiota Structure of Mice with Pig Fecal Microbiota Transplantation

  • SONG Yuanyuan ,
  • SHI Weiling ,
  • ZHANG Xiaojun ,
  • YANG Hua ,
  • WU Choufei ,
  • XIAO Yingping
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  • 1. College of Life Science, Huzhou University, Huzhou 313000, China;
    2. State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products, Institute of Agro-Product Safety and Nutrition, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China;
    3. Zhejiang Dovro Animal Health Business Company, Jinhua 321017, China;
    4. Institute of Animal Husbandry and Veterinary Medicine, Jinhua Academy of Agricultural Sciences, Jinhua 321017, China

Received date: 2022-02-26

  Online published: 2022-10-17

Abstract

This experiment was conducted to explore the dynamic succession of the intestinal microbiota structure of mice with pig fecal microbiota transplantation. Jinhua pigs and Landrace pigs were used as fecal transplant donors, and fecal microorganisms from pigs were administered into germ-free mice for 7 days after the treatment of broad spectrum antibiotics. The feces of mice were collected at days 1, 2, 4 and 7 during the administration and days 7, 14, 21 and 28 after the administration. The dynamic changes in the structure and quantity of mice intestinal microbiota and the changes in short-chain fatty acids (SCFAs) contents in the feces were determined by 16S rRNA gene sequencing, RT-PCR, and gas chromatography, respectively. The results showed that the number and alpha diversity of microorganisms in the fecal samples of recipient mice increased with transplantation time during pig fecal microbiota transplantation. At the phylum level, the relative abundance of Firmicutes and Proteobacteria in the intestine of recipient mice gradually decreased over the time of transplantation, while the relative abundance of Bacteroidetes and Verrucomicrobia increased; at the genus level, the relative abundance of Bacteroides, Akkermansia and Parabacteroides increased, while the relative abundance of Lactobacillus, Enterococcus and Escherichia decreased until reaching a stable platform after the transplantation. The content of SCFAs in the fecal samples of mice was gradually increased over the time of transplantation and the changes in the content of SCFAs became less after the transplantation and remained stable at day 21 after the transplantation. The results suggest that cross-species transplantation of pig fecal microbiota into antibiotic-treated mice can lead to dynamic succession in the intestinal intestinal microbiota of the recipient mice, the intestinal microbiota structure of mice with fecal microbiota transplantation from different pig breeds are different due to the influence of pig donor microorganisms and the contents of SCFAs produced by the metabolism of the intestinal microbiota also show a similar pattern of change in the number of intestinal microorganisms.

Cite this article

SONG Yuanyuan , SHI Weiling , ZHANG Xiaojun , YANG Hua , WU Choufei , XIAO Yingping . Succession Analysis of Intestinal Microbiota Structure of Mice with Pig Fecal Microbiota Transplantation[J]. Chinese Journal of Animal Nutrition, 2022 , 34(10) : 6758 -6767 . DOI: 10.3969/j.issn.1006-267x.2022.10.068

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