RESEARCH PAPER

Isolation and Safety Evaluation of a Sheep-Derived Lactobacillus curvatus

  • XIANG Weiwei ,
  • YE Mengjun ,
  • HOU Meng ,
  • YIN Fangfang ,
  • LIU Yawei ,
  • WANG Yinlong ,
  • PAN Rui ,
  • WANG Jinquan
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  • College of Animal Medicine, Xinjiang Agricultural University, Urumqi 830052, China

Received date: 2022-04-07

  Online published: 2022-10-17

Abstract

This study was to evaluate the potential probiotic properties and safety of a sheep-derived Lactobacillus curvatus. Thirteen sheep fecal samples were collected for the isolation of lactic acid bacteria. Then, the pathogenicity test in mice was carried out, mice were then examined to determine the safety of isolated bacteria. The results showed as follows: 1) fifty-five lactic acid bacterial strains (number: Y1 to Y55) were isolated. 2) The isolated bacterium Y15, tolerated pH=3 and 1.0 % bile salt, was susceptible to penicillin G, cefotaxime, ampicillin, clindamycin and tetracycline; and it showed the ability to inhibit Staphylococcus aureus and Escherichia coli without any detectable hemolytic activity. 3) Based on biochemical properties and sequencing results, the Y15 was named Lactobacillus curvatus Y15. 4) Lactobacillus curvatus Y15 grew quickly at 37 ℃ to reach a level of stable good acid-producing level in 16 h. 5) Lactobacillus curvatus Y15 via gavage had no significant effects on the body signs, secretions, excretions, body weight gain, feed intake, body cavity organs and hematological parameters of mice (P>0.05), could significantly increase the number of colonic goblet cells in colon tissues (P < 0.05), significantly reduced the liver index (P < 0.05), and significantly increased the serum total protein content (P < 0.05). In addition, intraperitoneal injection of Lactobacillus curvatus Y15 into mice did not result in any significant changes of body weight (P>0.05), and no obvious pathological changes in internal organs. In conclusion, Lactobacillus curvatus Y15 isolated from sheep has good probiotic properties, and it do not induce any acute toxicity that passed the in vivo safety test in mice.

Cite this article

XIANG Weiwei , YE Mengjun , HOU Meng , YIN Fangfang , LIU Yawei , WANG Yinlong , PAN Rui , WANG Jinquan . Isolation and Safety Evaluation of a Sheep-Derived Lactobacillus curvatus[J]. Chinese Journal of Animal Nutrition, 2022 , 34(10) : 6790 -6800 . DOI: 10.3969/j.issn.1006-267x.2022.10.071

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