RESEARCH PAPER

Effects of Sodium Butyrate Supplementation during Late Gestation on Growth Performance, Nutrient Apparent Digestibility, Serum Antioxidant and Immune Indices of Hu Sheep Ewes and Growth Performance of Lambs

  • ZHANG Xinyan ,
  • DUAN Chunhui ,
  • YANG Ruochen ,
  • ZHANG Saiwei ,
  • LI Fei ,
  • ZHANG Yingjie
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  • 1. College of Animal Science and Technology, Hebei Agricultural University, Baoding 071000, China;
    2. Animal Husbandry Station of Hebei, Shijiazhuang 050000, China

Received date: 2022-04-15

  Online published: 2022-10-17

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Abstract

The purpose of this experiment was to study the effects of different levels of sodium butyrate supplementation during late gestation on growth performance, nutrient apparent digestibility, serum antioxidant and immune indices of ewes and growth performance of lambs. Sixty female Hu sheep with similar age, body weight and parity at 90 days of gestation were randomly divided into 4 groups, and the basal diet was supplemented with 0 (control group), 2.5 (group A), 5.0 (group B) and 7.5 g/(d·sheep) sodium butyrate (group C), respectively with 3 replicates in each group and 5 sheep in each replicate. The pre-trial period lasted for 7 days, the formal trial period lasted for 90 days, and 15 days before the expected date of delivery, 3 ewes in each group were randomly selected for digestion and metabolism test. During the experiment, feed intake and body weight of ewes were recorded, and blood samples were collected to determine antioxidant and immune indices. Lambing rate, milk yield of ewes and birth weight and 1 month age weight of lambs were calculated after lambing. The results showed as follows: 1) the dry matter intake (DMI) of ewes in group B was higher than that in control group and group A, but had no significant difference (P>0.05). The body weight of ewes in group B was significantly higher than that in control group and group A at prenatal 15 d (P < 0.05). At prenatal 60 d and postpartum 14 d, there was no significant difference in body weight of ewes among all groups (P>0.05). 2) The stillbirth rate of ewes in control group and group A was 7%, and that in group B and C was 0. On postpartum 7 d, milk yield of ewes in groups B and C was significantly higher than that in control group (P < 0.05), but there was no significant difference between groups A and B (P>0.05). There was no significant difference in milk yield between groups on postpartum 1 and 14 d (P>0.05). 3) There were no significant differences in birth weight, 1 months weight and average daily gain of lambs among all groups (P>0.05). 4) The apparent digestibility of dry matter and gross energy of ewes in groups B and C was significantly higher than that in control group and group A (P < 0.05). The apparent digestibility of crude protein in groups A, B and C was significantly higher than that in control group (P < 0.05), but there was no significant difference among the three groups (P>0.05). The apparent digestibility of crude fiber of ewes in group B was significantly higher than that in control group and group A (P < 0.05). There were no significant differences in apparent digestibility of ether extract, Ca and P among groups (P>0.05). 5) At prenatal 60 d, there were no significant differences in the activities of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), malondialdehyde (MDA) content and total antioxidant capacity (T-AOC) in serum between experimental groups (P>0.05). At prenatal 15 d, the serum SOD activity and T-AOC in group B were significantly higher than those in groups A, C and control group (P < 0.05), but there were no significant differences between groups A and C (P>0.05). The serum GSH-Px activity in groups A, B and C was significantly higher than that in control group (P < 0.05), but there was no significant difference among the three groups (P>0.05). On postpartum 14 d, the serum SOD activity in group B was significantly higher than that in group A and control group (P < 0.05), but there was no significant difference between groups B and C (P>0.05). Serum MDA content, GSH-Px activity and T-AOC among all groups did not reach significant levels (P>0.05).6) On prenatal 60 d, there were no significant differences in serum immunoglobulin G (IgG), interleukin-2 (IL-2), interleukin-4 (IL-4) and interleukin-6 (IL-6) contents among experimental groups (P>0.05). At prenatal 15 d, the serum IgG content in group B was significantly higher than that in control group and group C (P < 0.05), but there was no significant difference between groups A and B (P>0.05), presenting significant linear and quadratic changes (P < 0.05). The contents of serum IL-2 and IL-6 in groups B and C were significantly lower than those in control group and group A (P < 0.05), presenting significant linear change (P < 0.05). On postpartum 14 d, the serum IL-6 content in groups B and C was significantly lower than that in control group and group A (P < 0.05). There were no significant differences in serum IgG, IL-2 and IL-4 contents among all groups (P>0.05). In conclusion, dietary sodium butyrate during late gestation can improve feed intake, nutrient apparent digestibility, antioxidant function and immunity of ewes. Under the experimental conditions, the optimal supplemental level of sodium butyrate for Hu sheep ewes is 5.0 g/(d·sheep).

Cite this article

ZHANG Xinyan , DUAN Chunhui , YANG Ruochen , ZHANG Saiwei , LI Fei , ZHANG Yingjie . Effects of Sodium Butyrate Supplementation during Late Gestation on Growth Performance, Nutrient Apparent Digestibility, Serum Antioxidant and Immune Indices of Hu Sheep Ewes and Growth Performance of Lambs[J]. Chinese Journal of Animal Nutrition, 2022 , 34(10) : 6550 -6564 . DOI: 10.3969/j.issn.1006-267x.2022.10.049

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