RESEARCH PAPER

Effects of Modified Attapulgite on Growth Performance, Antioxidant Capacity, Immune Performance and Intestinal Barrier Function of Hy-Line Brown Laying Hens before Laying

  • JIAN Yilong ,
  • LI Lu ,
  • CHEN Yueping ,
  • ZHOU Yanmin ,
  • LIU Qiang ,
  • ZHUANG Su
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  • College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China

Received date: 2022-05-05

  Online published: 2022-11-14

Abstract

This experiment was conducted to investigate the effects of modified attapulgite on growth performance, antioxidant capacity, immune performance and intestinal barrier function of Hy-Line brown laying hens before laying. A total of 960 Hy-Line brown laying chicks of 1-day-old were randomly divided into 4 groups with 6 replicates per group and 40 chicks per replicate. Chickens were fed the basal diet supplemented with 0 (control group), 250, 500 and 1 000 mg/kg modified attapulgite, respectively. The experiment lasted for 90 days. The results showed as follows:1) dietary modified attapulgite could linearly increase the average daily feed intake of laying hens from 1 to 30 days of age (P<0.05). 2) Compared with the control group, the superoxide dismutase (SOD) activity in liver of laying hens at 30 days of age in 1 000 mg/kg modified attapulgite supplemental group was significantly increased (P<0.05); at 60 days of age, the glutathione peroxidase (GSH-Px) activity in serum in the modified attapulgite supplemental groups and in liver in 500 mg/kg modified attapulgite supplemental group was significantly increased (P<0.05), and the SOD activity in serum in 1 000 mg/kg modified attapulgite supplemental group was significantly increased (P<0.05); at 90 days of age, the serum malondialdehyde (MDA) content in 1 000 mg/kg modified attapulgite supplemental group was significantly decreased (P<0.05). 3) Compared with the control group, the immunoglobulin M (IgM) content in ileum of laying hens at 30 days of age in 1 000 mg/kg modified attapulgite supplemental group was significantly increased (P<0.05); at 60 days of age, the secreted immunoglobulin A (sIgA) content in jejunum and the immunoglobulin G (IgG) content in ileum in 250 and 1 000 mg/kg modified attapulgite supplemental groups were significantly increased (P<0.05), the sIgA content in ileum in 1 000 mg/kg modified attapulgite supplemental group was significantly increased (P<0.05), and the IgG content in jejunum in all modified attapulgite supplemental groups was significantly increased (P<0.05); at 90 days of age, the IgM content in ileum in modified attapulgite supplemental groups was significantly increased (P<0.05). 4) Compared with the control group, the villus height to crypt depth ratio (V/C) in jejunum of laying hens at 30 days of age in 1 000 mg/kg modified attapulgite supplemental group was significantly increased (P<0.05), and the villus height and V/C in ileum in 500 mg/kg modified attapulgite supplemental group were significantly increased (P<0.05); at 90 days of age, the villus height and V/C in ileum in 500 and 1 000 mg/kg modified attapulgite supplemental groups were significantly decreased (P<0.05). 5) Compared with the control group, at 90 days of age, the diamine oxidase (DAO) activity and the lipopolysaccharide (LPS) content in serum of laying hens in modified attapulgite supplemental groups were significantly decreased (P<0.05). 6) Compared with the control group, the occludin (OCLN) mRNA relative expression level in jejunum of laying hens at 60 days of age in 500 and 1 000 mg/kg modified attapulgite supplemental groups was significantly increased (P<0.05); at 90 days of age, the zonula occluden-1 (ZO-1) mRNA relative expression level in ileum in 1 000 mg/kg modified attapulgite supplemental group was significantly increased (P<0.05), and the mucin 2 (MUC2) mRNA relative expression level in ileum in 250 mg/kg modified attapulgite supplemental group was significantly increased (P<0.05). In conclusion, dietary modified attapulgite can increase the antioxidant capacity and immune performance, improve the intestinal morphology and enhance the intestinal barrier function of Hy-Line brown laying hens before laying; in this experiment, the optimal supplemental level of modified attapulgite is 1 000 mg/kg.

Cite this article

JIAN Yilong , LI Lu , CHEN Yueping , ZHOU Yanmin , LIU Qiang , ZHUANG Su . Effects of Modified Attapulgite on Growth Performance, Antioxidant Capacity, Immune Performance and Intestinal Barrier Function of Hy-Line Brown Laying Hens before Laying[J]. Chinese Journal of Animal Nutrition, 2022 , 34(11) : 7024 -7037 . DOI: 10.3969/j.issn.1006-267x.2022.11.023

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