Effects of Glucose Oxidase on Laying Performance, Intestinal Tissue Morphology and Cecal Microorganism during Late Laying Period

CHAI Xiaoying; WANG Yan; JIA Xiaoshui; MA Wenfeng; CAO Zhongyang; WANG Zhanbin

doi:10.3969/j.issn.1006-267x.2022.11.024

2022 , Vol. 34 >Issue 11: 7038 - 7048

DOI: https://doi.org/10.3969/j.issn.1006-267x.2022.11.024

RESEARCH PAPER

Effects of Glucose Oxidase on Laying Performance, Intestinal Tissue Morphology and Cecal Microorganism during Late Laying Period

CHAI Xiaoying ,
WANG Yan ,
JIA Xiaoshui ,
MA Wenfeng ,
CAO Zhongyang ,
WANG Zhanbin

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1. College of Animal Science and Technology, Henan University of Science and Technology, Luoyang 471000, China;
2. Henan New Yangshao Biotechnology Co., Ltd., Sanmenxia 472400, China

Received date: 2022-04-19

Online published: 2022-11-14

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Abstract

This experiment was conducted to explore the effects of glucose oxidase (GOD) on laying performance and intestinal health in laying hens in the late laying period. Two hundred and seventy 70-week-old Hy-Line brown laying hens with similar egg-laying rate were selected and randomly divided into 3 groups with 6 replicates in each group and 15 chickens in each replicate, in which the control group was fed a basal diet, and the other two groups added 150 and 300 mg/kg GOD with activity of 1 000 U/g into the basal diet. The pre-test period lasted for 7 days, and the test period lasted for 50 days. The results showed that the egg-laying rate of the 150 mg/kg GOD group and the 300 mg/kg GOD group was significantly higher than that of the control group (P<0.05); the average daily egg weight of the 300 mg/kg GOD group was significantly higher than that of the control group and the 150 mg/kg GOD group (P<0.05), and there was no significant difference between the 150 mg/kg GOD group and the control group (P>0.05). The feed to egg ratio of the 300 mg/kg GOD group was significantly lower than that of the control group (P<0.05), and there was no significant difference between the 150 mg/kg GOD group and the control group (P>0.05). There were no significant differences between the two experimental groups and the control group in terms of average daily feed intake and shell breaking rate (P>0.05). 2) Compared with the control group, the addition of 150 and 300 mg/kg GOD in the diet could significantly increase the villus height of the duodenum, jejunum and ileum (P<0.05); the addition of 300 mg/kg GOD in the diet could significantly increase the villus height/crypto depth of the duodenum (P<0.05). 3) The addition of 300 mg/kg GOD in the diet had no significant effect on the Alpha diversity of microbial community. At the phylum level, the relative abundances of Spirochaetes and Elusimicrobia were extremely significantly increased (P<0.01), and the relative abundances of Cyanobacteria and Verrucomicrobia were significantly or extremely significantly decreased by adding 300 mg/kg GOD in the diet (P<0.05 or P<0.01). At the genus level, the relative abundances of Bacteroides, Ruminococcaceae_unclassified and Ruminococcaceae_NK4A214_group were significantly or extremely significantly increased (P<0.05 or P<0.01), and the relative abundance of Prevotellaceae_UCG-001 was significantly decreased by adding 300 mg/kg GOD in the diet (P<0.05). It can be seen from the above that adding GOD in the diet can improve the laying performance of laying hens in the late laying period, improve the intestinal tissue morphology, and has no significant effect on the cecal microbial diversity, but the structural composition of the cecum microbial community is optimized.

Key words： glucose oxidase; laying hens; late laying period; laying performance; intestinal tissue morphology; cecal microorganism; 16S rDNA

Cite this article

CHAI Xiaoying , WANG Yan , JIA Xiaoshui , MA Wenfeng , CAO Zhongyang , WANG Zhanbin . Effects of Glucose Oxidase on Laying Performance, Intestinal Tissue Morphology and Cecal Microorganism during Late Laying Period[J]. Chinese Journal of Animal Nutrition, 2022 , 34(11) : 7038 -7048 . DOI: 10.3969/j.issn.1006-267x.2022.11.024

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