RESEARCH PAPER

Effects of Mulberry Leaf Extract on Abdominal Fat Deposition and Related Lipid Metabolism Indexes in Broilers

  • HUANG Tailai ,
  • JIN Rui ,
  • WEN Jianchong ,
  • TAN Hua ,
  • LEI Huaying ,
  • CAO Manhu
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  • 1. College of Animal Science and Technology, Hunan Agricultural University, Changsha 410128, China;
    2. Hunan Jinhan Pharmaceutical Co., Ltd., Changsha 410300, China;
    3. Mayang Blue Phoenix Agricultural Development Co., Ltd., Huaihua 419400, China

Received date: 2022-04-06

  Online published: 2022-11-14

Abstract

This experiment was conducted to investigate the effects of mulberry leaf extract on abdominal fat deposition and related metabolic indexes of broilers. A total of 192 one-day-old healthy AA broilers with similar body size were randomly divided into 4 groups with 6 replicates per group and 8 broilers per replicate. Broilers in control group were fed a basal diet, and those in experimental groups were fed the basal diet supplemented with 400, 800 and 1 200 mg/kg mulberry leaf extract, respectively. The experiment lasted for 56 days and was divided into two stages:1 to 21 days of age (early stage) and 22 to 56 days of age (late stage). The growth performance, serum and liver lipid metabolism related indexes of broilers were detected. The results showed as follows:compared with control group, 1) dietary mulberry leaf extract had no significant effects on average daily feed intake (ADFI), average daily gain (ADG) and feed/gain (F/G) of broilers during the whole period (P<0.05); 2) adding mulberry leaf extract significantly decreased the abdominal fat deposition of broilers in two experimental stages (P<0.05); 3) adding 800 and 1 200 mg/kg mulberry leaf extract significantly reduced serum total cholesterol (TC) content in early and late stage, respectively; TC content in liver was significantly decreased (P<0.05); 4) adding mulberry leaf extract significantly increased the mRNA relative expression level of AMP-activated protein kinase α (AMPKα) in liver (P<0.05), adding 800 and 1 200 mg/kg mulberry leaf extract significantly decreased the mRNA relative expression levels of sterol regulatory element binding protein-1c (SREBP-1c), acetyl-CoA carboxylase (ACC) and fatty acid synthetase (FAS) in liver (P<0.05). In conclusion, adding 400, 800 and 1 200 mg/kg mulberry leaf extract can reduce abdominal fat deposition of AA broilers. The mechanism of lipid lowering effect of mulberry leaf extract is as follows:mulberry leaf extract significantly increases the expression of AMPKα gene in liver, and significantly lower the expression of SREBP-1c, FAS, ACC genes in liver which is related to fat metabolism. By reducing fat synthesis, the content of TC in liver and serum is reduced, and its mechanism may be associated with AMPK/SREBP-1c/ACC pathway.

Cite this article

HUANG Tailai , JIN Rui , WEN Jianchong , TAN Hua , LEI Huaying , CAO Manhu . Effects of Mulberry Leaf Extract on Abdominal Fat Deposition and Related Lipid Metabolism Indexes in Broilers[J]. Chinese Journal of Animal Nutrition, 2022 , 34(11) : 7072 -7081 . DOI: 10.3969/j.issn.1006-267x.2022.11.027

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