RESEARCH PAPER

Effects of Bacillus subtilis on Growth Performance and Intestinal Health of Broilers Attacked by Coccidia and Clostridium perfringens

  • DENG Peijie ,
  • SONG Zehe ,
  • LUO Guosheng ,
  • LIU Yali ,
  • JIANG Xiaofeng ,
  • LIU Zikui ,
  • HE Xi
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  • 1. College of Animal Medicine, Hunan Agricultural University, Changsha 410128, China;
    2. Hunan Poultry Safety Production Engineering Technology Research Center, College of Animal Science and Technology, Hunan Agricultural University, Changsha 410128, China;
    3. Hunan Perfly Biotech Co., Ltd., Changsha 410128, China

Received date: 2022-04-19

  Online published: 2022-11-14

Abstract

This experiment was conducted to investigate the effects of Bacillus subtilis on growth performance and intestinal health of broilers attacked by coccidia and Clostridium perfringens. A total of 720 one-day-old Arbor Acres male broilers were selected and randomly divided into 8 groups with 6 replicates in each group and 15 birds in each replicate. The experiment was set up in a 2×4 factorial design with four treatments on the basis of attack and no attack, which feeding the basal diet, basal diet+300 mg/kg Bacillus subtilis, basal diet+500 mg/kg Bacillus subtilis and basal diet+12 mg/kg enramycin, respectively. Broilers in the attack groups were attacked by coccidia at 14 days of age and attacked by Clostridium perfringens at 19 to 21 days of age. Broilers were caged and fed and watered ad libitum for a trial period of 42 days. The results were as follows:1) the attack significantly increased the feed to gain ratio of broilers during 22 to 42 days of age (P<0.05), significantly increased the serum diamine oxidase activity at 28 and 35 days of age and serum lipopolysaccharide content at 35 days of age of broilers (P<0.05), significantly decreased the villus height and villus height/crypt depth in jejunum and ileum at 28 and 35 days of age of broilers (P<0.05), significantly increased the crypt depth in jejunum and ileum at 28 and 35 days of age of broilers (P<0.05), significantly increased the mRNA relative expression levels of tumor necrosis factor-α (TNF-α), interferon-γ (IFN-γ), nuclear transcription factor-κB (NF-κB) and interleukin-6 (IL-6) in jejunum at 28 and 35 days of age of broilers (P<0.05), and significantly increased the mRNA relative expression level of interleukin-12 (IL-12) in jejunum at 28 days of age of broilers (P<0.05). 2) Compared with the basal diet and addition of 500 mg/kg Bacillus subtilis, the addition of 300 mg/kg Bacillus subtilis significantly increased the average daily gain and final body weight of broilers during 1 to 21 days of age and 22 to 42 days of age (P<0.05), and significantly decreased the feed to gain ratio of broilers during 22 to 42 days of age (P<0.05). Compared with the basal diet, the addition of 500 mg/kg Bacillus subtilis significantly decreased the serum lipopolysaccharide content at 28 days of age of broilers (P<0.05), the addition of 300 mg/kg Bacillus subtilis significantly increased the ileum villi height at 28 and 35 days of age of broilers (P<0.05), and the addition of 12 mg/kg enramycin and 300 mg/kg Bacillus subtilis significantly increased the mRNA expression levels of TNF-α, NF-κB and IL-6 in jejunum at 35 days of age of broilers (P<0.05). 3) There were interactive effects on of attack and diet on the feed to gain ratio of broilers during 22 to 42 days of age, the intestinal morphology (except jejunal crypt depth) at 35 days of age, the mRNA relative expression levels of NF-κB and IL-6 in jejunum at 28 days of age, and the mRNA relative expression levels of TNF-α, NF-κB and IL-6 in jejunum at 35 days of age (P<0.05). In conclusion, the Bacillus subtilis can alleviate the negative impact of broilers attacked by coccidia and Clostridium perfringens, improve the growth performance and intestinal health to a certain extent, and the addition of 300 mg/kg Bacillus subtilis is better than the addition of 500 mg/kg.

Cite this article

DENG Peijie , SONG Zehe , LUO Guosheng , LIU Yali , JIANG Xiaofeng , LIU Zikui , HE Xi . Effects of Bacillus subtilis on Growth Performance and Intestinal Health of Broilers Attacked by Coccidia and Clostridium perfringens[J]. Chinese Journal of Animal Nutrition, 2022 , 34(11) : 7082 -7093 . DOI: 10.3969/j.issn.1006-267x.2022.11.028

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