RESEARCH PAPER

Effects of Compound Additives on Growth Performance, Serum Immune and Antioxidant Function and Intestine Health of Ningdu Yellow Chickens

  • SONG Qiongli ,
  • SONG Wenjing ,
  • ZOU Zhiheng ,
  • CHEN Xiaolian ,
  • WEI Qipeng ,
  • LIU Linxiu ,
  • XIONG Pingwen ,
  • YANG Ting ,
  • RAO Yuling
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  • Institute of Animal Husbandry and Veterinary Science, Jiangxi Academy of Agricultural Sciences, Nanchang 330200, China

Received date: 2022-05-13

  Online published: 2022-11-14

Abstract

The aim of this study was to evaluate the effects of compound additives with sodium butyrate, radix astragali extract and compound probiotics as an alternative to antibiotic on growth performance, serum immune and antioxidant function and intestine health of Ningdu yellow chickens. A total of 648 healthy Ningdu yellow female chicks of 1-day-old with similar body weight were randomly divided into 6 groups with 6 replicates per group and 18 chicks per replicate. Chickens in group Ⅰ (control group) were fed a basal diet, those in group Ⅱ (antibiotic group) were fed the basal diet supplemented with 150 mg/kg aureomycin, those in group Ⅲ were fed the basal diet supplemented with 500 mg/kg sodium butyrate and 500 mg/kg radix astragali extract, those in group Ⅳ were fed the basal diet supplemented with 500 mg/kg sodium butyrate and 200 mg/kg compound probiotics, those in group Ⅴ were fed the basal diet supplemented with 500 mg/kg radix astragali extract and 200 mg/kg compound probiotics, and those in group Ⅵ were fed the basal diet supplemented with 500 mg/kg sodium butyrate, 500 mg/kg radix astragali extract and 200 mg/kg compound probiotics, respectively. The experiment lasted for 56 days. The results showed as follows:1) compared with group Ⅰ, the body weight at 56 days of age and average daily gain (ADG) of Ningdu yellow chickens in groups Ⅳ, Ⅴ and Ⅵ were significantly increased (P<0.05), and the ratio of feed to gain (F/G) in groups Ⅲ, Ⅳ, Ⅴ and Ⅵ was significantly decreased (P<0.05). Compared with group Ⅱ, the F/G in group Ⅵ was significantly decreased (P<0.05). 2) Compared with group Ⅱ, the contents of immunoglobulin M (IgM), the activities of glutathione peroxidase (GSH-Px) and superoxide dismutase (SOD) and the total antioxidant capacity (T-AOC) in serum of Ningdu yellow chickens in groups Ⅲ, Ⅳ, Ⅴ and Ⅵ were significantly increased (P<0.05), while the contents of interleukin-2 (IL-2), tumor necrosis factor-α (TNF-α) and interferon-γ (IFN-γ) in serum were significantly decreased (P<0.05); the contents of immunoglobulin G (IgG), immunoglobulin A (IgA) and interleukin-4 (IL-4) in serum in groups Ⅳ, Ⅴ and Ⅵ were significantly increased (P<0.05), while the malondialdehyde (MDA) content in serum was significantly decreased (P<0.05). 3) Compared with group Ⅰ, the ratio of villus height to crypt depth (V/C) in duodenum of Ningdu yellow chickens in groups Ⅲ and Ⅳ was significantly increased (P<0.05), the crypt depth in jejunum in groups Ⅳ and Ⅴ was significantly decreased (P<0.05), and the V/C in jejunum in groups Ⅲ, Ⅳ and Ⅴ was significantly increased (P<0.05), but there was no significant difference compared with group Ⅱ (P>0.05). 4) Compared with group Ⅱ, the Shannon index in cecal microflora of Ningdu yellow chickens in groups Ⅲ and Ⅵ was significantly increased (P<0.05); the Firmicutes relative abundance in groups Ⅲ and Ⅴ was significantly increased (P<0.05), and the Bacteroidetes relative abundance was significantly decreased (P<0.05); the Bacteroides relative abundance in groups Ⅲ, Ⅴ and Ⅵ was significantly decreased (P<0.05). In conclusion, dietary sodium butyrate, radix astragali extract and compound probiotics in combination have the potential to replace antibiotics by improving the growth performance, serum immune and antioxidant function and morphological structure of small intestine, maintaining the intestinal microbe homeostasis, and promoting the intestine health of Ningdu yellow chickens.

Cite this article

SONG Qiongli , SONG Wenjing , ZOU Zhiheng , CHEN Xiaolian , WEI Qipeng , LIU Linxiu , XIONG Pingwen , YANG Ting , RAO Yuling . Effects of Compound Additives on Growth Performance, Serum Immune and Antioxidant Function and Intestine Health of Ningdu Yellow Chickens[J]. Chinese Journal of Animal Nutrition, 2022 , 34(11) : 7120 -7132 . DOI: 10.3969/j.issn.1006-267x.2022.11.031

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