RESEARCH PAPER

Effects of Plant Essential Oil Eutectic on Growth Performance, Intestinal Morphology, Digestive Enzyme Activity and Antioxidant Capacity of Meat Ducks

  • LI Lixuan ,
  • ZHANG Keying ,
  • DING Xuemei ,
  • BAI Shiping ,
  • WANG Jianping ,
  • LYU Li ,
  • XUAN Yue ,
  • ZENG Qiufeng
Expand
  • Key Laboratory for Animal Disease-Resistance Nutrition of Ministry of Education, Ministry of Agriculture and Rural Affaires, Sichuan Province, Institute of Animal Nutrition, Sichuan Agricultural University, Chengdu 611130, China

Received date: 2022-04-28

  Online published: 2022-11-14

Abstract

This study aimed to evaluate the effects of plant essential oil eutectic on growth performance and intestinal health of meat ducks by feeding experiment, and to explore the optimal addition level of plant essential oil eutectic in meat duck diet by linear and quadratic curve analysis. A total of 480 cherry valley ducks, aged 1 day old with similar body weight were randomly divided into 5 groups (6 replicates per group,16 ducks per replicate). The ducks in control group and 4 experimental groups were fed plant essential oil eutectic of 0,15,30,60 and 120 mg/kg,respectively. The plant essential oil eutectic was a 1:1 eutectic of thymol and carvacrol, and its effective content is 50%. The experiment lasted for 35 days. The experiment was divided into the early stage (1 to 14 days of age) and the later stage (15 to 35 days of age). The results showed as follows:1)there were no significant differences in the growth performance and the contents of total bacteria, Salmonella and Escherichia coli in caecal chyme among all groups (P>0.05), but with increasing the dietary plant essential oil eutectic level, the mortality rate was linearly decreased in the early stage(P<0.05). 2) Compared with the control group, the jejunum crypt depth of meat ducks at 14 days of age was significantly increased by adding different supplemental levels of plant essential oil eutectic (P<0.05), and the ratio of villus height to crypt depth at 14 days of age and jejunum crypt depth at 35 days of age were significantly decreased with the increase of the plant essential oil eutectic level(P<0.05), dietary supplemented with 60 and 120 mg/kg plant essential oil eutectic was significantly increased the villus height (P<0.05), but the villus height was significantly decreased at 35 days of age (P<0.05), and the supplementation of 30 mg/kg plant essential oil eutectic significantly increased the ratio of villus height to crypt depth(P<0.05). 3) Compared with the control group, adding 120 mg/kg plant essential oil eutectic significantly increased jejunum amylase activity at 35 days of age (P<0.05). 4) Compared with the control group, dietary supplemented with 60 and 120 mg/kg plant essential oil eutectic significantly increased the content of malonaldehyde (MDA) in ileum mucosa at 35 days of age (P<0.05). Myeloperoxidase (MPO) activity in 30 and 120 mg/kg plant essential oil eutectic groups was significantly increased (P<0.05). These results suggest that the effects of plant essential oils are dose-dependent, it is recommended to add 60 mg/kg essential oil eutectic for meat ducks according to intestinal morphology and considering growth performance and digestive enzyme activities.

Cite this article

LI Lixuan , ZHANG Keying , DING Xuemei , BAI Shiping , WANG Jianping , LYU Li , XUAN Yue , ZENG Qiufeng . Effects of Plant Essential Oil Eutectic on Growth Performance, Intestinal Morphology, Digestive Enzyme Activity and Antioxidant Capacity of Meat Ducks[J]. Chinese Journal of Animal Nutrition, 2022 , 34(11) : 7145 -7157 . DOI: 10.3969/j.issn.1006-267x.2022.11.033

References

[1] 杨永胜.植物精油的主要提取技术、应用及研究进展[J].当代化工研究, 2021(4):153-154. YANG Y S.Main extraction technology, application and research progress of plant essential oil[J].Modern Chemical Research, 2021(4):153-154.(in Chinese)
[2] 薛萍.植物精油在畜禽生产中的应用研究进展[J].福建畜牧兽医, 2020, 42(6):33-36. XUE P.Research progress in the application of plant essential oil in livestock and poultry production[J].Journal of Animal Husbandry and Veterinary Medicine Fujian, 2020, 42(6):33-36.(in Chinese)
[3] 牛小杰, 孙鲁阳.植物精油化学成分的研究进展[J].生物化工, 2021, 7(5):160-162. NIU X J, SUN L Y.Research progress on chemical constituents of plant essential oil[J].Biological Chemical Engineering, 2021, 7(5):160-162.(in Chinese)
[4] KARPIÑSKI T M.Essential oils of Lamiaceae family plants as antifungals[J].Biomolecules, 2020, 10(1):103.
[5] 唐镇海, 袁建敏.植物精油替抗的研究进展及机理[J].饲料工业, 2021, 42(2):18-23. TANG Z H, YUAN J M.Research progress and mechanism of plant essential oil replacing antibiotic growth promoter[J].Feed Industry, 2021, 42(2):18-23.(in Chinese)
[6] LOMBREA A, ANTAL D, ARDELEAN F, et al.A recent insight regarding the phytochemistry and bioactivity of Origanum vulgare L.essential oil[J].International Journal of Molecular Sciences, 2020, 21(24):9653.
[7] 闫世雄, 郝亭亭, 张芯燕, 等.香芹酚和百里香酚对畜禽肠道健康的调控[J].饲料研究, 2022, 45(3):129-133. YAN S X, HAO T T, ZHANG X Y, et al.Regulation mechanism of carvacrol and thymol on intestinal health of livestock and poultry[J].Feed Research, 2022, 45(3):129-133.(in Chinese)
[8] 刘娇, 常文环, AHMED P S, 等.植物精油对大肠杆菌攻毒肉鸭生长性能、血清生化指标和免疫功能的影响[J].动物营养学报, 2020, 32(8):3670-3680. LIU J, CHANG W H, AHMED P S, et al.Effects of plant essential oil on growth performance, serum biochemical indexes and immune function of ducks challenged by Escherichia coli[J].Chinese Journal of Animal Nutrition, 2020, 32(8):3670-3680.(in Chinese)
[9] 吴昊, 张兰, 王贵敏, 等.单一或复合植物精油添加对麻黄肉鸡生长性能、肉品质和抗氧化能力的影响[J].动物营养学报, 2022, 34(5):2970-2979. WU H, ZHANG L, WANG G M, et al. Effects of single or compound plant essential oils on growth performance, meat quality and antioxidant capacity of Mahuang broilers[J].Chinese Journal of Animal Nutrition, 2022, 34(5):2970-2979.(in Chinese)
[10] SU G Q, WANG L, ZHOU X W, et al.Effects of essential oil on growth performance, digestibility, immunity, and intestinal health in broilers[J].Poultry Science, 2021, 100(8):101242.
[11] XUE F G, SHI L, LI Y L, et al.Effects of replacing dietary aureomycin with a combination of plant essential oils on production performance and gastrointestinal health of broilers[J].Poultry Science, 2020, 99(9):4521-4529.  
[12] WINDISCH W, SCHEDLE K, PLITZNER C, et al.Use of phytogenic products as feed additives for swine and poultry[J].Journal of Animal Science, 2008, 86(14 Suppl.):E140-E148.
[13] 吴帆, 倪伟超, 李彪, 等.植物精油的提取方法及其功能特性研究进展[J].中国野生植物资源, 2016, 35(5):47-51. WU F, NI W C, LI B, et al.Research progress on extraction method and functional property of plant volatile oils[J].Chinese Wild Plant Resources, 2016, 35(5):47-51.(in Chinese)
[14] 聂振昌, 程宗佳, 李辉, 等.植物精油的微胶囊化及在养猪生产中的应用研究[J].饲料博览, 2021(5):10-15. NIE Z C, CHENG Z J, LI H, et al.Microencapsulation of plant essential oil and its application reserch in pig production[J].Feed Review, 2021(5):10-15.(in Chinese)
[15] VALDIVIESO-UGARTE M, GOMEZ-LLORENTE C, PLAZA-DÍAZ J, et al.Antimicrobial, antioxidant, and immunomodulatory properties of essential oils:a systematic review[J].Nutrients, 2019, 11(11):2786.
[16] ANTONIOLI G, FONTANELLA G, ECHEVERRIGARAY S, et al.Poly (lactic acid) nanocapsules containing lemongrass essential oil for postharvest decay control:in vitro and in vivo evaluation against phytopathogenic fungi[J].Food Chemistry, 2020, 326:126997.
[17] FAWAL G E, ABU-SERIE M M.Bioactive properties of nanofibers based on poly (vinylidene fluoride) loaded with oregano essential oil:fabrication, characterization and biological evaluation[J].Journal of Drug Delivery Science and Technology, 2022(69):103133.
[18] LI L, ZHANG W H, PENG J, et al.A novel shell material-highland barley starch for microencapsulation of cinnamon essential oil with different preparation methods[J].Materials, 2020, 13(5):1192.
[19] TOMAZELLI JÚNIOR O, KUHN F, PADILHA P J M, et al.Microencapsulation of essential thyme oil by spray drying and its antimicrobial evaluation against Vibrio alginolyticus and Vibrio parahaemolyticus[J].Brazilian Journal of Biology, 2018, 78(2):311-317.
[20] DUGGIRALA N K, PERRY M L, ALMARSSON Ö, et al.Pharmaceutical cocrystals:along the path to improved medicines[J].Chemical Communications (Cambridge England), 2016, 52(4):640-655.  
[21] 王礼丽, 高紫尧, 刘书妤.药物共晶的研究新进展[J].中国医药工业杂志, 2021, 52(7):881-890. WANG L L, GAO Z Y, LIU S Y.New research progress of pharmaceutical cocrystals[J].Chinese Journal of Pharmaceuticals, 2021, 52(7):881-890.(in Chinese)
[22] LIAO H, WU X P, ZHANG K Y, et al.The effect of citric acid acidification of drinking water on growth performance, cecal pH, and cecal microflora of meat duck[J].Livestock Science, 2018, 209:54-59.
[23] PARK J H, KANG S N, SHIN D, et al.Antioxidant enzyme activity and meat quality of meat type ducks fed with dried oregano (Origanum vulgare L.) powder[J].Asian Australasian Journal of Animal Sciences, 2015, 28(1):79-85.
[24] ABOUELEZZ K, ABOU-HADIED M, YUAN J, et al.Nutritional impacts of dietary oregano and Enviva essential oils on the performance, gut microbiota and blood biochemicals of growing ducks[J].Animal an International Journal of Animal Bioscience, 2019, 13(10):2216-2222.  
[25] RUAN D, FAN Q L, FOUAD A M, et al.Effects of dietary oregano essential oil supplementation on growth performance, intestinal antioxidative capacity, immunity, and intestinal microbiota in yellow-feathered chickens[J].Journal of Animal Science, 2021, 99(2):skab033.
[26] YOUSSEF I M I, MÄNNER K, ZENTEK J.Effect of essential oils or saponins alone or in combination on productive performance, intestinal morphology and digestive enzymes activity of broiler chickens[J].Journal of Animal Physiology and Animal Nutrition, 2021, 105(1):99-107.  
[27] 张晓燕, 梁光哲, 王铎, 等.植物精油对广西灵山土鸡生长性能、养分消化率和血清抗体指标的影响[J].中国畜牧杂志, 2022, 58(2):198-202. ZHANG X Y, LIANG G Z, WANG D, et al.Effects of essential oil on growth performance, nutrient digestibility and serum antibody indices of Guangxi Lingshan chicken[J].Chinese Journal of Animal Science, 2022, 58(2):198-202.(in Chinese)
[28] 江小帆, 吴涛, 魏玉明, 等.饲粮中添加牛至精油对芦花鸡生长性能、屠宰性能、器官指数和肠道形态的影响[J].浙江农业学报, 2022, 34(1):41-49. JIANG X F, WU T, WEI Y M, et al.Effects of dietary oregano essential oil on growth performance, slaughter performance, organ indexes and intestinal morphology of Luhua chickens[J].Acta Agriculturae Zhejiangensis, 2022, 34(1):41-49.(in Chinese)
[29] 李文菁, 魏凯敏, 张少秋, 等.核桃青皮及其提取物对黄羽肉仔鸡肠道形态、黏膜抗氧化性能及微生物多样性的影响[J].中国畜牧兽医, 2021, 48(6):2056-2065. LI W J, WEI K M, ZHANG S Q, et al.Effects of walnut green husk and its extract on intestinal morphology, mucosal antioxidant activity and microbial diversity of yellow-feather broilers[J].China Animal Husbandry & Veterinary Medicine, 2021, 48(6):2056-2065.(in Chinese)
[30] 徐静, 张子儒, 王德贺, 等.饮水中添加大蒜精油对蛋鸡生长性能、肠道组织形态及盲肠菌群的影响[J].动物营养学报, 2021, 33(1):308-316. XU J, ZHANG Z R, WANG D H, et al.Effects of adding garlic essential oil into drinking water on growth performance, intestinal tissue morphology and cecum microbial flora of layer hens[J].Chinese Journal of Animal Nutrition, 2021, 33(1):308-316.(in Chinese)
[31] 梁万礼, 王永昌, 王四维.消化酶、消化吸收与饲料加工[J].粮食与饲料工业, 2021(1):36-40, 43. LIANG W L, WANG Y C, WANG S W.Digestive enzymes, digestion and absorption and feed processing[J].Cereal & Feed Industry, 2021(1):36-40, 43.(in Chinese)
[32] 闻爱友, 胡洪, 吴学壮, 等.复合植物精油对肉鸡生产性能、消化酶活性及糖脂代谢的影响[J].安徽科技学院学报, 2019, 33(2):13-19. WEN A Y, HU H, WU X Z, et al.Effects of plant essential oil compound on performance, digestive enzyme activity and glucose and lipid metabolism of broilers[J].Journal of Anhui Science and Technology University, 2019, 33(2):13-19.(in Chinese)
[33] 李红英, 黄小红, 黄恩福, 等.植物精油对山麻鸭产蛋性能、蛋品质和消化酶活性的影响[J].饲料研究, 2020, 43(7):44-47. LI H Y, HUANG X H, HUANG E F, et al.Effect of essential oils on performance, egg quality and digestive enzyme activity of Shan partridge duck[J].Feed Research, 2020, 43(7):44-47.(in Chinese)
[34] ALAGAWANY M, EL-SAADONY M T, ELNESR S S, et al.Use of lemongrass essential oil as a feed additive in quail's nutrition:its effect on growth, carcass, blood biochemistry, antioxidant and immunological indices, digestive enzymes and intestinal microbiota[J].Poultry Science, 2021, 100(6):101172.
[35] 丁雪瑶, 刘思伽, 方心灵, 等.植物提取物在畜禽无抗饲料中的应用[J].广东畜牧兽医科技, 2021, 46(3):13-17. DING X Y, LIU S J, FANG X L, et al.Application of plant extracts in non-antibiotic feed for livestock and poultry[J].Guangdong Journal of Animal and Veterinary Science, 2021, 46(3):13-17.(in Chinese)
[36] 温朋飞, 彭艳.植物精油抗氧化作用机制研究进展[J].饲料工业, 2017, 38(2):40-45. WEN P F, PENG Y.Research advances on antioxidant mechanism of plant essential oil[J].Feed Industry, 2017(2):40-45.(in Chinese)
[37] 张成, 赵菲, 李煬, 等.EGCG对急性热应激肉鸡抗氧化能力的影响[J].中国农业大学学报, 2021, 26(10):139-148. ZHANG C, ZHAO F, LI Y, et al.Effects of EGCG on antioxidant capacity of acute heat-stressed broilers[J].Journal of China Agricultural University, 2021, 26(10):139-148.(in Chinese)
[38] 李拥军.丙戊酸钠对癫痫患者甲状腺功能及SOD、MPO、MDA水平的影响[J].现代医学与健康研究电子杂志, 2021, 5(1):46-48. LI Y J.Effects of valproate on thyroid function and levels of SOD, MPO and MDA in epileptic patients[J].Modern Medicine and Health Research Electronic Journal, 2021, 5(1):4648.(in Chinese)
[39] 薄磊, 单既刚, 赵志文.心血管疾病中髓过氧化物酶的研究进展[J].检验医学, 2017, 32(8):738-743. BO L, SHAN J G, ZHAO Z W.Research progress of myeloperoxidase in cardiovascular diseases[J].Laboratory Medicine, 2017, 32(8):738-743.(in Chinese)
[40] ZHANG L Y, PENG Q Y, LIU Y R, et al.Effects of oregano essential oil as an antibiotic growth promoter alternative on growth performance, antioxidant status, and intestinal health of broilers[J].Poultry Science, 2021, 100(7):101163.
[41] 马伟斌, 高宪儒.日粮中添加植物精油对肉仔鸡生长性能、免疫及抗氧化功能的影响[J].饲料研究, 2021, 44(5):47-50. MA W B, GAO X R.Effect of adding plant essential oils in diets on growth performance, immunity and antioxidant functions of broilers[J].Feed Research, 2021, 44(5):47-50.(in Chinese)
[42] 朱晓磊, 陈宏.百里香精油对麻花鸡生长性能、养分利用率、血清生化指标和肠道菌群的影响[J].中国家禽, 2013, 35(21):21-26. ZHU X L, CHEN H.Effects of dietary different levels of thyme essential oils on growth performance, nutrients utilization, serum biochemical parameters and intestinal flora of broilers[J].China Poultry, 2013, 35(21):21-26.(in Chinese)
[43] 王改琴, 邬本成, 王宇霄, 等.不同植物精油体外抑菌效果的研究[J].国外畜牧学(猪与禽), 2014, 34(4):50-52. WANG G Q, WU B C, WANG Y X, et al.In vitro inhibition efficacy of different plant essential oils[J].Animal Science Abroad (Pigs and Poultry), 2014, 34(4):50-52.(in Chinese)
[44] 张嘉琦, 张会艳, 赵青余, 等.植物精油对畜禽肠道健康、免疫调节和肉品质的研究进展[J].动物营养学报, 2021, 33(5):2439-2451. ZHANG J Q, ZHANG H Y, ZHAO Q Y, et al.Research progress of plant essential oil on intestinal health, immune regulation and meat quality of livestock and poultry[J].Chinese Journal of Animal Nutrition, 2021, 33(5):2439-2451.(in Chinese)
[45] 张艳雯, 玉发杨, 何永权, 等.不同营养水平日粮中添加精油对肉鸡生长性能、组织器官重量、盲肠菌群及血液生化指标的影响[J].中国饲料, 2018(18):18-22. ZHANG Y W, YU F Y, HE Y Q, et al.Effects of supplementation of essential oil in different nutrient level diets on growth performance, relative organ weight, cecal microflora and blood biochemistry indices of broilers[J].China Feed, 2018(18):18-22.(in Chinese)
[46] CETIN E, YIBAR A, YESILBAG D, et al.The effect of volatile oil mixtures on the performance and ilio-caecal microflora of broiler chickens[J].British Poultry Science, 2016, 57(6):780-787.  
[47] CASTAÑEDA-CORREA A, CORRAL-LUNA A, HUME M E, et al.Effects of thymol and carvacrol, alone or in combination, on fermentation and microbial diversity during in vitro culture of bovine rumen microbes[J].Journal of Environmental Science and Health Part B Pesticides Food Contaminants and Agricultural Wastes, 2019, 54(3):170-175.
Outlines

/