RESEARCH PAPER

Evaluation on Silage Quality and Feeding Value of Pineapple Residue by EM Bacteria

  • WU Zhengmin ,
  • QIU Xiaoyuan ,
  • WU Pengxin ,
  • XU Yuewen ,
  • WANG Jialin ,
  • YANG Kang ,
  • YIN Fuquan
Expand
  • College of Coastal Agricultural Sciences, Guangdong Ocean University, Zhanjiang 524088, China

Received date: 2022-05-11

  Online published: 2022-11-14

Abstract

This experiment was conducted to study the effects of different supplemental doses of EM bacteria on the quality of pineapple pomace silage and its feeding value evaluation, in order to provide reference for the development of pineapple residue resources. The single factor experiment design was used. The amount of EM bacteria additive was 0, 24, 48 and 96 mL/kg, respectively. The control group and groups Ⅰ, Ⅱ, and Ⅲ were mixed with pineapple residue and silaged for 30 days. The related indexes were determined and analyzed, and the in vitro gas production experiment was carried out. The results showed as follows:1) in the silage experiment, compared with control group, the ammonia nitrogen/total nitrogen (NH3-N/TN) was significantly decreased (P<0.05), the contents of crude protein (CP) and soluble carbon water (WSC) in group Ⅱ were significantly increased (P<0.05). WSC content in group Ⅲ was significantly decreased (P<0.05). With the increase of EM bacteria additive amount, the ash (Ash) content showed an upward trend (P=0.077). 2) The results of in vitro gas production experiment showed that the gas production (GP) and ammonia nitrogen (NH3-N) content in group Ⅲ were significantly higher than those in groups Ⅰ and Ⅱ at 48 h (P<0.05). Compared with the control group, the CP degradation rates in groups Ⅱ and Ⅲ were significantly increased after 48 h of fermentation (P<0.05). In summary, the quality and feeding value of pineapple residue silage can be improved by adding EM bacteria. Under the conditions of this experiment, the optimal addition level of EM bacteria is 48 mL/kg.

Cite this article

WU Zhengmin , QIU Xiaoyuan , WU Pengxin , XU Yuewen , WANG Jialin , YANG Kang , YIN Fuquan . Evaluation on Silage Quality and Feeding Value of Pineapple Residue by EM Bacteria[J]. Chinese Journal of Animal Nutrition, 2022 , 34(11) : 7307 -7316 . DOI: 10.3969/j.issn.1006-267x.2022.11.048

References

[1] 谭慎宋.徐闻县菠萝面积与产量遥感估测关键技术研究[D].硕士学位论文.湛江:广东海洋大学, 2021. TAN S S.Study on key techniques of remote sensing estimation of pineapple area and yield in Xuwen county[D].Master's Thesis.Zhenjiang:Guangdong Ocean University, 2021.(in Chinese)
[2] 王晓敏, 刘培剑.菠萝渣在动物生产中的应用研究进展[J].广东饲料, 2016, 25(1):41-42. WANG X M, LIU P J.Research progress on application of pineapple residue in animal production[J].Guangdong Feed, 2016, 25(1):41-42.(in Chinese)
[3] GOWDA N K S, VALLESHA N C, AWACHAT V B, et al.Study on evaluation of silage from pineapple (Ananas comosus) fruit residue as livestock feed[J].Tropical Animal Health and Production, 2015, 47(3):557-561.  
[4] MELLO B L B, FERNANDES A M, DE OLIVEIRA T S, et al.Feed intake, digestibility, and energy contents in growing bull fed pineapple crop waste silage in different planes of nutrition[J].Tropical Animal Health and Production, 2021, 53(1):188.
[5] CUTRIM D O, ALVES K S, NEIVA J N M, et al.Replacement levels of elephant grass by moist pineapple by-product silage in diets of Santa Inês crossbred sheep:performance and digestibility[J].Tropical Animal Health and Production, 2013, 45(2):585-592.  
[6] RASHID M T, WEST J.Dairy wastewater treatment with effective microorganisms and duckweed for pollutants and pathogen control[C]//Wastewater Reuse-Risk Assessment, Decision-Making and Environmental Security.Dordrecht:Springer Netherlands, 2007:93-102.
[7] LASKOWSKA E, JAROSZ Ł, GRADZKI Z.The effect of feed supplementation with effective microorganisms (EM) on pro- and anti-inflammatory cytokine concentrations in pigs[J].Research in Veterinary Science, 2017, 115:244-249.
[8] 薛春胜, 刘瑞生, 徐建峰, 等.不同添加剂对陇东紫花苜蓿青贮品质的影响[J].草业科学, 2018, 35(2):456-462. XUE C S, LIU R S, XU J F, et al.Effect of different additives on the silage quality of Longdong alfalfa[J].Pratacultural Science, 2018, 35(2):456-462.(in Chinese)
[9] 谢晓华, 陈广仁, 杨保田.植物乳杆菌和EM菌剂对青贮大麦营养成分和发酵特性的影响[J].中国饲料, 2021(6):37-40. XIE X H, CHEN G R, YANG B T.Effects of Lactobacillus plantarum and EM microbial agents on nutrient composition and fermentation characteristics of silage barley[J].China Feed, 2021(6):37-40.(in Chinese)
[10] MENKE K H, RAAB L, SALEWSKI A, et al.The estimation of the digestibility and metabolizable energy content of ruminant feedingstuffs from the gas production when they are incubated with rumen liquor in vitro[J].Journal of Agricultural ence, 1979, 93(1):217-222.
[11] MAURICIO R M, MOULD F, OWEN E.Comparison of rumen liquor and faeces from cows as sources of micro-organisms for the in vitro gas production technique[J].Arquivo Brasileiro de Medicina Veterinária e Zootecnia, 1998, 50(5):569-572.  
[12] CUNNIFF P A, JEE M H.Official methods of analysis of AOAC international (16th ed)[J].Trends in Food Science and Technology, 1995, 6(11):382.
[13] 熊雪, 邵玲智, 董建新, 等.承德坝上御道口地区不同燕麦品种生产性能及饲用价值[J].草业科学, 2022, 39(7):1412-1418. XIONG X, SHAO L Z, DONG J X, et al.Comparison of production performance and feeding value of differentoat varieties in Yudaokou, Bashang, Chengde[J].Pratacultural Science, 2022, 39(7):1412-1418.(in Chinese)
[14] ROHWEDER D A, BARNES R F, Jorgensen N.Proposed hay grading standards based on laboratory analyses for evaluating quality[J].Journal of Animal Science, 1978, 47(3):747-759.  
[15] 庞凯悦, 杨英魁, 王迅, 等.高精料日粮对牦牛瘤胃体外发酵的研究[J].饲料研究, 2021, 44(14):5-8. PANG K Y, YANG Y K, WANG X, et al.Study on rumen fermentation of yak in vitro under high concentrate diet[J].Feed Research, 2021, 44(14):5-8.(in Chinese)
[16] 苏学军, 宗春燕, 王坤.饲料中亚硝酸盐的检测方法研究进展[J].饲料研究, 2020, 43(7):151-154. SU X J, ZONG C Y, WANG K.Research progress on detection methods of nitrite in feed[J].Feed Research, 2020, 43(7):151-154.(in Chinese)
[17] COCKBURN A, BRAMBILLA G, FERNÁNDEZ M L, et al.Nitrite in feed:from animal health to human health[J].Toxicology and Applied Pharmacology, 2013, 270(3):209-217.  
[18] 中华人民共和国国家质量监督检验检疫总局, 中国国家标准化管理委员会.饲料卫生标准:GB 13078-2017[S].北京:中国标准出版社, 2017. General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China.Hygienical standard for feeds:GB 13078-2017[S].Beijing:China Standards Press, 2017.(in Chinese)
[19] CUI K, QI M L, WANG S Q, et al.Dietary energy and protein levels influenced the growth performance, ruminal morphology and fermentation and microbial diversity of lambs[J].Scientific Reports, 2019, 9(1):16612.
[20] 葛剑, 杨翠军, 刘贵河, 等.混合比例和添加EM菌剂对紫花苜蓿和裸燕麦混贮品质的影响[J].浙江农业学报, 2015, 27(12):2093-2099. GE J, YANG C J, LIU G H, et al.Effects of mixed ratio and effective microorganism (EM) addition on the mixed silage quality of alfalfa (Medicago sativa L.) and naked oats (Avena nuda)[J].Acta Agriculturae Zhejiangensis, 2015, 27(12):2093-2099.(in Chinese)
[21] 马燕欣, 郎淑平.不同处理方法对晚稻秸秆青贮饲用价值的影响[J].浙江农业科学, 2017, 58(12):2262-2264, 2267. MA Y X, LANG S P.Effects of different treatments on the forage value of late rice straw silage[J].Journal of Zhejiang Agricultural Sciences, 2017, 58(12):2262-2267, 2267.(in Chinese)
[22] 韩建成, 陈水秀, 江杨, 等.不同微生物发酵剂对青贮甜玉米秸秆营养成分的影响[J].热带农业工程, 2016, 40(3):8-12. HAN J C, CHEN S X, JIANG Y, et al.Effects of different microbial fermentation agents on the nutritional components of sweet corn stalk silage[J].Tropical Agricultural Engineering, 2016, 40(3):8-12.(in Chinese)
[23] FALKNER L K, CASLER M D.Preference for smooth bromegrass clones is affected by divergent selection for nutritive value[J].Crop Science, 1998, 38(3):690-695.  
[24] 陈毅东, 张桂杰, 黄帅.西北荒漠区沙生植物瘤胃降解特性及饲用价值分析[J].饲料研究, 2021, 44(1):1-5. CHEN Y D, ZHANG G J, HUANG S.Analysis of rumen degradation characteristics and feeding value of psammophytes in desert area of Northwest China[J].Feed Research, 2021, 44(1):1-5.(in Chinese)
[25] 刘桂要.玉米秸杆青贮过程中微生物、营养成分及有机酸变化规律的研究[D].硕士学位论文.杨凌:西北农林科技大学, 2009:37. LIU G Y.Studies on the changes of microorganism, nutrits and organic acid during ensiling for corn straw[D].Master's Thesis.Yangling:Northwest A&F University, 2009:37.(in Chinese)
[26] 张增欣, 邵涛.丙酸对多花黑麦草青贮发酵动态变化的影响[J].草业学报, 2009, 18(2):102-107. ZHANG Z X, SHAO T.The effect of propionic acid addition on the dynamic fermentation changes of Italian ryegrass (Lolium multiflorum) silage[J].Acta Prataculturae Sinica, 2009, 18(2):102-107.(in Chinese)
[27] 芦岩, 张伶俐, 罗远琴, 等.不同比例棉秆和甜菜渣混合发酵产物的体外产气特性及发酵参数的研究[J].草业学报, 2020, 29(5):58-66. LU Y, ZHANG L L, LUO Y Q, et al.In vitro gas production characteristics and fermentation parameters of feedstuffs with varying proportions of cotton stalks and beet pulp[J].Acta Prataculturae Sinica, 2020, 29(5):58-66.(in Chinese)
[28] KHAZAAL K, DENTINHO M T, RIBEIRO J M, et al.Prediction of apparent digestibility and voluntary intake of hays fed to sheep:comparison between using fibre components, in vitro digestibility or characteristics of gas production or nylon bag degradation[J].Animal Science, 1995, 61(3):527-538.  
[29] 李杰, 李秋玫, 王立群, 等.秸秆生物处理技术研究(五)——尼龙袋法测定粗饲料养分瘤胃48 h的消失率[J].黑龙江畜牧兽医, 2000(4):17-18. LI J, LI Q M, WANG L Q, et al.Study of treating straw with biologic methods V——determining the disappear ratio within 48 h of nutrition in rumen using nylon bag method[J].Heilongjiang Journal of Animal Science and Veterinary Medicine, 2000(4):17-18.(in Chinese)
[30] 王志敬.放牧雷州山羊营养水平监测及不同精粗比日粮对雷州山羊舍饲效果研究[D].硕士学位论文.湛江:广东海洋大学, 2017. WANG Z J.Study on the nutritional level monitoring of grazing Leizhou goat and the effect of different concentrate to roughage abundance on feeding in Leizhou goat[D].Master's Thesis.Zhenjiang:Guangdong Ocean University, 2017.(in Chinese)
[31] 严学兵.牦牛对高寒牧区天然草地和人工草地牧草消化性的研究[D].硕士学位论文.兰州:甘肃农业大学, 2000. YAN X B.Digestibility of natural and cultivated grasslands by yaks in alpine pastoral region[D].Master's Thesis.Lanzhou:Gansu Agricultural University, 2000.(in Chinese)
[32] 赵亚波, 张腾龙, 张艳梅, 等.棉籽酶解蛋白对奶牛瘤胃液体外发酵参数及微生物区系的影响[J].动物营养学报, 2021, 33(6):3297-3308. ZHAO Y B, ZHANG T L, ZHANG Y M, et al.Effects of enzymatic hydrolysate of cottonseed protein on rumen fermentation and microflora of dairy cows in vitro[J].Chinese Journal of Animal Nutrition, 2021, 33(6):3297-3308.(in Chinese)
[33] ARAMBEL M J, BARTLEY E E, DUFVA G S, et al.Effect of diet on amino and nucleic acids of rumen bacteria and protozoa[J].Journal of Dairy Science, 1982, 65(11):2095-2101.  
[34] ILLIUS A W.Matching ruminant production systems with available resources in the tropics and sub-tropics:T. R. Preston & R. A. Leng. Renambul books, Armidale, NSW, 1987. ISBN 0-9588290-12[J].Agricultural Systems, 1989, 30(2):200-201.  
[35] 丁健, 张建刚, 王雅倩, 等.苜蓿皂苷对瘤胃原虫体外发酵特性的影响[J].中国畜牧杂志, 2013, 49(19):59-64. DING J, ZHANG J G, WANG Y Q, et al.Effects of alfalfa saponins on ruminal protozoal fermentation in vitro[J].Chinese Journal of Animal Science, 2013, 49(19):59-64.(in Chinese)
[36] 芦岩.体外产气与尼龙袋法筛选棉秆与甜菜渣混贮最佳组合的研究[D].硕士学位论文.石河子:石河子大学, 2020:17. LU Y.Study on screening the best combination of cotton stalk and sugar beet residue by in vitro gas production and nylon bag method[D].Master's Thesis.Shihezi:Shihezi University, 2020:17.(in Chinese)
Outlines

/