Effects of Glucose on Expression of Inflammation Related Genes in Dairy Cow Mammary Epithelial Cells Stimulated by Lipopolysaccharide

ZHANG Jinyou; SONG Jinting; LI Wei; ZHUANG Yulong; LI Changzhi; SONG Weihong

doi:10.3969/j.issn.1006-267x.2022.11.052

2022 , Vol. 34 >Issue 11: 7350 - 7360

DOI: https://doi.org/10.3969/j.issn.1006-267x.2022.11.052

RESEARCH PAPER

Effects of Glucose on Expression of Inflammation Related Genes in Dairy Cow Mammary Epithelial Cells Stimulated by Lipopolysaccharide

ZHANG Jinyou ,
SONG Jinting ,
LI Wei ,
ZHUANG Yulong ,
LI Changzhi ,
SONG Weihong

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1. College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing 163319, China;
2. Institute of Animal Husbandry and Veterinary of Heilongjiang Academy of Land Reclamation Science, Harbin 150038, China

Received date: 2022-05-24

Online published: 2022-11-14

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Abstract

The purpose of this experiment was to study the effects of glucose on the expression of inflammation related genes in dairy cow mammary epithelial cells stimulated by lipopolysaccharide (LPS), so as to provide basis for understanding the effects of different energy levels on the immune response of mammary epithelial cells. Mammary epithelial cells were isolated from the mammary glands of healthy dairy cows. After purification, the cells were cultured for 24 h under the conditions of 0.18, 0.50, 0.90 and 4.50 g/L glucose, respectively, and 1 μg/mL LPS was added to the culture medium containing the above four glucose concentrations for 24 hours. After culture, the cells were collected to extract RNA, and real-time fluorescence quantitative PCR was used to detect the mRNA relative expression levels of interleukin-8 (IL-8), induced nitric oxide synthase (iNOS), lactoferrin (LF), interleukin-6 (IL-6), transforming growth factor-β1 (TGF-β1) and tumor necrosis factor-α (TNF-α) of genes in cells. The results showed as follows:there were no significant differences in the mRNA relative expression levels of IL-8, iNOS and LF among the four glucose concentrations (P>0.05); the mRNA relative expression level of IL-6 g in 0.18 and 0.50 g/L glucose was significantly higher than that in 0.90 and 4.50 g/L glucose (P<0.05); the mRNA relative expression level of TGF-β1 in 0.18 and 0.50 g/L glucose was significantly higher than that in 4.50 g/L glucose (P<0.05); the mRNA relative expression level of TNF-α in 0.18 g/L glucose was significantly higher than that in 0.50 and 4.50 g/L glucose (P<0.05). The addition of 1 μg/mL LPS significantly extremely down-regulated the mRNA relative expression level of TGF-β1 in 0.90 and 4.50 g/L glucose (P<0.01), it stimulated the expression of the other five genes in varying degrees. With the decrease of glucose concentration, the stimulating effect of LPS on iNOS, LF and TNF-α gene expression was enhanced (P<0.05); with the decrease of glucose concentration, the stimulating effect of LPS on IL-8 and IL-6 gene expression was weakened (P<0.05). In conclusion, although the low concentration of glucose can enhance the ability of mammary tissue to eliminate pathogenic bacteria by promoting the expression of LF gene. However, low concentration of glucose can still cause more adverse effects on the immune function of mammary epithelial cells in dairy cows by reducing the expression of IL-8 and IL-6 genes to weaken the recruitment of neutrophils, and increasing the expression of TNF-α, TGF-β1 and iNOS to increase the chance of immunosuppression and cell damage in mammary epithelial cells. Therefore, the low concentration of glucose increases the possibility of mastitis in general.

Key words： dairy cows; mammary epithelial cells; lipopolysaccharide; glucose; inflammation

Cite this article

ZHANG Jinyou , SONG Jinting , LI Wei , ZHUANG Yulong , LI Changzhi , SONG Weihong . Effects of Glucose on Expression of Inflammation Related Genes in Dairy Cow Mammary Epithelial Cells Stimulated by Lipopolysaccharide[J]. Chinese Journal of Animal Nutrition, 2022 , 34(11) : 7350 -7360 . DOI: 10.3969/j.issn.1006-267x.2022.11.052

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