Effects of Different Concentrations of Short-Chain Fatty Acids on Immune Response, Tight Junction Protein and G Protein-Coupled Receptor 41 Expression in Bovine Rumen Epithelial Cells

ZHAN Kang; TAN Dejin; MENG Zitong; MA Xiaoyu; ZHAO Guoqi

doi:10.3969/j.issn.1006-267x.2022.11.053

2022 , Vol. 34 >Issue 11: 7361 - 7369

DOI: https://doi.org/10.3969/j.issn.1006-267x.2022.11.053

RESEARCH PAPER

Effects of Different Concentrations of Short-Chain Fatty Acids on Immune Response, Tight Junction Protein and G Protein-Coupled Receptor 41 Expression in Bovine Rumen Epithelial Cells

ZHAN Kang ,
TAN Dejin ,
MENG Zitong ,
MA Xiaoyu ,
ZHAO Guoqi

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College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China

Received date: 2022-05-05

Online published: 2022-11-14

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Abstract

To investigate the effects of different concentrations of short-chain fatty acids (SCFAs) on immune response, tight junction protein and G protein-coupled receptor 41 (GPR41) expression in bovine rumen epithelial cells (BRECs) and the relationships between GPR41 and pro-inflammatory factors, chemokines and tight junction proteins. Four experimental groups were designed with three replicates each. BRECs of the four experimental groups were cultured with 20, 50, 100 and 150 mmol/L SCFAs, and cellular total RNA were extracted after 24 h of culture. The results showed as follows:1) the mRNA expression level of pro-inflammatory factor tumor necrosis factor-α (TNF-α) at 100 and 150 mmol/L SCFAs was significantly higher than that at 20 and 50 mmol/L SCFAs (P<0.05). 2) The mRNA expression levels of CXC chemokine ligand 3 (CXCL3), CXC chemokine ligand 5 (CXCL5) and CXC chemokine ligand 8 (CXCL8) were gradually downregulated with the increase of SCFAs concentration, and they were lowest when BRECs incubated with 150 mmol/L SCFAs. The mRNA expression levels of GPR41 at the concentration of 100 mmol/L SCFAs was significantly higher than that at the concentrations of 20 and 50 mmol/L SCFAs (P<0.05). 3) When the concentration of SCFAs was 150 mmol/L, it had the lowest mRNA expression levels of tight junction proteins claudin-1 (Claudin-1) and zonula occludens-1 (ZO-1), which were significantly decreased compared with 20 and 50 mmol/L SCFAs (P<0.05). 4) Correlation analysis showed that GPR41 mRNA expression level showed a significant positive correlation with the pro-inflammatory factor TNF-α mRNA expression level (P<0.05). However, a significant negative correlation with the chemokines CXCL3, CXCL5 and CXCL8 mRNA expression levels (P<0.05), and a negative correlation trend with the tight junction proteins Claudin-1 and ZO-1 mRNA expression levels (0.05 ≤ P<0.10). In conclusion, high concentrations (100 and 150 mmol/L) of SCFAs can induce pro-inflammatory responses of BRECs, and inhibit chemokine expression and immune response, reduce the expression of tight junction proteins, and disrupt the barrier function of rumen epithelium.

Key words： bovine rumen epithelial cells; short-chain fatty acids; immune; chemokines; GPR41

Cite this article

ZHAN Kang , TAN Dejin , MENG Zitong , MA Xiaoyu , ZHAO Guoqi . Effects of Different Concentrations of Short-Chain Fatty Acids on Immune Response, Tight Junction Protein and G Protein-Coupled Receptor 41 Expression in Bovine Rumen Epithelial Cells[J]. Chinese Journal of Animal Nutrition, 2022 , 34(11) : 7361 -7369 . DOI: 10.3969/j.issn.1006-267x.2022.11.053

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