Astragalin Enhances Nuclear Factor E2 Related Factor 2 Gene Expression to Repair Liver Injury Induced by Exhaustive Exercise in Mice

ZHANG Wentian; WU Qiuxue; ZHU Kaiye; LUO Haijing; ZHANG Zhenzhu; SHAO Shuli; ZHANG Weiwei

doi:10.3969/j.issn.1006-267x.2022.11.057

2022 , Vol. 34 >Issue 11: 7402 - 7410

DOI: https://doi.org/10.3969/j.issn.1006-267x.2022.11.057

RESEARCH PAPER

Astragalin Enhances Nuclear Factor E2 Related Factor 2 Gene Expression to Repair Liver Injury Induced by Exhaustive Exercise in Mice

ZHANG Wentian ,
WU Qiuxue ,
ZHU Kaiye ,
LUO Haijing ,
ZHANG Zhenzhu ,
SHAO Shuli ,
ZHANG Weiwei

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1. School of Life Science and Agriculture and Forestry, Qiqihar University, Qiqihar 161006, China;
2. Key Laboratory of Resistant Genetic Engineering and Cold Biodiversity Conservation of Heilongjiang Province, Qiqihar 161006, China

Received date: 2022-04-20

Online published: 2022-11-14

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Abstract

To explore the repairing effect of astragalin on liver injury in exhaustive exercise mice, forty-eight 8-week-old specific-pathogen-free (SPF) grade C57BL/6J male mice were randomly divided into four groups:DMSO group, exhaustion exercise group, 50 mg/kg astragalin group and 100 mg/kg astragalin group. In addition to the DMSO group, the other three groups received acute exhaustive exercise for 4 h. After exhaustive exercise, the mice of the two astragalin groups were gavaged with 50 and 100 mg/kg BW astragalin, respectively, the mice of the exhaustion exercise group were gavaged with the same amount of normal saline, and the mice of the DMSO group were gavaged with 0.1% DMSO prepared in normal saline. The mice were killed one hour later, and the scavenging capacity of hydroxyl radical superoxide anion, glutathione content, the activities of glutathione reductase and glutathione peroxidase, and the proteins and genes expression of Nrf2, glutamate-cysteine ligase cysteine subunit (GCLC) and glutamate-cysteine ligase modifier subunit (GCLM) in liver of mice were determined. The results show that, compared with the DMSO group, after acute exhaustive exercise the scavenging capacity of ·OH and O₂^- in mice liver decreased by 54% (P<0.01) and 43% (P<0.01), respectively, the content of GSH decreased by 27% (P<0.05), the activities of GR and GSH-Px decreased by 32% (P<0.01) and 37% (P<0.05), respectively, and the mRNA and protein relative expression levels of Nrf2 and GCLM were significantly or extremely significantly decreased (P<0.05 or P<0.01). Compared with the exhaustive exercise group, the scavenging ability of ·OH in 50 and 100 mg/kg astragalin groups increased by 1.45 (P<0.01) and 1.25 times (P<0.01), respectively, the scavenging capacity of O₂^- increased by 1.78 (P<0.01) and 1.95 times (P<0.01), the content of GSH increased by 1.36 (P<0.05) and 2.21 times (P<0.01), the activity of GSH-Px increased by 1.54 (P<0.01) and 1.99 times (P<0.01), the activity of GR increased by 1.42 (P<0.01) and 1.62 times (P<0.01). Moreover, the mRNA and protein relative expression levels of Nrf2, GCLC and GCLM in liver of mice were significantly or extremely significantly increased compared with the exhaustive exercise group (P<0.05 or P<0.01). In conclusion, appropriate amount of astragalin can repair the injury of mouse liver caused by exhaustive exercise through up-regulating Nrf2, GCLC and GCLM genes expression.

Key words： astragalin; exhaustive exercise; liver injury

Cite this article

ZHANG Wentian , WU Qiuxue , ZHU Kaiye , LUO Haijing , ZHANG Zhenzhu , SHAO Shuli , ZHANG Weiwei . Astragalin Enhances Nuclear Factor E2 Related Factor 2 Gene Expression to Repair Liver Injury Induced by Exhaustive Exercise in Mice[J]. Chinese Journal of Animal Nutrition, 2022 , 34(11) : 7402 -7410 . DOI: 10.3969/j.issn.1006-267x.2022.11.057

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