Effects of Different Selenium Sources on Growth Performance and Intestinal Morphology, Antioxidant Function, Immune Function and Apoptosis of Yellow-Feathered Broilers

SHEN Yutian; ZHANG Xiaodong; ZHANG Ling; WANG Yongxia

doi:10.3969/j.issn.1006-267x.2022.12.023

2022 , Vol. 34 >Issue 12: 7711 - 7722

DOI: https://doi.org/10.3969/j.issn.1006-267x.2022.12.023

RESEARCH PAPER

Effects of Different Selenium Sources on Growth Performance and Intestinal Morphology, Antioxidant Function, Immune Function and Apoptosis of Yellow-Feathered Broilers

SHEN Yutian ,
ZHANG Xiaodong ,
ZHANG Ling ,
WANG Yongxia

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1. College of Animal Science and Technology·College of Veterinary Medicine, Zhejiang A&F University, Lin'an 311300, China;
2. College of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, China

Received date: 2022-05-06

Online published: 2022-12-15

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Abstract

This experiment was conducted to investigate the effects of different selenium sources on growth performance and intestinal morphology, antioxidant function, immune function and apoptosis of yellow-feathered broilers. A total of 540 one-day-old Lingnan yellow-feathered broilers were randomly divided into 3 groups with 6 replicates per group and 30 broilers per replicate. Broilers in Control group (CON) were fed a basal diet, and those in sodium selenite (SS) group and selenomethionine (SM) group were fed the basal diet supplemented with 0.15 mg/kg selenium (Se) of SS and SM, respectively. The experiment lasted for 56 days. The results showed as follows:1) compared with the CON group, diet supplemented with SS or SM significantly increased average daily gain (ADG) from 22 to 56 days and 1 to 56 days of age (P<0.05). The feed/gain (F/G) in any growth period in the SM group was significantly decreased compared with the CON group (P<0.05). From 22 to 56 days and 1 to 56 days of age, the ADG in the SM group was significantly higher than that in the SS group (P<0.05), while the F/G was significantly lower than that in the SS group (P<0.05). 2) Compared with the CON group, diet supplemented with SS or SM significantly increased (P<0.05) villus height (VH) and villus height/crypt depth ratio (V/C) in duodenum and jejunum (P<0.05), and significantly decreased crypt depth (CD) in duodenum and jejunum (P<0.05). In comparison with the SS group, the jejunum CD was significantly decreased (P<0.05), while the duodenum and jejunum V/C as well as jejunum VH were significantly increased in the SM group (P<0.05). 3) Compared with the CON group, diet supplemented with SS or SM significantly increased the activities of glutathione peroxidase (GPx), thioredoxin reductase (TrxR), heme oxygenase-1 (HO-1) and catalase (CAT) of duodenal mucosa as well as the activities of GPx, TrxR and NAD(P)H:dehydrogenase quinone 1 (NQO1) in jejunal mucosa (P<0.05). The GPx and TrxR activities of jejunal mucosa in SS the group were significantly higher than those in the SM group (P<0.05). The NQO1 and CAT activities of duodenal and jejunal mucosa as well as the total superoxide dismutase (T-SOD) and HO-1 activities of duodenal mucosa in SM group were significantly higher than those in the SS group (P<0.05). 4) The contents of malondialdehyde (MDA) and protein carbonyl (PC) of duodenal and jejunal mucosa as well as the content of 8-hydroxydeoxyguanosine (8-OHdG) of duodenal mucosa in the SS group were significantly lower than those in the CON group (P<0.05). The contents of MDA, PC and 8-OHdG of duodenal and jejunal mucosa in the SM group were significantly lower than those in the SS group and CON group (P<0.05). 5) The contents of immunoglobulin A (IgA) and immunoglobulin G (IgG) in serum as well as the content of secretory immunoglobulin A (sIgA) in jejunal mucosa in the SS group were significantly higher than those in the CON group (P<0.05). The contents of IgA, IgG and immunoglobulin M (IgM) in serum as well as the content of secretory immunoglobulin A (sIgA) in duodenal and jejunal mucosa in the SM group were significantly higher than those in the SS group and CON group (P<0.05). 6) Compared with the CON group, diet supplemented with SS or SM significantly elevated the B-cell lymphoma-2 (Bcl-2) content in jejunal mucosa (P<0.05), and significantly decreased the cysteine-aspartic acid protease-3 (Caspase-3) content in duodenal mucosa (P<0.05). SM group increased (P<0.05) the Bcl-2 content in duodenal and jejunal mucosa was significantly increased as well the Caspase-3 content in jejunal mucosa was significantly decreased in the SM group compared with the SS group (P<0.05). In conclusion, dietary supplementation of SS or SM can improve the growth performance and intestinal morphology, enhance the antioxidant function and immune function of yellow-feathered broilers, and then reduce the intestinal oxidative damage and apoptosis, and the effects of SM is better than those of SS.

Key words： broilers; selenomethionine; sodium selenite; intestinal tract; growth performance

Cite this article

SHEN Yutian , ZHANG Xiaodong , ZHANG Ling , WANG Yongxia . Effects of Different Selenium Sources on Growth Performance and Intestinal Morphology, Antioxidant Function, Immune Function and Apoptosis of Yellow-Feathered Broilers[J]. Chinese Journal of Animal Nutrition, 2022 , 34(12) : 7711 -7722 . DOI: 10.3969/j.issn.1006-267x.2022.12.023

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