Effects of Rosemary Extract on Growth Performance, Serum Biochemical Indices, Antioxidant Capacity and Intestinal Morphology of Broilers Challenged with Escherichia coli

HE Wenxiang; DENG Juying; HUANG Hui; MENG Tiantian; HE Jianhua; ZHOU Yingjun; ZOU Shengwen; XIAO Dingfu

doi:10.3969/j.issn.1006-267x.2022.12.025

2022 , Vol. 34 >Issue 12: 7735 - 7746

DOI: https://doi.org/10.3969/j.issn.1006-267x.2022.12.025

RESEARCH PAPER

Effects of Rosemary Extract on Growth Performance, Serum Biochemical Indices, Antioxidant Capacity and Intestinal Morphology of Broilers Challenged with Escherichia coli

HE Wenxiang ,
DENG Juying ,
HUANG Hui ,
MENG Tiantian ,
HE Jianhua ,
ZHOU Yingjun ,
ZOU Shengwen ,
XIAO Dingfu

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1. College of Animal Science and Technology, Hunan Agricultural University, Changsha 410128, China;
2. Geneham Pharmaceutical Co., Ltd., Changsha 410014, China;
3. Xiangya School of Pharmaceutical Sciences, Central South University, Changsha 410013, China;
4. Hunan Wenfeng Poultry Co., Ltd., Loudi 417700, China

Received date: 2022-05-11

Online published: 2022-12-15

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Abstract

This experiment was conducted to investigate the effects of dietary rosemary extract on growth performance, serum biochemical indices, antioxidant capacity and intestinal morphology of broilers challenged with Escherichia coli. One hundred and forty-four 7-day-old female yellow-feathered broilers were randomly divided into 3 groups with 6 replicates per group and 8 broilers per replicate. Broilers in control group (CON group) and Escherichia coli challenge group (EC group) were fed a basal diet, and those in rosemary extract+Escherichia coli challenge group (ECRE group) were fed the basal diet supplemented with 400 mg/kg rosemary extract. The experiment lasted for 28 days (8 to 35 days of age). At the 29 days of age, broilers in the CON group were fed 2 mL sterile normal saline, and those in the EC group and ECRE group were fed 2 mL Escherichia coli K88 solution (1.0×10⁹ CFU/mL) for 7 consecutive days. The results showed as follows:1) from 29 to 35 days of age, compared with the CON group, the average daily feed intake and average daily gain in the EC group were significantly decreased (P<0.05), and the diarrhea rate was significantly increased (P<0.05); compared with the EC group, the average daily gain in the ECRE group was significantly increased (P<0.05), and the feed/gain ratio and diarrhea rate were significantly decreased (P<0.05); in addition, the feed/gain ratio in the ECRE group was significantly lower than that in the CON group (P<0.05). 2) Compared with the CON group, the spleen index in the EC group was significantly decreased (P<0.05), while the liver index was significantly increased (P<0.05); compared with the EC group, the liver index in the ECRE group was significantly decreased (P<0.05). 3) Compared with the CON group, serum urea content and lactate dehydrogenase activity in the EC group were significantly increased (P<0.05), while serum glucose content was significantly decreased (P<0.05); compared with the EC group, serum glucose content and glutamic oxalacetic transaminase activity in the ECRE group were significantly increased (P<0.05). 4) Compared with the CON group, serum glutathione peroxidase (GSH-Px) and catalase (CAT) activities in the EC group were significantly decreased (P<0.05), while serum nitric oxide (NO) content was significantly increased (P<0.05); compared with the EC group, serum GSH-Px and CAT activities and total antioxidant capacity (T-AOC) in the ECRE group were significantly increased (P<0.05); moreover, serum T-AOC in the ECRE group was significantly higher than that in the CON group (P<0.05). 5) Compared with the CON group, ileum crypt depth in the EC group was significantly increased (P<0.05), while the ratio of villus height to crypt depth was significantly decreased (P<0.05); compared with the EC group, the ileum crypt depth in the ECRE group was significantly decreased (P<0.05), while the ratio of ileum villus height to crypt depth was significantly increased (P<0.05). In conclusion, the supplementation of 400 mg/kg rosemary extract can effectively alleviate the adverse effects of Escherichia coli challenge on broilers by improving growth performance, organ indexes, regulating serum biochemical indices, increasing antioxidant capacity and improving intestinal morphology of broilers.

Key words： rosemary extract; Escherichia coli; broilers; growth performance; antioxidant performance

Cite this article

HE Wenxiang , DENG Juying , HUANG Hui , MENG Tiantian , HE Jianhua , ZHOU Yingjun , ZOU Shengwen , XIAO Dingfu . Effects of Rosemary Extract on Growth Performance, Serum Biochemical Indices, Antioxidant Capacity and Intestinal Morphology of Broilers Challenged with Escherichia coli[J]. Chinese Journal of Animal Nutrition, 2022 , 34(12) : 7735 -7746 . DOI: 10.3969/j.issn.1006-267x.2022.12.025

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