RESEARCH PAPER

Effects of Threonine and Isoleucine on Growth Performance, Cecum Morphology, Cytokines and Microbiota of Wanxi White Geese

  • YANG Yanan ,
  • SUN Qi ,
  • XU Lianzhu ,
  • DING Yuancui ,
  • YUAN Yuchen ,
  • HU Qianqian ,
  • LI Shenghe ,
  • REN Man
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  • Anhui Key Laboratory of Animal Nutritional Regulation and Health, College of Animal Science, Anhui Science and Technology University, Fengyang 233100, China

Received date: 2022-05-31

  Online published: 2022-12-15

Abstract

This experiment was conducted to study the effects of threonine and isoleucine on growth performance, cecum morphology, cytokines and microbiota of Wanxi white geese. A total of 180 thirty-day-old Wanxi white geese were randomly divided into 3 groups with 3 replicates per group and 20 geese per replicate. The control group was fed a basal diet, the threonine group was fed the basal diet supplemented with 2.0 g/kg threonine, and the isoleucine group was fed the basal diet supplemented with 2.5 g/kg isoleucine. The experiment lasted for 60 days. The results showed as follows:1) the feed to gain ratio (F/G) during 61 to 90 days of age of threonine group and isoleucine group was significantly lower than that of the control group (P<0.01). 2) At 60 days of age, the villous heigh and crypt depth in cecum of isoleucine group was significantly higher than that of the control group (P<0.05 or P<0.01). At 90 days of age, the crypt depth in cecum of threonine group was significantly higher than that of the control group (P<0.05), and the villous heigh/crypt depth in cecum of threonine group and isoleucine group was significantly lower than that of the control group (P<0.05). 3) At 90 days of age, the cecum tumor necrosis factor-α (TNF-α) content of threonine group and isoleucine group was significantly lower than that of the control group (P<0.05), the contents of interferon-γ (IFN-γ) and interleukin-4 (IL-4) in cecum of threonine group was significantly higher than that of the control group (P<0.05). 4) At 90 days of age, the cecum Tenericutes relative abundance of isoleucine group was significantly higher than that of the control group (P<0.01), and the relative abundances of Oscillospira, Peptococcus and unspecified_S24_7 in cecum of threonine group were significantly higher than those of the control group (P<0.05). In conclusion, dietary 2.0 g/kg threonine or 2.5 g/kg isoleucine can improve the growth performance of Wanxi white geese to a certain extent, isoleucine has a great effect on the cecum morphology at 60 days of age, and threonine has a great effect on the cecum morphology at 90 days of age. Dietary 2.5 g/kg isoleucine increases the cecum Tenericutes relative abundance of Wanxi white geese, and dietary 2.0 g/kg threonine increases the relative abundances of Oscillospira, Peptococcus and unspecified_S24_7 in cecum of Wanxi white geese.

Cite this article

YANG Yanan , SUN Qi , XU Lianzhu , DING Yuancui , YUAN Yuchen , HU Qianqian , LI Shenghe , REN Man . Effects of Threonine and Isoleucine on Growth Performance, Cecum Morphology, Cytokines and Microbiota of Wanxi White Geese[J]. Chinese Journal of Animal Nutrition, 2022 , 34(12) : 7821 -7832 . DOI: 10.3969/j.issn.1006-267x.2022.12.033

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