Effects of Dietary Tea Polyphenols on Jejunum Morphology, Antioxidant Capacity and Expression of Tight Junction Protein-Related Genes in Magang Geese

WANG Yuchuan; LIN Tong; CHEN Ailing; MA Tingting; ZHONG Yajing; WANG Baodong; LIU Chunpeng; FU Jing

doi:10.3969/j.issn.1006-267x.2022.12.034

2022 , Vol. 34 >Issue 12: 7833 - 7843

DOI: https://doi.org/10.3969/j.issn.1006-267x.2022.12.034

RESEARCH PAPER

Effects of Dietary Tea Polyphenols on Jejunum Morphology, Antioxidant Capacity and Expression of Tight Junction Protein-Related Genes in Magang Geese

WANG Yuchuan ,
LIN Tong ,
CHEN Ailing ,
MA Tingting ,
ZHONG Yajing ,
WANG Baodong ,
LIU Chunpeng ,
FU Jing

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1. College of Animal Science and Technology, Zhongkai College of Agricultural Engineering, Guangzhou 510225, China;
2. Institute of Healthy Breeding and Innovation, Zhongkai College of Agricultural Engineering, Guangzhou 510225, China;
3. College of Animal Science and Technology, Northeast Agricultural University, Harbin 150030, China;
4. Agricultural Development Service Center of Liaoning Province, Shenyang 110032, China

Received date: 2022-05-07

Online published: 2022-12-15

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Abstract

The objective of this experiment was to investigate the effects of dietary tea polyphenols on the intestinal morphology, antioxidant capacity and expression of tight junction protein-related genes of 70-day-old geese. A total of 240 healthy 28-day-old geese (half male and half female) with the same body weight were randomly divided into 4 groups of 6 replicates, with 10 geese in each group. The doses of 0 (control), 100 (low dose), 500 (medium dose) and 1 000 mg/kg (high dose) tea polyphenols (98.7% purity) were respectively added to the diets of the geese. The acclimatization period lasted for 1 week, and the experimental period lasted for 5 weeks. The results showed as follows:1) compared with the control group, 500 mg/kg tea polyphenols in the diet significantly increased the villus height of jejunum in Magang geese (P<0.05); 100 and 500 mg/kg tea polyphenols in the diet significantly reduced the crypt depth (P<0.05) and significantly increased the villus height/crypt depth of jejunum in Magang geese (P<0.05). 2) Compared with the control group, 500 and 1 000 mg/kg tea polyphenols in the diet significantly increased the jejunal glutathione peroxidase (GSH-Px), total superoxide dismutase (T-SOD) and glutathione peroxidase 2 (GPX2) activities in Magang geese (P<0.05); 100 and 500 mg/kg tea polyphenols significantly reduced jejunal malondialdehyde (MDA) content in Magang geese (P<0.05); the jejunal lactate dehydrogenase (LDH) activity of Magang geese significantly decreased in each tea polyphenol treatment group (P<0.05). 3) Compared with the control group, 500 and 1 000 mg/kg tea polyphenols in the diet significantly increased the antioxidant-related genes mRNA relative expression levels of superoxide dismutase 1 (SOD1), glutathione peroxidase 1 (GPX1), GPX2 and heme oxygenase-1 (HO-1) in jejunal mucosa tissue (P<0.05). 4) Compared with the control group, 500 and 1 000 mg/kg tea polyphenols significantly increased the mRNA relative expression levels of tight junction protein genes zonula occludens-1 (ZO-1), Claudin-5 and Occludin in jejunal mucosa tissue (P<0.05); the mRNA relative expression levels of E-cadherin in Magang geese in each tea polyphenols treatment group significantly increased (P<0.05). In summary, it can be seen that the addition of suitable doses of tea polyphenols can promote the jejunum morphological development of Magang geese and improve the antioxidant capacity of jejunum, as well as the degree in intestinal wall tight junctions. The recommended additive dose of tea polyphenols is 500 mg/kg.

Key words： tea polyphenols; Magang goose; jejunum morphological structure; antioxidant capacity; tight junction protein gene expression

Cite this article

WANG Yuchuan , LIN Tong , CHEN Ailing , MA Tingting , ZHONG Yajing , WANG Baodong , LIU Chunpeng , FU Jing . Effects of Dietary Tea Polyphenols on Jejunum Morphology, Antioxidant Capacity and Expression of Tight Junction Protein-Related Genes in Magang Geese[J]. Chinese Journal of Animal Nutrition, 2022 , 34(12) : 7833 -7843 . DOI: 10.3969/j.issn.1006-267x.2022.12.034

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