Effects of Dietary Phytosterol on Growth Performance, Health Status and Muscle Texture of Juvenile Micropterus salmoides

WANG Yi; DONG Kaiyue; WU Jianjun; ZHAO Xumin; LI Luoxin; JIN Jiali; CHU Zhipeng; CHAI Yi; LIU Wei

doi:10.3969/j.issn.1006-267x.2022.12.048

2022 , Vol. 34 >Issue 12: 8007 - 8021

DOI: https://doi.org/10.3969/j.issn.1006-267x.2022.12.048

RESEARCH PAPER

Effects of Dietary Phytosterol on Growth Performance, Health Status and Muscle Texture of Juvenile Micropterus salmoides

WANG Yi ,
DONG Kaiyue ,
WU Jianjun ,
ZHAO Xumin ,
LI Luoxin ,
JIN Jiali ,
CHU Zhipeng ,
CHAI Yi ,
LIU Wei

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1. College of Agriculture/College of Animal Sciences, Yangtze University, Jingzhou 434020, China;
2. Key Laboratory of Freshwater Biodiversity Conservation, Ministry of Agriculture and Rural Affairs of China, Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan 430223, China;
3. College of Aquatic Science and Technology, Shanghai Ocean University, Shanghai 201306, China;
4. SUNHY Biological Co., Ltd., Wuhan 430074, China;
5. Zhejiang Huatai Biotechnology Co., Ltd., Jinhua 322005, China

Received date: 2022-05-12

Online published: 2022-12-15

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Abstract

This experiment was carried out to investigate the effects of dietary phytosterol on the growth performance, health status and muscle texture of largemouth bass (Micropterus salmoides). Phytosterol was supplemented into a basal diet with the levels of 0 (TZ0),22.5 (TZ22), 45.0 (TZ45), 67.5 (TZ67) and 90.0 mg/kg (TZ90), respectively. A total of 600 largemouth bass[initial average weight (5.01±0.06) g] were randomly divided into 5 groups, with 3 replicates in each group, and 40 experimental fish in each replicate. Five diets were fed according to the grouping design of the experiment. The feeding period was 8 weeks The results showed as follows:compared with TZ0 group,1) the diets supplemented with phytosterol had no significant effect on the growth performance and feed utilization of the experimental fish (P>0.05); 2) the crude lipid and muscle glycogen contents of whole body in TZ45, TZ67 and TZ90 groups were significantly decreased (P<0.05), the liver glycogen content of experimental groups was significantly decreased (P<0.05), while the water content of whole body and muscle in TZ22, TA67 and TZ90 groups was significantly increased (P<0.05); 3) the activities of aspartate aminotransferase and alanine aminotransferase in serum of experimental fish in TZ22 and TZ67 groups decreased significantly (P<0.05), serum glucose content in TZ45 and TZ90 groups increased significantly (P<0.05), and serum triglyceride content in TZ22, TZ67 and TZ90 groups increased significantly (P<0.05); 4) serum catalase (CAT) activity increased significantly in TZ45 and TZ67 groups (P<0.05), while serum malondialdehyde content and liver CAT activity decreased significantly in TZ90 group (P<0.05); 5) the hardness (except for TZ90 group), stringiness and adhesiveness of the muscle of the experimental fish were significantly improved (P<0.05); 6) the diets supplemented with phytosterol had no obvious effect on the liver structure of experimental fish, the width of hepatocytes in TZ90 group was significantly increased (P<0.05), and the redness (a^*) value of liver in TZ45 group was significantly increased (P<0.05); 7) there was no significant change in the characteristics of intestinal villi (P>0.05). Through intestinal slices, it could be found that the intestines of experimental fish in TZ0 group were obviously damaged, while the intestinal villi in TZ22, TZ45, TZ67 and TZ90 groups were complete, and the abnormal phenomenon of intestinal villi was not obvious. It is concluded that the diet supplemented with an appropriate amount of phytosterol can significantly improve its liver, intestinal health and muscle physical quality. Through the quadratic regression model to analyze its whole fish crude fat and liver glycogen, it can be concluded that the optimal addition level of phytosterol in the diet of largemouth bass is 59.2 to 64.0 mg/kg.

Key words： phytosterol; largemouth bass; growth; health; muscle texture

Cite this article

WANG Yi , DONG Kaiyue , WU Jianjun , ZHAO Xumin , LI Luoxin , JIN Jiali , CHU Zhipeng , CHAI Yi , LIU Wei . Effects of Dietary Phytosterol on Growth Performance, Health Status and Muscle Texture of Juvenile Micropterus salmoides[J]. Chinese Journal of Animal Nutrition, 2022 , 34(12) : 8007 -8021 . DOI: 10.3969/j.issn.1006-267x.2022.12.048

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